Photobiomodulation (PBM) promotes angiogenesis in-vitro and in chick embryo chorioallantoic membrane model

Abstract

The application of light in various therapeutic settings known as Photobiomodulation (PBM) is well established. Indications are the improvement of wound healing and tissue regeneration, scarring, and perfusion as well as pain therapy. Tissue perfusion is mandatory for successful wound healing. Nevertheless, there is a lack of mechanistic studies. We investigate the potential effect of PBM from light emitting diodes (LED) at 635 nm, 80 mW/cm2, 24 J/cm2 on angiogenesis in a two-part study: 1.) Investigation of the effect of PBM on the proliferation of endothelial cells and on vasculogenesis in a co-culture model of endothelial cells and stem cells. 2.) Investigation of the influence of PBM at chick egg chorioallantoic membrane (CAM) assays with fresh human skin xenografts. In both study phases, we observed a stimulating effect of PBM at 635 nm; in part 1: for proliferation of HUVEC (human umbilical vein endothelial cells) (25833 ± 12859 versus 63002 ± 35760 cells/well, p < 0.05, for cellular network formation (2.1 ± 2.1 versus 4.6 ± 3.5, p < 0.05) and for less cell compactness p = 0.01; in part 2: for the increase of number of vessel junctions per ROI (region of interest) (15.9 ± 2.6 versus 20.8 ± 5.4, p < 0.05). Our results suggest significant promotion of angiogenesis by PBM at 635 nm in vitro and in vivo.

Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring

“…Lasers with a wavelength of 808 nm have demonstrated deepest tissue penetration and, as a result, have been widely used for various brain disorders 1 , 2 , 26 , 47 , 74 , 75 . Numerous cells/tissues responded to PBM, including neurons 76 , microglia 77 , astrocytes 78 , embryonic rat brains 79 , mouse embryonic stem cells 80 , and chicken embryos 81 . Furthermore, 808 nm low-level laser light penetrated the rabbit skull and brain to a depth of 2.5-3 cm when placed upon the skin surface in previous reports 82 - 84 .…”

Digging deeper: structural background of PEGylated fibrin gels in cell migration and lumenogenesis

“…Moreover, inside a transparent gel, the cells' behavior and functioning can be reliably modulated via light irradiation. [24][25][26][27] It is well known that the mechanical properties of the microenvironment can inuence the cells' behavior, [28][29][30] and the matrix stiffness is claimed to be a crucial cue that regulates angiogenesis. 31 The structural difference between native and modied brin ensures changes in their elastic properties.…”

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

“…Thus, LLLT also potentiates the effect of VEGF on angiogenesis. Winter et al (34) used light emitting diode (LED) at 635 nm, 80 mW/cm 2 , 24 J/cm 2 to induce angiogenesis in HUVEC and chick embryo chorioallantoic membrane (CAM). The investigators found a significant increase in HUVEC proliferation and cellular network formation, with the CAM model showing a significant increase in the number of vessel junction.…”