Photobiomodulation and the expression of genes related to the JAK/STAT signalling pathway in wounded and diabetic wounded cells

Jere, Sandy Winfield; Houreld, Nicolette Nadene; Abrahamse, Heidi

doi:10.1016/j.jphotobiol.2020.111791

Cited by 14 publications

(13 citation statements)

References 34 publications

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“…Of the 46 genes that were upregulated, EGFR, JAK3, STAT1, STAT2, STAT3, STAT5A, and STAT6 were affected. Genes in both models that were downregulated included inhibitors of the JAK/STAT pathway [110]. Both these studies showed that PBM in the visible red spectrum (660 nm) with 5 J/cm 2 activates cellular signalling by regulating transcription, translation, and activation of proteins involved in the JAK/STAT signalling pathway in wounded and diabetic wounded fibroblast cells [21,110].…”

Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 98%

“…Epidermal growth factor (EGF) plays a critical role in wound healing and is involved in cell proliferation and migration [110]. The interaction between EGF and EGF receptor (EGFR) induces receptor dimerisation and tyrosine autophosphorylation, thereby initiating the JAK/STAT signalling pathway [111].…”

Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 99%

“…This led to activation of the JAK/STAT signalling pathway (increased phosphorylation of JAK2, STAT1, and STAT5), which ultimately led to increased cell proliferation, migration, and viability of diabetic wounded fibroblasts. In a similar study, Jere et al [110] irradiated wounded and diabetic wounded fibroblast cells (660 nm; 11 mW/cm 2 ; 5 J/cm 2 ) to profile for 84 JAK/STAT signalling-related genes. They reported that 19 genes were significantly regulated in wounded fibroblast cells (2 genes upregulated and 19 genes downregulated).…”

Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 99%

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Cellular Signalling and Photobiomodulation in Chronic Wound Repair

Leyane

Jere

Houreld

2021

IJMS

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Photobiomodulation (PBM) imparts therapeutically significant benefits in the healing of chronic wounds. Chronic wounds develop when the stages of wound healing fail to progress in a timely and orderly frame, and without an established functional and structural outcome. Therapeutic benefits associated with PBM include augmenting tissue regeneration and repair, mitigating inflammation, relieving pain, and reducing oxidative stress. PBM stimulates the mitochondria, resulting in an increase in adenosine triphosphate (ATP) production and the downstream release of growth factors. The binding of growth factors to cell surface receptors induces signalling pathways that transmit signals to the nucleus for the transcription of genes for increased cellular proliferation, viability, and migration in numerous cell types, including stem cells and fibroblasts. Over the past few years, significant advances have been made in understanding how PBM regulates numerous signalling pathways implicated in chronic wound repair. This review highlights the significant role of PBM in the activation of several cell signalling pathways involved in wound healing.

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Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 98%

Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 99%

Section: Immortalised Granulosa (Kgn) Cellsmentioning

confidence: 99%

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Cellular Signalling and Photobiomodulation in Chronic Wound Repair

Leyane

Jere

Houreld

2021

IJMS

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“…PBMT is a noninvasive effective tool without any adverse effects which modulates the molecular and cellular activities for therapeutic purposes [60] such as lymphoedema [61], stroke, Alzheimer's disease, lung inflammation, diabetic wound healing [60], tissue regeneration and chronic obstructive pulmonary disorder (COPD) [62]. Well-documented publications have shown that red and near-infrared (NIR) lights prompt tissue healing by downregulation of inflammatory cytokines and increased angiogenesis [63,64]. PBMT has been utilised in the management of viral infections by suppressing the virus replication and modulating the inflammatory cytokines [61].…”

Section: Photobiomodulation (Pbm) Therapy (Pbmt)mentioning

confidence: 99%

Phototherapy as a Rational Antioxidant Treatment Modality in COVID-19 Management; New Concept and Strategic Approach: Critical Review

et al. 2020

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The COVID-19 pandemic has taken the entire globe by storm. The pathogenesis of this virus has shown a cytokine storm release, which contributes to critical or severe multi-organ failure. Currently the ultimate treatment is palliative; however, many modalities have been introduced with effective or minimal outcomes. Meanwhile, enormous efforts are ongoing to produce safe vaccines and therapies. Phototherapy has a wide range of clinical applications against various maladies. This necessitates the exploration of the role of phototherapy, if any, for COVID-19. This critical review was conducted to understand COVID-19 disease and highlights the prevailing facts that link phototherapy utilisation as a potential treatment modality for SARS-CoV-2 viral infection. The results demonstrated phototherapy’s efficacy in regulating cytokines and inflammatory mediators, increasing angiogenesis and enhancing healing in chronic pulmonary inflammatory diseases. In conclusion, this review answered the following research question. Which molecular and cellular mechanisms of action of phototherapy have demonstrated great potential in enhancing the immune response and reducing host–viral interaction in COVID-19 patients? Therefore, phototherapy is a promising treatment modality, which needs to be validated further for COVID-19 by robust and rigorous randomised, double blind, placebo-controlled, clinical trials to evaluate its impartial outcomes and safety.

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“…Photobiomodulation (PBM) uses light in the form of LASERs (light amplification by stimulated emission of radiation) and LEDs (light-emitting diodes) to stimulate and affect physiological processes in organisms at the molecular and cellular level. PBM has been shown to affect numerous pathways and molecules important to the wound healing process, and recently, our lab has shown that PBM controls the transcription of genes and stimulates cellular signaling in diabetic wounds [ 1 , 2 ]. Wound healing comprises overlapping processes that occur in a certain sequence to ensure efficient tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Levels of Cyclooxygenase 2, Interleukin-6, and Tumour Necrosis Factor-α in Fibroblast Cell Culture Models after Photobiomodulation at 660 nm

Shaikh-Kader

Houreld

Rajendran

et al. 2021

Oxidative Medicine and Cellular Longevity

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Chemicals and signaling molecules released by injured cells at the beginning of wound healing prompt inflammation. In diabetes, prolonged inflammation is one of the probable causes for delayed wound healing. Increased levels of cyclooxygenase-2 (cox-2), interleukin–6 (IL-6), and tumour necrosis factor-alpha (TNF-α) are associated with the inflammatory response and in diabetes, and increased levels of these contribute to chronic wounds that do not heal. Rising levels of cox-2, IL-6, and TNF-α have also been associated with increased oxidative stress. Photobiomodulation (PBM) may impact wound healing processes by affecting the signaling pathways and molecules pertinent to tissue repair. In the present study, the effect of PBM (wavelength: 660 nm; energy density: 5 J/cm2) on levels of cox-2, IL-6, and TNF-α was determined in fibroblast cell culture models. Four WS1 models (normal, normal wounded, diabetic, and diabetic wounded) were irradiated at 660 nm, and the culture media was collected at 0, 24, and 48 h postirradiation. Cells that were not irradiated (0 J/cm2) served as the controls. The following parameters were determined postirradiation: cell morphology using light microscopy, cell viability using the Trypan Blue exclusion assay, and levels of the inflammatory markers cox-2, IL-6, and TNF-α were measured using ELISA. Cell migration increased in the wounded groups over the 48 h interval after PBM; viability improved postirradiation in the diabetic wounded groups at 0 and 24 h ( P ≤ 0.05 and P ≤ 0.01 , respectively); levels of cox-2 decreased in normal and diabetic wounded groups at 0 h ( P ≤ 0.001 ) and increased in the diabetic and diabetic wounded groups at 48 h postirradiation ( P ≤ 0.05 and P ≤ 0.01 , respectively), while levels of IL-6 decreased in the normal ( P ≤ 0.01 ), diabetic ( P ≤ 0.05 ), and diabetic wounded ( P ≤ 0.001 ) groups at 24 h and in the diabetic and diabetic wounded groups at 48 h ( P ≤ 0.05 ) postirradiation. TNF-α was decreased in the normal wounded groups ( P ≤ 0.05 ) at 48 h. Through its effect on decreased IL-6 levels in diabetic cell models, PBM at 660 nm may be successful at decreasing oxidative stress; however, the present study also found an increase in cox-2 levels at 48 h postirradiation.

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Photobiomodulation and the expression of genes related to the JAK/STAT signalling pathway in wounded and diabetic wounded cells

Cited by 14 publications

References 34 publications

Cellular Signalling and Photobiomodulation in Chronic Wound Repair

Cellular Signalling and Photobiomodulation in Chronic Wound Repair

Phototherapy as a Rational Antioxidant Treatment Modality in COVID-19 Management; New Concept and Strategic Approach: Critical Review

Levels of Cyclooxygenase 2, Interleukin-6, and Tumour Necrosis Factor-α in Fibroblast Cell Culture Models after Photobiomodulation at 660 nm

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