Photoacoustic imaging as a highly efficient and precise imaging strategy for the evaluation of brain diseases

Qiu, Ting; Lan, Yintao; Gao, Weijia; Zhou, Mengyu; Liu, Shiqi; Huang, Wenyan; Zeng, Su; Pathak, Janak L.; Yang, Bin; Zhang, Jian

doi:10.21037/qims-20-845

Cited by 32 publications

(25 citation statements)

References 116 publications

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“…Encouraged by the good NIR-responsive H 2 S release performance of the Pd-Su nanomedicine in aqueous solution, we then conducted further evaluation of the H 2 S release in 4T1 cancer cells under the trigger of 808-nm laser irradiation using the WSP-5 fluorescent probe [ 41 , 42 ]. Cell nuclei were stained by the 4,6-diamino-2-phenyl indole (DAPI), which shows fluorescence signal (blue) in nucleus under confocal laser light.…”

Section: Resultsmentioning

confidence: 99%

Sulourea-coordinated Pd nanocubes for NIR-responsive photothermal/H2S therapy of cancer

et al. 2021

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Background Photothermal therapy (PTT) frequently cause thermal resistance in tumor cells by inducing the heat shock response, limiting its therapeutic effect. Hydrogen sulfide (H2S) with appropriate concentration can reverse the Warburg effect in cancer cells. The combination of PTT with H2S gas therapy is expected to achieve synergistic tumor treatment. Methods Here, sulourea (Su) is developed as a thermosensitive/hydrolysable H2S donor to be loaded into Pd nanocubes through in-depth coordination for construction of the Pd-Su nanomedicine for the first time to achieve photo-controlled H2S release, realizing the effective combination of photothermal therapy and H2S gas therapy. Results The Pd-Su nanomedicine shows a high Su loading capacity (85 mg g−1), a high near-infrared (NIR) photothermal conversion efficiency (69.4%), and NIR-controlled H2S release by the photothermal-triggered hydrolysis of Su. The combination of photothermal heating and H2S produces a strong synergetic effect by H2S-induced inhibition of heat shock response, thereby effectively inhibiting tumor growth. Moreover, high intratumoral accumulation of the Pd-Su nanomedicine after intravenous injection also enables photothermal/photoacoustic dual-mode imaging-guided tumor treatment. Conclusions The proposed NIR-responsive heat/H2S release strategy provides a new approach for effective cancer therapy. Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Sulourea-coordinated Pd nanocubes for NIR-responsive photothermal/H2S therapy of cancer

et al. 2021

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“…In addition, the direction of the optical axis can be calculated using the recorded PA intensity and the polarization direction of the incident light (10). Similarly, the structure of the specimen under the test can be extracted using by PA imaging using two different linearly polarized lights.…”

Section: Principle Of Polarization Detectionmentioning

confidence: 99%

“…Photoacoustic computed tomography (PACT) utilizes laser excitation and acoustic detection to achieve high spatial resolution and considerable imaging depth in biological tissues (1,2). Handheld PACT has shown to be a promising technique for clinical applications, especially intraoperative diagnosis or treatment (3)(4)(5)(6)(7)(8)(9)(10). Usually, PACT assumes that the absorption coefficient of tissues is independent of polarization and thus uses unpolarized light for excitation.…”

Section: Introductionmentioning

confidence: 99%

Optical fiber-based handheld polarized photoacoustic computed tomography for detecting anisotropy of tissues

Ren¹,

Zhang²,

He³

et al. 2022

Quant Imaging Med Surg

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Background: Photoacoustic computed tomography (PACT) is a fast-developing biomedical imaging modality and has immense potential for clinical translation. It utilizes laser excitation and acoustic detection to achieve high spatial resolution and considerable imaging depth in biological tissues. Current PACT primarily treats the absorption coefficient of tissues as a scalar variable while reconstructing the image, which limits its use for anisotropic evaluation of the tissues. Thus, by incorporating polarized imaging methods to evaluate anisotropy, applications of PACT can be further enhanced. So far, dichroism-sensitive PACT has been suggested for polarization detection of biological tissues. However, this approach is unsuitable for intraoperative imaging, since high-power spatial light is needed for excitation, which is dangerous and inconvenient to operate. Thus, there is a need to develop a polarized PACT system suitable for clinical use.Methods: Herein, we have proposed a specially designed handheld polarized PACT (HP-PACT) system, which was designed to promote intraoperative anisotropy detection of biological tissues. Excitation light was delivered by an optical fiber and reshaped by a compact set of lenses at the output end of the optical fiber. A polarizer was applied to generate linearly polarized light, and the polarization direction was adjusted by simply rotating the half-wave plate. Photoacoustic imaging (PAI) using excitation with several different polarization directions was carried out. Optical axes and the structure of the anisotropic objects were obtained using the principle of polarization detection with the PAI.Results: We experimentally demonstrated the performance of HP-PACT by imaging both the polarized and unpolarized plastic films. The results showed that HP-PACT can successfully detect the direction of the optical axes of polarized plastic films and has the ability to image at different depths. When linearly polarized light with different polarization directions was used as excitation, PAI studies on a highly anisotropic bovine tendon and relatively low anisotropic mouse leg showed the structural differences between the 2 tissues. The quantified degrees of anisotropy of the bovine tendon and mouse legs were 0.6 and 0.3, respectively. Conclusions:The proposed HP-PACT is able to determine the anisotropic substances' optical axes and distinguish anisotropic substances from isotropic ones. Thus, HP-PACT has the potential for intraoperative diagnosis and treatment of anisotropic tissues, including nerves and tendons.

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“…The new detection approach of photoacoustic microscopy (PAM) (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) has been proposed and developed in dermatology to noninvasively visualize the epidermal melanin content and dermal microvasculature of human skin (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36). However, studies that have used PAM lack fine-scale visualization of multilayered skin structures (including of the stratum corneum, stratum basale, and dermis) and the distribution of endogenous pigments.…”

Section: Introductionmentioning

confidence: 99%

Quantitative multilayered assessment of skin lightening by photoacoustic microscopy

Wang¹,

Yang²,

Cheng³

et al. 2022

Quant Imaging Med Surg

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Background: With the emergence of various new skin-lightening products, there is an urgent need to scientifically evaluate the efficacy and toxicology of these products, and provide scientific guidance for their use based on physiological differences between individuals. Visualized imaging methods and quantitative evaluation criteria play key roles in evaluating the efficacy of skin-lightening products. In order to quantify the changes in the multilayered morphology and endogenous components of human skin before and after the use of lightening products, high-resolution three-dimensional (3D) imaging of human skin is required.Methods: In this study, photoacoustic microscopy (PAM; SSPM-532, Guangdong Photoacoustic Medical Technology Co., Ltd.) was used to capture the morphological structures of human skin and reveal skin components quantitatively. The efficacy and safety of skin-lightening products were evaluated by measuring skin melanin concentration and observing skin morphology. The melanin concentration in the epidermis was obtained by examining the linear relationship between photoacoustic (PA) signals. Further, the epidermal thickness and the melanin distribution were obtained in the cross-sectional (x-z) and lateral (x-y) images.Finally, the efficacy of skin-lightening products was evaluated according to the concentration and distribution of melanin in the epidermis, and the safety of cosmetics was assessed by observing the vascular morphology in the dermis.Results: PAM noninvasively could assess the multilayered morphological structures of human skin, which allowed for quantification of epidermal thickness and melanin concentration of different skin sites. Based on this, the efficacy and safety of skin-lightening products in multilayer structures were quantitatively evaluated.Conclusions: As a quantitative imaging method, PAM, has the potential to accurately evaluate the use of skin-lightening products. The method can also be extended to assessments within the larger field of aesthetic medicine.

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Photoacoustic imaging as a highly efficient and precise imaging strategy for the evaluation of brain diseases

Cited by 32 publications

References 116 publications

Sulourea-coordinated Pd nanocubes for NIR-responsive photothermal/H2S therapy of cancer

Sulourea-coordinated Pd nanocubes for NIR-responsive photothermal/H2S therapy of cancer

Optical fiber-based handheld polarized photoacoustic computed tomography for detecting anisotropy of tissues

Quantitative multilayered assessment of skin lightening by photoacoustic microscopy

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