Photo-toxic effects of 809-nm diode laser and indocyanine green on MDA-MB231 breast cancer cells

Bozkulak, Ozguncem; Yamaci, Rezan Fahrioglu; Tabakoglu, Ozgur; Gülsoy, Murat

doi:10.1016/j.pdpdt.2009.07.001

Cited by 28 publications

(20 citation statements)

References 18 publications

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“…22,26 Similarly, when ICG is exposed to near-infrared light, singlet oxygen is generated, 34 ICG is decomposed by singlet oxygen itself, and the decomposition products further decrease cell viability. 35 We also investigated the efficacy of the PDT with ICG injection and near-infrared light irradiation in rats with spinal metastasis.…”

Section: Discussionmentioning

confidence: 99%

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Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Funayama

Sakane

Abe

et al. 2012

Photomedicine and Laser Surgery

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Objective: The purpose of this study was to investigate the phototoxic effects of photodynamic therapy (PDT) with indocyanine green (ICG) and near-infrared light irradiation on rat mammary adenocarcinoma cells, and its therapeutic efficacy in a rat model of spinal metastasis. Background data: Although PDT has been successfully used as a non-radiation treatment for many malignancies, it has not yet been clinically applied for treating spinal metastasis. Methods: For the phototoxicity study, CRL-1666 cells were treated with PDT and cell viability was measured by WST-1 assay. For the efficacy study, 26 female Fischer 344 rats with spinal metastasis in the L6 vertebra were divided into three treatment groups: PDT with local injection of ICG (9 rats), PDT with systemic injection of ICG (10 rats), and no treatment or control (7 rats). Both the PDT groups received near-infrared light irradiation with a total energy of 10 J (1 W for 10 sec). The light was delivered directly through a single silica probe which was set on the left side of the L6 vertebral body. Hindlimb motor function was monitored according to the Basso-Beattie-Bresnahan (BBB) scale. Further, the observation periods were calculated to determine the survival time. Results: The PDT exerted immediate and persistent phototoxic effects. Furthermore, the PDT with local injection of ICG as well as systemic injection of ICG delayed the deterioration of paralysis and prolonged the observation period. Conclusions: PDT with ICG injection and near-infrared light irradiation could be an effective local adjuvant treatment for spinal metastasis.

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Section: Discussionmentioning

confidence: 99%

“…21 The phototoxic effect of ICG-enhanced therapy has been studied in various fields. [22][23][24][25][26][27][28] However, the strong binding of plasma proteins with ICG and its subsequent rapid clearance by the liver 21 results in no tumor selectivity after systemic administration.…”

mentioning

confidence: 99%

Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Funayama

Sakane

Abe

et al. 2012

Photomedicine and Laser Surgery

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“…Anticancer activity of ICG via the photodynamic effect has been studied in a number of cell lines, including HeCaT keratinocytes, HT-29 human colon cancer, SCL1 and SCL2 squamous cell carcinoma, N1 fibroblasts, human SKMEL 188 melanoma, mouse S91 melanoma, human skin Sk-Mel-28 melanoma, and MDA-MB231 breast cancer cells [13,14,15,18,20,21,36]. For example, with 24 h drug-incubation and a light dose of 24 J/cm 2 , the IC 50 values of ICG were reported to be 20.1, 43.9, 61.8, and 62.3 μM in HaCaT, SCL1, SCL2, and N1 cells, respectively [36].…”

Section: Resultsmentioning

confidence: 99%

“…In order to see whether this mechanism is responsible for the synergism observed in the present study, we performed an experiment in which the cells were treated with VP-16 immediately after laser irradiation (see Section 2.4). We would have observed synergistic enhancement of cytotoxicity, if the ICG-PDT treatment induced cellular damages, which were expected to last for hours following laser irradiation [21], had potentiated the cytotoxicity of VP-16. In contrast, the results in Figure 5 indicate that the strong synergy induced by the combination of ICG-PDT and VP-16 is not likely due to ICG-PDT-produced cellular damages but should involve direct or indirect interaction between ICG and VP-16 during the course of laser irradiation.…”

Section: Discussionmentioning

confidence: 99%

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Combination of Near Infrared Light-Activated Photodynamic Therapy Mediated by Indocyanine Green with Etoposide to Treat Non-Small-Cell Lung Cancer

Luo

Zhang

Lü

2017

Cancers

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Indocyanine green (ICG) has been reported as a potential near-infrared (NIR) photosensitizer for photodynamic therapy (PDT) of cancer. However the application of ICG-mediated PDT is both intrinsically and physiologically limited. Here we report a combination of ICG-PDT with a chemotherapy drug etoposide (VP-16), aiming to enhance the anticancer efficacy, to circumvent limitations of PDT using ICG, and to reduce side effects of VP-16. We found in controlled in vitro cell-based assays that this combination is effective in killing non-small-cell lung cancer cells (NSCLC, A549 cell line). We also found that the combination of ICG-PDT and VP-16 exhibits strong synergy in killing non-small-cell lung cancer cells partially through inducing more DNA double-strand breaks (DSBs), while it has a much weaker synergy in killing human normal cells (GM05757). Furthermore, by studying the treatment sequence dependence and the cytotoxicity of laser-irradiated mixtures of ICG and VP-16, we found that the observed synergy involves direct/indirect reactions between ICG and VP-16. We further propose that there exists an electron transfer reaction between ICG and VP-16 under irradiation. This study therefore shows the anticancer efficacy of ICG-PDT combined with VP-16. These findings suggest that ICG-mediated PDT may be applied in combination with the chemotherapy drug VP-16 to treat some cancers, especially the non-small-cell lung cancer.

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The Effects of Ultrasound and Light on Indocyanine‐Green‐Treated Tumour Cells and Tissues

Nomikou¹,

Sterrett

Arthur

et al. 2012

ChemMedChem

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Photodynamic therapy (PDT) is emerging as a treatment modality for the management of neoplastic disease. Despite considerable clinical success, its application for the treatment of deep-seated lesions is constrained by the inability of visible light to penetrate deeply into tissues. An emerging alternative approach exploits the fact that many photosensitisers respond to ultrasound, eliciting cytotoxic effects on target cells and tissues; this has become known as sonodynamic therapy (SDT). The objectives of this study were 1) to determine whether the IR-absorbing dye, indocyanine green (ICG), can be employed as a sonosensitiser and 2) to determine whether ultrasound can be used to enhance ICG-mediated PDT. Exposing ICG-treated mouse fibrosarcoma cells to ultrasound at an energy density of 30 J cm(-2) decreased cell viability by 65 %. Prior exposure of ICG-treated cells to light (λ 830 nm) and subsequent treatment with ultrasound led to a 90 % decrease in cell viability. In combination treatments a synergistic effect was observed at lower doses of ultrasound. Microscopic examination of cell populations treated with light or ultrasound demonstrated the production of intracellular reactive oxygen species (ROS). Using a mouse tumour model, treatment with light, ultrasound, or a combination thereof led to respective decreases in tumour growth of 42, 67, and 98 % at day 27 post-treatment. These results could provide a means of circumventing light-penetration issues that currently challenge the widespread use of PDT in the treatment of cancer.

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Photo-toxic effects of 809-nm diode laser and indocyanine green on MDA-MB231 breast cancer cells

Cited by 28 publications

References 18 publications

Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Combination of Near Infrared Light-Activated Photodynamic Therapy Mediated by Indocyanine Green with Etoposide to Treat Non-Small-Cell Lung Cancer

The Effects of Ultrasound and Light on Indocyanine‐Green‐Treated Tumour Cells and Tissues

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