Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Funayama, Toru; Sakane, Masataka; Abe, Tetsuya; Ochiai, Naoyuki

doi:10.1089/pho.2011.3080

Cited by 24 publications

(19 citation statements)

References 45 publications

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“…Our previous study proved the photodynamic effects of ICG in a rat model of bone metastasis from breast cancer [1]; however, no selective accumulation was observed in the tumor sites. To overcome the shortcomings of ICG-photodynamic therapy, Kimura et al developed nanoparticles that encapsulated ICG (ICG-lactosomes) [2] that could be integrated into inflammation or tumor sites in accordance with the enhanced permeability and retention (EPR) effect [3].…”

Section: Introductionmentioning

confidence: 77%

Indocyanine Green-lactosome and Near-infrared Light-based Intraoperative Imaging and Photodynamic Therapy for Metastatic Bone Tumors

Tsukanishi

Funayama²,

Ozeki

et al. 2014

J. Photopol. Sci. Technol.

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The reduction of local recurrence of metastatic spinal tuomors after intratumoral excision improves the quality of life. We have developed a novel photodynamic therapy (PDT) comprising an indocyanine green (ICG)-labeld nanocarrier (ICG-lactosome) and near-infrared light for use as an intraoperative adjuvant therapy. ICG-lactosomes accumulated in bone metastases from breast cancer because of enhanced permeability and retention effect. The cytotoxic effects of this novel PDT on human breast cancer cells were confirmed. In conclusion, ICG-lactosomes enable selective tumor imaging and represent a potential new PDT photosensitizer for adjuvant therapy during surgery for bone metastases from human breast cancer.

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Section: Introductionmentioning

confidence: 77%

Indocyanine Green-lactosome and Near-infrared Light-based Intraoperative Imaging and Photodynamic Therapy for Metastatic Bone Tumors

Tsukanishi

Funayama²,

Ozeki

et al. 2014

J. Photopol. Sci. Technol.

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“…Moreover, ICG injection and NIR light irradiation have photodynamic effects on spinal metastasis [28]. Thus, the combination of ICG-lactosome and NIR light irradiation could be a novel therapeutic system, including the intraoperative imaging modality and photodynamic therapy.…”

Section: Discussionmentioning

confidence: 99%

Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study

Funayama¹,

Sakane²,

Abe³

et al. 2012

TOBEJ

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Abstract:Marginal resection during resection of a spinal metastasis is frequently difficult because of the presence of important tissues such as the aorta, vena cava, and dura mater, including the spinal cord adjacent to the vertebral body. Thus, there is an urgent need for novel intraoperative imaging modalities with the ability to clearly identify bone metastasis. We have proposed a novel nanocarrier loaded with indocyanine green (ICG) (ICG-lactosome) with tumor selectivity attributable to its enhanced permeation and retention (EPR) effect. We studied its feasibility in intraoperative near-infrared (NIR) fluorescence diagnosis with ICG-lactosome for imaging spinal metastasis. A rat model of subcutaneous mammary tumor and a rat model of spinal metastasis of breast cancer were used. Fluorescence emitted by the subcutaneous tumors and the spinal metastasis were clearly detected for at least 24 h. Moreover, imaging of the dissected spine revealed clear fluorescence emitted by the metastatic lesion in the L6 vertebra while the normal bone lacked fluorescence. This study was the first report on NIR fluorescence imaging of spinal metastasis in vivo. NIR fluorescence imaging with ICG-lactosome could be an effective intraoperative imaging modality for detecting spinal metastasis.

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“…The L6 vertebra was exposed, and a hole was made with an 18-gage needle 1 mm left of the midline in this L6 vertebra. When the needle point reached a depth of 1.5 mm, the needle was rotated to create a 1 mm-wide hole (puncture model, n = 18) as Mantha reported 10,11) . A tumor section measuring 1 × 1 × 1 mm was excised from the donor rats and then implanted into the hole (metastasis model, n = 18).…”

Section: Introductionmentioning

confidence: 99%

Tumor Necrosis Factor-α Produced by Osteoclasts Might Induce Intractable Pain in a Rat Spinal Metastasis Model of Breast Cancer

Mazaki

Orita

Inage

et al. 2019

Spine Surg Relat Res

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Introduction Causes of pain due to spinal metastases have been insufficiently investigated. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were the focus of this study. Both are known as proinflammatory cytokines associated with the pathophysiology of pain syndromes 1 ) . It is well known that cancer cells produce these cytokines, but whether osteoclasts produce them as well remains unclear. We hypothesize that osteoclasts produce these cytokines; in other words, pain from spinal metastasis is stronger than pain from the primary tumor. Methods We made a rat spinal metastasis model of breast cancer (metastasis group) and models with a hole in the vertebrae (puncture group) and resected the vertebrae. Tartrate-resistant acid phosphatase (TRAP) staining was performed to reconfirm that osteoclasts increase in vertebrae with spinal metastasis. We then evaluated TNF-α and IL-6 expression using immunohistochemistry and real-time polymerase chain reaction (PCR). Results The results of TRAP staining showed that osteoclasts increase in metastatic vertebrae. The osteoclasts in the puncture models were TNF-α negative but were TNF-α positive in the metastasis model. The osteoclasts in the puncture models and metastasis model were both IL-6 positive. According to the real-time PCR results, TNF-α in vertebrae increased in the metastasis model, but IL-6 did not increase in the metastasis model compared with in the puncture model. Conclusions The number of osteoclasts is higher in the metastasis model. While TNF in the osteoclasts increased in the spinal metastasis model, IL-6 did not. This probably means that breast cancer affects TNF production in osteoclasts. This increase of TNF-α may lead to pain from spinal metastasis.

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Photodynamic Therapy with Indocyanine Green Injection and Near-Infrared Light Irradiation Has Phototoxic Effects and Delays Paralysis in Spinal Metastasis

Cited by 24 publications

References 45 publications

Indocyanine Green-lactosome and Near-infrared Light-based Intraoperative Imaging and Photodynamic Therapy for Metastatic Bone Tumors

Indocyanine Green-lactosome and Near-infrared Light-based Intraoperative Imaging and Photodynamic Therapy for Metastatic Bone Tumors

Intraoperative Near-infrared Fluorescence Imaging with Novel Indocyanine Green-Loaded Nanocarrier for Spinal Metastasis: A Preliminary Animal Study

Tumor Necrosis Factor-α Produced by Osteoclasts Might Induce Intractable Pain in a Rat Spinal Metastasis Model of Breast Cancer

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