Photo-thermally enhanced temperature gradient gel electrophoresis for DNA separation

Hou, Sichao

doi:10.17760/d20292405

Cited by 3 publications

(3 citation statements)

References 112 publications

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“…The specific heat c in J/kg • K for the 1.5%, 2.5%, 3.5% and 5% agar mixtures is, respectively, 6465 ± 452, 5827 ± 218, 4887 ± 477 and 4011 ± 386. The thermal conductivity of agarose hydrogel resembles, according to Hou [38], 0.65 (W/m • K) and decreases slightly to 0.51 (W/m • K) with the concentration increase from 0.5 wt% to 2 wt% of concentration.…”

Section: Skin-mimicking Phantomsmentioning

confidence: 81%

Comparative Analysis of Cooling Methods for Dynamic Infrared Thermography (DIRT)-Based Skin Cancer Diagnosis

Verstockt,

Thiessen,

Hoorens

et al. 2023

Applied Sciences

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Skin cancer is a significant global health issue, placing a growing burden on individuals and society. Conventional diagnostic methods like visual examination and biopsy have limitations in invasiveness and accuracy. As a result, alternative tools such as infrared thermography have gained attention in skin cancer diagnosis. Tissue-mimicking phantoms have been instrumental in facilitating research in this field, offering controlled environments. While they do not fully replicate human skin complexity, physical skin models provide stability, ease of fabrication, and control over properties. Agarose phantoms are employed in this study. This research focused on testing and comparing cooling techniques for human skin in the context of skin cancer diagnosis using dynamic infrared thermography. Six cooling methods were investigated: a cool pack, an aluminum medal, ice, alcohol, a vortex cooler and a Zimmer Cryo 6 cooler. The experimental setup involved an infrared camera (Optris Xi400) with microscope optics positioned above an agar phantom mimicking flat skin and an ulcerating skin lesion. Based on experiments conducted on the skin phantom, it was observed that convective cooling methods offered more consistent and uniform cooling. Conversely, conductive methods proved effective for flat objects but posed challenges in achieving uniform cooling for bulging skin or ulcerated lesions. Ice or alcohol were deemed unsuitable due to artifacts influencing the infrared radiation and thermal camera view. A decision matrix assessed cooling techniques based on criteria such as uniformity, repeatability, view obstruction, efficiency, workload, patient comfort, clinical suitability, noise exposure, consumables, additional equipment, and price. The Zimmer Cryo 6 cooler emerged as the most suitable cooling method after evaluating various factors.

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Section: Skin-mimicking Phantomsmentioning

confidence: 81%

Comparative Analysis of Cooling Methods for Dynamic Infrared Thermography (DIRT)-Based Skin Cancer Diagnosis

Verstockt,

Thiessen,

Hoorens

et al. 2023

Applied Sciences

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“…where q conv and q rad are convective and radiative heat fluxes applied to the surface boundaries, respectively; T air is the temperature of ambient air (T air ¼ 23 C); h is the heat transfer coefficient corresponding to free convection of air over a horizontal plate (h ¼ 7.2 W/(m 2 C)) [35]; r equals the Stefan-Boltzmann constant (r ¼ 5.7 Â 10 À8 W/m 2 C 4 ); and e equals the emissivity of Agar gel (e ¼ 0.85) [36].…”

Section: Thermal Boundary Conditionsmentioning

confidence: 99%

Computer simulations of an irrigated radiofrequency cardiac ablation catheter and experimental validation by infrared imaging

Rossmann

Motamarry

Panescu³

et al. 2021

International Journal of Hyperthermia

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Purpose: To develop and validate a three-dimensional (3-D) computer model based on accurate geometry of an irrigated cardiac radiofrequency (RF) ablation catheter with microwave radiometry capability, and to test catheter performance. Methods: A computer model was developed based on CAD geometry of a RF cardiac ablation catheter prototype to simulate electromagnetic heating, heat transfer, and computational fluid dynamics (blood flow, open irrigation, and natural convection). Parametric studies were performed; blood flow velocity (0-25 cm/s) and irrigation flow (0-40 ml/min) varied, both with perpendicular (PE) and parallel (PA) catheter orientations relative to tissue. Tissue Agar phantom studies were performed under similar conditions, and temperature maps were recorded via infrared camera. Computer model simulations were performed with constant voltage and with voltage adjusted to achieve maximum tissue temperatures of 95-105 C. Results: Model predicted thermal lesion width at 5 W power was 5.8-6.4 mm (PE)/6.5-6.6 mm (PA), and lesion depth was 4.0-4.3 mm (PE)/4.0-4.1 mm (PA). Compared to phantom studies, the mean errors of the computer model were as follows: 6.2 C(PE)/4.3 C (PA) for maximum gel temperature, 0.7 mm (10.9%) (PE)/0.1 mm (0.8%) (PA) for lesion width, and 0.3 mm (7.7%)(PE)/0.7 mm (19.1%) (PA) for lesion depth. For temperature-controlled ablation, model predicted thermal lesion width was 7-9.2 mm (PE)/8.6-9.2 mm (PA), and lesion depth was 4.3-5.5 mm (PE)/3.4-5.4 mm (PA). Conclusions: Computer models were able to reproduce device performance and to enable device evaluation under varying conditions. Temperature controlled ablation of irrigated catheters enables optimal tissue temperatures independent of patient-specific conditions such as blood flow.

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“…This method very simply employs one gradient gel turned 90 degrees to create a transverse pore gradient allowing the study of protein migration over a range of gel concentrations using only one gel. Transverse pore electrophoresis is more generally used for DNA analysis in various forms (Hou, 2018). Furthermore, although the transverse pore gradient gel method has previously been used in the PhastSystem, they proved this method works with a DNA Ladder as a standard and not protein (Buzas et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Expeditious Molecular Weight Determination of Native Proteins by Transverse Pore Gradient Polyacrylamide Gel Electrophoresis Using the PhastGel System

Singh¹,

Goykhman²,

Zhou³

et al. 2023

Vantage

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The Ferguson plot is well established method for the molecular weight determination of native proteins and protein complexes. The Ferguson plot requires running three or more gels of different polyacrylamide concentrations to determine the relationship between band migration and polyacrylamide gel concentration and using it solve for an unknown. Herein, we describe a new approach to a Ferguson plot that requires only one 8-25% gradient polyacrylamide gel, run as a transverse pore gradient, to provide the informational equivalent of 10-12 single concentration polyacrylamide gels. Using Cytochrome-C (14.4 kDa), E-GST (25 kDa), BSA (66 kDa) and BSA Dimer (132 kDa) as standards, we are able to determine the molecular weight of E-GST dimer with 9% experimental error in under 3 hours. This method has a significant advantage in reproducibility, efficiency, and cost effectiveness as compared to the standard method used for creating a Ferguson plot and is readily useable by any lab with a PhastSystem (GE LifeSciences).

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Photo-thermally enhanced temperature gradient gel electrophoresis for DNA separation

Cited by 3 publications

References 112 publications

Comparative Analysis of Cooling Methods for Dynamic Infrared Thermography (DIRT)-Based Skin Cancer Diagnosis

Comparative Analysis of Cooling Methods for Dynamic Infrared Thermography (DIRT)-Based Skin Cancer Diagnosis

Computer simulations of an irrigated radiofrequency cardiac ablation catheter and experimental validation by infrared imaging

Expeditious Molecular Weight Determination of Native Proteins by Transverse Pore Gradient Polyacrylamide Gel Electrophoresis Using the PhastGel System

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