Abstract:The Ferguson plot is well established method for the molecular weight determination of native proteins and protein complexes. The Ferguson plot requires running three or more gels of different polyacrylamide concentrations to determine the relationship between band migration and polyacrylamide gel concentration and using it solve for an unknown. Herein, we describe a new approach to a Ferguson plot that requires only one 8-25% gradient polyacrylamide gel, run as a transverse pore gradient, to provide the infor… Show more
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