Photo‐polymerized lauryl methacrylate monolithic columns for CEC using lauroyl peroxide as initiator

Abstract

Lauryl methacrylate (LMA)-ester based monolithic columns photo-polymerized using lauroyl peroxide (LPO) as initiator were prepared, and their morphological and CEC properties were studied. The composition of the polymerization mixture (i.e. ratios of monomers/porogenic solvents, 1,4-butanediol/1-propanol and LMA/crosslinker) was optimized. The morphological and chromatographic properties of LMA columns were evaluated by means of SEM pictures and van Deemter plots of PAHs, respectively. The polymerization mixture selected as optimal provided a fast separation of a mixture of PAHs with excellent efficiencies (minimum plate heights of 8.9-11.1 microm). Satisfactory column-to-column (RSD<4.5%) and batch-to-batch reproducibilities (RSD<6.3%) were achieved. The LMA columns photo-polymerized with LPO were compared with those prepared with AIBN. Using PAHs, alkylbenzenes and basic compounds for testing, the columns obtained with LPO gave the best compromise between efficiency, resolution and analysis time.

Preparation and characterization of octadecyl acrylate monoliths for capillary electrochromatography by photochemical, thermal, and chemical initiation†

“…This behavior was consistent with the SEM pictures of these monoliths (see Supporting Information Fig. S1) and with that previously described in several reports for thermal polymerization . An example of porous structure of these monoliths (prepared at 17:83 w/w 1,4‐butanediol/1‐propanol) is given in Fig.…”

“…A and B), where a decrease in the size of globules and voids was observed with decreasing 1,4‐butanediol content. A similar effect of the composition of porogens on the monolith morphology has been also reported for monoliths prepared by UV‐initiated polymerization .…”

Improved chromatographic performances of glycidyl methacrylate anion‐exchange monolith for fast nano‐ion exchange chromatography

“…Photopolymerized monoliths are considered as reproducible as thermally initiated polymers 43, 45 but because photopolymerization process is faster than thermal initiation (30 min compared with 24 h for this monolith), small deviations of the experimental setup during the initiation step could lead to high variations of the monolithic structure and poor reproducibility. Robustness of the monolith synthesis was evaluated through measurement of repeatability (capillary‐to‐capillary RSD) and reproducibility (batch‐to‐batch RSD) with three synthesis batches composed up to three monolithic capillaries.…”

Porous polymer monoliths: Amazingly wide variety of techniques enabling their preparation

“…It decomposes while irradiated at 365 nm to afford free radicals [132,137-142]. Since this initiator can also be used for the thermally initiated polymerization, comparative studies of both these approaches have been carried out [143,144]. They found only small differences in chromatographic performance of both types of monolithic capillary columns as demonstrated with separations of proteins shown in Fig.…”