Improved chromatographic performances of glycidyl methacrylate anion‐exchange monolith for fast nano‐ion exchange chromatography

Abstract

An efficient and reproducible photopolymerized poly(glycidyl methacrylate-co-ethylene dimethacrylate) was synthesized in Teflon-coated fused-silica capillaries (100 μm id) and functionalized by reaction of triethylamine with reactive epoxy groups. We report here the successful transfer of a standard polymerization mixture optimized for the thermally initiated synthesis of glycidyl-based monolith to photo-induced polymerization. The monolith obtained after optimization of the photo-initiation conditions was characterized in reverse-phase chromatography evaluating its suitability in terms of efficiency, retention and hydrodynamic permeability. Reproducibility of the photo-induced procedure was satisfactory with RSD below 6% for retention and efficiency and slightly higher for hydrodynamic permeability (12%). The functionalized generic support was then used in nano-ion-exchange chromatography. Efficiencies up to 75,000 plates/m, ion-exchange capacity of 8 nano-equivalents/cm of monolithic column, with a combination of a satisfactory hydrodynamic permeability allowed to perform fast separations of five inorganic anions in <3 min maintaining baseline resolution. The efficiency of the monolith was not retention-dependent, demonstrating its wide range of possible applications for highly retained anions.

Karakterisasi Kolom Monolit Methacrylate-Based Polymer Yang Dimodifikasi Dengan Trimetilamin

“…c. Modifikasi Penukar Anion dari Kolom Monolit Larutan 50% v/v trimetilamin dalam tetrahidrofuran dilewatkan kedalam monolit glisidil metakrilat dengan laju alir 2 μL/min selama 2 jam dan kemudian kolom diletakkan didalam ovendengan suhu 80ºC selama 5 jam. Setelah itu, kolom dibilas dengan metanol dengan laju alir 2 μL/menit selama 3 jam (Bruchet et al, 2011).…”

Quinine‐modified polymer monolithic column with reversed‐phase /strong anion‐exchange mixed‐mode for pressurized capillary electrochromatography

“…With the ring‐opening reaction of glycidyl methacrylate (GMA), various amine compounds such as phenylalanine, cystamine and epinephrine have been chemically decorated on GMA‐based monoliths and applied to chromatographic separation with mixed‐mode mechanisms including RP and weak anion–exchange (WAX) interactions . Based on the special aliphatic amine like triethylamine, cationic ammonium groups were formed via the nucleophilic reaction of tertiary amine with GMA, and the resultant positively charged monoliths were further used for strong anion–exchange (SAX) separations . Ring‐opening reactions of GMA with amine compounds have been studied and used extensively for the fabrication of MMCs.…”

A new zwitterionic electrochromatographic stationary phase based on poly(3-chloro-2-hydroxypropyl methacrylate-co-ethylene dimethacrylate) reactive monolith

“…Therefore, post‐modification has emerged as a versatile tool to easily tune the surface properties of monoliths . Reactive polymethacrylate monoliths were used as starting material and post‐functionalized with different chromatographic ligands to obtain stationary phases for different capillary electrochromatography (CEC) applications, affinity chromatography chiral separation, and various liquid chromatographic applications …”