Photo-induced protein modifications: a range of biological consequences and applications

Vera, Claudia Cecilia; Borsarelli, Claudio D.

doi:10.1007/s12551-023-01081-6

Cited by 3 publications

(4 citation statements)

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“…More focus has been placed on the creation of site-specific protein-cleaving agents, which is a remarkable challenge due to the exceptional stability of peptide bonds [ 2 ]. One of the advances in the use of artificial peptidases lies in the possibility of mapping and discerning binding sites on proteins and in the possibility of using them as molecular scissors to generate smaller fragments suitable for protein sequencing studies [ 3 ], the design of new DNA-targeted drugs [ 4 ] or even for the production of new biomaterials [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Mezzina,

Nicosia,

Barone

et al. 2024

Polymers

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The development of nanotools for chemical sensing and macromolecular modifications is a new challenge in the biomedical field, with emphasis on artificial peptidases designed to cleave peptide bonds at specific sites. In this landscape, metal porphyrins are attractive due to their ability to form stable complexes with amino acids and to generate reactive oxygen species when irradiated by light of appropriate wavelengths. The issues of hydrophobic behavior and aggregation in aqueous environments of porphyrins can be solved by using its PEGylated derivatives. This work proposes the design of an artificial photo-protease agent based on a PEGylated mercury porphyrin, able to form a stable complex with l-Tryptophan, an amino acid present also in the lysozyme structure (a well-known protein model). The sensing and photodegradation features of PEGylated mercury porphyrin were exploited to detect and degrade both l-Trp and lysozyme using ROS, generated under green (532 nm) and red (650 nm) light lasers. The obtained system (Star3600_Hg) and its behavior as a photo-protease agent were studied by means of several spectroscopies (UV-Vis, fluorescence and circular dichroism), and MALDI-TOF mass spectrometry, showing the cleavage of lysozyme and the appearance of several short-chain residues. The approach of this study paves the way for potential applications in theranostics and targeted bio-medical therapies.

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Section: Introductionmentioning

confidence: 99%

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Mezzina,

Nicosia,

Barone

et al. 2024

Polymers

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“…Singlet oxygen is often employed in biomaterial sciences to modify the functionalities or enhance the biocompatibility of biomolecules or therapeutic agents/medical devices. For example, it can be utilized in peptide and protien modification, − i.e., proteins–protein cross-linking and protein knockdown, conjugating fluorescence tags to biomolecules, and protein/enzyme immobilization on material surfaces for biocatalysis and biosensing, , etc. Singlet oxygen-responsive protein–protein cross-linking plays a vital role in various biochemical processes including environmental sensing, signal transduction, and maintaining cellular organization.…”

Section: Introductionmentioning

confidence: 99%

“…Singlet oxygen-responsive protein–protein cross-linking plays a vital role in various biochemical processes including environmental sensing, signal transduction, and maintaining cellular organization. For example, singlet oxygen-responsive protein–protein cross-linking is used for for wound suturing, tissue repair, tissue bioprinting where photoactivated materials derive the formation or degradation of chemical bonds with spatiotemporal control, and treatment of accommodative intraocular lenses to reverse presbyopia. ,, …”

Section: Introductionmentioning

confidence: 99%

“…For example, singlet oxygen-responsive protein–protein cross-linking is used for for wound suturing, tissue repair, tissue bioprinting where photoactivated materials derive the formation or degradation of chemical bonds with spatiotemporal control, 87 and treatment of accommodative intraocular lenses to reverse presbyopia. 84 , 86 , 88 …”

Section: Introductionmentioning

confidence: 99%

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Colloidal Quantum Dots as an Emerging Vast Platform and Versatile Sensitizer for Singlet Molecular Oxygen Generation

Khan,

Brito

et al. 2023

ACS Omega

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Singlet molecular oxygen ( 1 O 2 ) has been reported in wide arrays of applications ranging from optoelectronic to photooxygenation reactions and therapy in biomedical proposals. It is also considered a major determinant of photodynamic therapy (PDT) efficacy. Since the direct excitation from the triplet ground state ( 3 O 2 ) of oxygen to the singlet excited state 1 O 2 is spin forbidden; therefore, a rational design and development of heterogeneous sensitizers is remarkably important for the efficient production of 1 O 2 . For this purpose, quantum dots (QDs) have emerged as versatile candidates either by acting individually as sensitizers for 1 O 2 generation or by working in conjunction with other inorganic materials or organic sensitizers by providing them a vast platform. Thus, conjoining the photophysical properties of QDs with other materials, e.g., coupling/combining with other inorganic materials, doping with the transition metal ions or lanthanide ions, and conjugation with a molecular sensitizer provide the opportunity to achieve high-efficiency quantum yields of 1 O 2 which is not possible with either component separately. Hence, the current review has been focused on the recent advances made in the semiconductor QDs, perovskite QDs, and transition metal dichalcogenide QD-sensitized 1 O 2 generation in the context of ongoing and previously published research work (over the past eight years, from 2015 to 2023).

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Biophysical Reviews (ISSUE 4 2023): LAFeBS—highlighting biophysics in Latin America

Peluffo,

del V. Alonso,

Itri

et al. 2023

Biophys Rev

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Photo-induced protein modifications: a range of biological consequences and applications

Cited by 3 publications

References 71 publications

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Water-Soluble Star Polymer as a Potential Photoactivated Nanotool for Lysozyme Degradation

Colloidal Quantum Dots as an Emerging Vast Platform and Versatile Sensitizer for Singlet Molecular Oxygen Generation

Biophysical Reviews (ISSUE 4 2023): LAFeBS—highlighting biophysics in Latin America

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