Photo-hydrogen and lipid production from lactate, acetate, butyrate, and sugar manufacturing wastewater with an alternative nitrogen source byRhodobactersp.KKU-PS1

Abstract

Photo-hydrogen and lipid production from individual synthetic volatile fatty acids (VFAs) and sugar manufacturing wastewater (SMW) by Rhodobacter sp. KKU-PS1 with sodium glutamate or Aji-L (i.e., waste from the process of crystallizing monosodium glutamate) as a nitrogen source was investigated. Using individual synthetic VFAs, the maximum hydrogen production was achieved with Aji-L as a nitrogen source rather than sodium glutamate. The maximum hydrogen production was 1,727, 754 and 1,353 mL H2/L, respectively, using 25 mM of lactate, 40 mM of acetate and 15mM of butyrate as substrates. Under these conditions, lipid was produced in the range of 10.6–16.9% (w/w). Subsequently, photo-hydrogen and lipid production from SMW using Aji-L as nitrogen source was conducted. Maximal hydrogen production and hydrogen yields of 1,672 mL H2/L and 1.92 mol H2/mol substrate, respectively, were obtained. Additionally, lipid content and lipid production of 21.3% (w/w) and 475 mg lipid/L were achieved. The analysis of the lipid and fatty acid components revealed that triacyglycerol (TAG) and C18:1 methyl ester were the main lipid and fatty acid components, respectively, found in Rhodobacter sp. KKU-PS1 cells.

Microbial Lipid Production through Integrated Anaerobic-Aerobic Biotreatment Process: An Urban-Based Biorefinery Concept

“…They concluded that the substrate preference of the ASMC is in the order of glucose > acetic acid > mannitol > lactic acid. A similar order (acetic > butyric > lactic acid) was obtained by Assawamongkholsiri et al 50 when the SCCAs were individually used as carbon source for lipid production in Rhodobacter sp. KKU-PS1.…”

Microbial Upgrading of Acetate into Value-Added Products—Examining Microbial Diversity, Bioenergetic Constraints and Metabolic Engineering Approaches

“…As can be seen, the range of microorganisms for the production of lipids is diverse, including bacteria ( Rhodobacter sp. KKU-PS1), yeasts and fungi ( Y. lipolytica and Cryptococcus curvatus ), and even microalgae ( Auraniochytrium limacinum SR21) [ 97 , 98 , 99 , 100 ].…”