Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities
Abstract:Green synthesis of metal nanoparticles is reputed to have a robust range of biomedical applications. Silver nanoparticles (AgNPs) bio-fabricated using aqueous leaf extract of Annona muricata were characterized and evaluated for in-vitro antioxidant, lipid peroxidation inhibition, anti-diabetic and antimicrobial activities as well as cytotoxicity in human keratinocyte cells (HaCaT).The extract induced colour change of silver salt solution which absorbed at 420 nm and confirmed the formation of AgNPs. FTIR showe… Show more
“…When AgNPs’ concentration exceed 30 μg/mL or 10 μg/mL, it significantly inhibit HeLa and MCF-7 tumor cell growth respectively, showed good antiproliferative activity. There are some studies have proved that AgNPs have antiproliferative properties 44 – 46 . Due to their surface charges and sizes, the mechanism of antiproliferative activity mainly focuses on the following points: (1) inducing oxidative stress; (2) damaging DNA; (3) damaging mitochondria; (4) activating immune system; (5) inducing cell cycle arrest; (6) activating apoptosis.…”
Due to the increasing demand for eco-friendly, cost-effective and safe technologies, biosynthetic metal nanoparticles have attracted worldwide attention. In this study, silver nanoparticles (AgNPs) were extracellularly biosynthesized using the culture supernatants of Aspergillus sydowii. During synthesis, color change was preliminarily judge of the generation of AgNPs, and the UV absorption peak at 420 nm further confirms the production of AgNPs. Transmission electron microscopy and X-ray diffraction were also used to identify the AgNPs. The results shows that AgNPs has crystalline cubic feature and is a polydisperse spherical particle with size between 1 and 24 nm. Three main synthesis factors (temperature, pH and substrate concentration) were optimized, the best synthesis conditions were as follows 50 °C, 8.0 and 1.5 mM. In the biological application of AgNPs, it shows effective antifungal activity against many clinical pathogenic fungi and antiproliferative activity to HeLa cells and MCF-7 cells in vitro. Our research finds a new path to biosynthesis of AgNPs in an eco-friendly manner, and bring opportunity for biomedical applications in clinic.
“…When AgNPs’ concentration exceed 30 μg/mL or 10 μg/mL, it significantly inhibit HeLa and MCF-7 tumor cell growth respectively, showed good antiproliferative activity. There are some studies have proved that AgNPs have antiproliferative properties 44 – 46 . Due to their surface charges and sizes, the mechanism of antiproliferative activity mainly focuses on the following points: (1) inducing oxidative stress; (2) damaging DNA; (3) damaging mitochondria; (4) activating immune system; (5) inducing cell cycle arrest; (6) activating apoptosis.…”
Due to the increasing demand for eco-friendly, cost-effective and safe technologies, biosynthetic metal nanoparticles have attracted worldwide attention. In this study, silver nanoparticles (AgNPs) were extracellularly biosynthesized using the culture supernatants of Aspergillus sydowii. During synthesis, color change was preliminarily judge of the generation of AgNPs, and the UV absorption peak at 420 nm further confirms the production of AgNPs. Transmission electron microscopy and X-ray diffraction were also used to identify the AgNPs. The results shows that AgNPs has crystalline cubic feature and is a polydisperse spherical particle with size between 1 and 24 nm. Three main synthesis factors (temperature, pH and substrate concentration) were optimized, the best synthesis conditions were as follows 50 °C, 8.0 and 1.5 mM. In the biological application of AgNPs, it shows effective antifungal activity against many clinical pathogenic fungi and antiproliferative activity to HeLa cells and MCF-7 cells in vitro. Our research finds a new path to biosynthesis of AgNPs in an eco-friendly manner, and bring opportunity for biomedical applications in clinic.
“… 62 Antioxidants are vital molecules required to neutralize the generation of excessive free radicals in an aerobic organism and subsequently protect the body against prompted oxidative stress-related ailments. 55 , 63 The antioxidant potential of the synthesized AgNPs has been stated to have a relative advantage over the traditional antioxidant delivery system in terms of the targeted and controlled delivery, elevated bioavailability, and condensed protection of antioxidant agents. 55 , 64 Figure 8 Antioxidant ( A ) (DPPH assay) and ( B ) (Reducing power assay) effect of DtbP-AgNPs.…”
Section: Resultsmentioning
confidence: 99%
“… 55 , 63 The antioxidant potential of the synthesized AgNPs has been stated to have a relative advantage over the traditional antioxidant delivery system in terms of the targeted and controlled delivery, elevated bioavailability, and condensed protection of antioxidant agents. 55 , 64 Figure 8 Antioxidant ( A ) (DPPH assay) and ( B ) (Reducing power assay) effect of DtbP-AgNPs. Columns with different letters (a,b,c,d,e,f) are statistically significant at P < 0.05.…”
Section: Resultsmentioning
confidence: 99%
“…It is assumed that the AgNPs could have inhibited the specific enzymes (in this case αglucosidase) by binding to the other part of the enzyme, not to its active part and enzyme-substrate complex. 55 Earlier it was also reported that the AgNPs could have reduced the enzyme levels, which are in charge of catalyzing the hydrolysis of complex carbohydrates, and improved the rate of usage of glucose which might be the reason for the antidiabetic effect of the generated AgNPs. [56][57][58] In earlier research analogous results have been detailed for silver nanoparticles in terms of the α-glucosidase activity.…”
Section: Biological Activities Of the Biosynthesized Dtbp-agnpsmentioning
confidence: 99%
“…55,63 The antioxidant potential of the synthesized AgNPs has been stated to have a relative advantage over the traditional antioxidant delivery system in terms of the targeted and controlled delivery, elevated bioavailability, and condensed protection of antioxidant agents. 55,64…”
Section: Biological Activities Of the Biosynthesized Dtbp-agnpsmentioning
Background: The dragon tongue beans are a legume belonging to the Fabaceae family, are rich in protein, starch, fiber, and other micronutrients that have numerous health-promoting benefits. Its peel commonly the waste parts also contains lots of bioactive compounds. Materials and Methods: In the current research, dragon tongue bean peels (DtbP) extract is tested for the existence of phytochemicals. Ag nanoparticles are biosynthesized using DtbP extract. The generated DtbP silver nanoparticle characterization was accomplished using UV-Vis spectral analysis, FTIR spectral analysis, SEM analysis, EDX analysis, XRD analysis, zeta potential, and DLS study. Furthermore, comparative assessment on multi-biological activities of the biosynthesized Ag nanoparticles was accomplished by employing cytotoxicity (inhibition against HepG 2 cancer cells), antidiabetic (α-glucosidase inhibition assay), and antioxidant (free-radical scavenging) analysis. Results: The characterization result of the DtbP-AgNPs demonstrated that the AgNPs synthesized within 24 h. The AgNPs are nearly spherical. The biological effect assay of AgNPs displayed that DtbP-AgNPs is having significant cytotoxicity, antidiabetic, and moderate antioxidant effect. This study results as a whole report the biosynthesis of DtbP-AgNPs utilizing the legume dragon tongue bean waste peel and assessment of their multiple biological activities. The synthesized DtbP-AgNPs could serve as a potential candidate in the pharmaceutical industries in the formulation of drugs for the treatment of several medical ailments concerning cancer, diabetes, etc.
In the present study, aqueous leaf extract of Strobilanthes cordifolia J.R.I.Wood was combined with silver nitrate to synthesis silver nanoparticles (AgNPs). The AgNPs were characterized using visible spectroscopy (UV), X-ray diffraction (XRD), Fourier transform infrared spectrophotometer (FTIR), scanning electron microscope (SEM), energy dispersive X-ray (EDaX), particle size analysis, and transmission electron microscope (TEM). The UV spectrum absorption peak occurred at 438 nm. The FTIR analysis of the AgNPs indicated the presence of functional groups such as aldehyde, alkenes, and carboxylic acids. The crystalline structure of AgNPs was confirmed by XRD. The AgNPs have a spherical shape according to SEM. The AgNPs components composition was confirmed by EDaX. The particle size distribution of AgNPs is monodispersion in the range at 42.54 nm. TEM demonstrated the AgNPs size to be between 11.35 and 34.90 nm. The AgNPs exhibited good antibacterial property against Escherichia coli and Staphylococcus aureus. The antioxidant activity of the AgNPs was represented by increased DPPH, ABTS, and H 2 O 2 activities.The antidiabetic activity of the AgNPs was indicated by the inhibition of α-amylase and α-glycosidase and antiinflammatory highest albumin denaturation and HRBC membrane stabilization properties. Further, the AgNPs also significantly inhibited the MCF-7 cell lines. These results clearly suggest that the synthesized AgNPs using S. cordifolia leaves could have several potential biomedical applications.
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