Characterization and Evaluation of Multiple Biological Activities of Silver Nanoparticles Fabricated from Dragon Tongue Bean Outer Peel Extract

Patra, Jayanta Kumar; Shin, Han‐Seung; Das, Gitishree

doi:10.2147/ijn.s290037

Cited by 7 publications

(2 citation statements)

References 54 publications

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“…The IC 50 values of B, A, E, and OPD are as follows 38.56, 42.19, 44.84, and 47.23 μg/mL, respectively. On the other hand, the Ag nanoparticles synthesized from dragon tongue bean peels (DtbP-AgNPs) possessed an IC 50 value of 88.37 μg/mL as compared to gold nano-bioconjugate B with 38.56 μg/mL, which indicated that B can act as an efficient antioxidant agent. Ascorbic acid served as a standard and exhibited 83.61% inhibition, which is comparable with gold nano-bioconjugate B with 81.25% inhibition.…”

Section: Results and Discussionmentioning

confidence: 99%

Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies

2021

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Plant secondary metabolites such as flavonoids demonstrate high degrees of antioxidant, anti-inflammatory, and anticancer activities. Among flavonoids, quercetin plays an important role in inflammation by downregulating the level of various cytokines. Thereby, in this work, onion (Allium cepa) peel was successfully utilized for the synthesis of gold nano-bioconjugates acting as a natural therapeutic drug. In this process, crude onion peel extract was first divided into different fractionates, namely, ethyl acetate, butanol, methanol, and water, and they were subjected to various preliminary studies of antioxidant activities. The ethyl acetate fractionate shows high antioxidant activities in all the assays. The bioactive components were identified and found to contain a high amount of quercetin as confirmed by liquid chromatography with tandem mass spectrometry and high-performance liquid chromatogrpahy. Three gold nano-bioconjugates were prepared with different concentrations of the ethyl acetate fractionate. Various biochemical anti-inflammatory assays were carried out and compared with the active ethyl acetate fraction of the onion peel drug (OPD). The cytotoxicity of the nano-bioconjugate system and the OPD was checked in the myoblast L6 cell line from skeletal muscle tissues to evaluate the toxicity. All the three nano-bioconjugates A, B, and E demonstrated high percentages of cell viability, viz., 73.07, 72.3, and 69.15%, respectively, at their highest concentration of 200 μg/mL. The OPD also showed 88.56% cell viability with no toxic effects in the myoblast L6 cell line from skeletal muscle tissues. The reactive oxygen species reduction of nano-bioconjugate B showed a marked reduction of 76.77% at a maximum concentration of 200 μg/mL, whereas the OPD showed 68.17%. Hence, through this work, a cheap source of nano-bioconjugates is developed, which can act as a potent antioxidant and anti-inflammatory agent and are more active in comparison to the OPD alone.

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Section: Results and Discussionmentioning

confidence: 99%

Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies

2021

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“…These analytical methods such as UV-VIS spectral study, energy-dispersive X-ray spectroscopy analysis, Fourier transform infrared spectroscopy analysis, scanning electron microscopy study, X-ray powder diffraction study, Dynamic light scattering, and zeta potential were used to characterize the BdkR-AgNPs according to standard techniques. 5 , 34 , 35 The preliminary synthesis of the BdkR-AgNPs was confirmed by scanning between 300 and 700 nm using the UV-VIS spectrophotometer (Multiskan Go; Thermo Scientific, Waltham, MA, USA). The morphology of the synthesized BdkR-AgNPs was characterized by the SEM machine (Hitachi S-3000N, Tokyo, Japan) and the elemental composition was determined by an EDX machine attached to the SEM.…”

Section: Methodsmentioning

confidence: 99%

Key Health Benefits of Korean Ueong Dry Root Extract Combined Silver Nanoparticles

Das¹,

Shin²,

Patra³

2022

IJN

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Introduction Nowadays, in nanotechnology and material science, biosynthesis of the metal nanoparticle is a promising approach. Methods In the current research, the extract of the Korean Ueong dry root (BdkR), which belongs to the Asteraceae family, was used as a reducing and capping agent, for the green synthesis of the BdkR-Ag nanoparticles in a cost-effective and highly efficient manner. In this study for the reaction measures, UV-Vis spectroscopy was applied. SEM, EDX, FTIR, XRD, mean size distribution, and zeta potential were used for the characterization of the green synthesized BdkR-AgNPs. In the beginning, the primary phytochemical screening of BdkR extract was estimated and the cytotoxicity, antidiabetic, antioxidant, and antibacterial activities of the green synthesized BdkR-AgNPs were evaluated. Results According to the results, the BdkR extract is rich in various phytochemicals and the generated AgNPs were crystalline in nature. The surface plasmon resonance value of the BdkR-AgNPs was 444 nm confirming the synthesis of AgNPs. The BdkR-AgNPs displayed four clear diffraction peaks at 2 theta angles (38.22); (46.15); (64.88); (76.83), respectively, which are equivalent to (111), (200), (220) and (311). The obtained nanoparticles have a zeta potential of −17.0 mV. Furthermore, the generated BdkR-AgNPs exhibited considerable antidiabetic effect in terms of the inhibition of α-glucosidase with a maximum inhibition value of 95.41% at 5.0 µg/mL and more than 86% inhibition at 2.5 µg/mL and the estimated IC 50 value was found to be 0.653 µg/mL. Further, it also displayed a significant cytotoxicity activity against the HepG 2 cancer cell lines at 10 µg/mL and 100 µg/mL concentrations with 86% and 88% of inhibition, respectively. Besides this, the synthesized AgNPs also displayed promising antioxidant activities in terms of the DPPH (IC 50 value - 56.26 µg/mL), ABTS (IC 50 value - 171.43 µg/mL) and reducing power (IC 0.5 value - 227.42 µg/mL). Discussion The multipotential effects of the synthesized BdkR-AgNPs might be attributed to the presence of the bioactive compounds in the BdkR extract that acted as the capping and reducing agent in the synthesis process. The green synthesized BdkR-AgNPs exhibited promising bioactive potential for their future applications in the food and biomedical field.

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Fluorescent Carbon Dots from Vegetable and Fruit Wastes and Their Applications

Çeşme

Eskalen

Başkaya

2022

Fruits and Vegetable Wastes

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Characterization and Evaluation of Multiple Biological Activities of Silver Nanoparticles Fabricated from Dragon Tongue Bean Outer Peel Extract

Cited by 7 publications

References 54 publications

Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies

Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies

Key Health Benefits of Korean Ueong Dry Root Extract Combined Silver Nanoparticles

Fluorescent Carbon Dots from Vegetable and Fruit Wastes and Their Applications

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