Photo- and Metabolite Regulation of the Synthesis of Ribulose Bisphosphate Carboxylase/Oxygenase and the Phycobiliproteins in the Alga <i>Cyanidium caldarium</i>

Steinmüller, Klaus; Zetsche, Klaus

doi:10.1104/pp.76.4.935

Cited by 32 publications

(14 citation statements)

References 15 publications

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“…In the eucaryotic algae Cyanidium caldarium (Steinmuller & Zetsche 1984) and Chlorogonium elongatum (Steinbib & Zetsche 1986) the synthesis of the RuBPCase protein and mRNA was promoted by illumination. Yet transcription of the rbcL-rbcS operon in a freshwater heterocystous cyanobacterium, Anabaena sp., appears to be constitutively expressed (Nierzwicki-Bauer et al 1984).…”

Section: Discussionmentioning

confidence: 99%

Ribulose bisphosphate carboxylase gene expression in subtropical marine phytoplankton populations

Pichard¹,

Frischer²,

Paul³

1993

Mar. Ecol. Prog. Ser.

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Oceanic phytoplankton are known to fix CO2 primarily through the action of the enzyme ribulose-1,5-bisphosphate carboxylase (RuBPCase). The amino acid and nucleotide sequence of the large subunit of this enzyme have been conserved across the evolution of the chlorophytic plants (from cyanobacteria to higher plants via green algae) with approximately 80 and 70 % homology at the amino acid and nucleotide levels respectively. To understand the molecular regulation of this enzyme in phytoplankton, we have measured levels of the RuBPCase large subunit (rbcL) mRNA and DNA, in combination with rates of photosynthetic CO2 fixation, autofluorescent cell counts, and chlorophyll a in natural phytoplankton communities of Tampa Bay (Flonda, USA) and the southeastern Gulf of Mexico. We measured rbcL mRNA, rbcL DNA, and the ratio of rbcL mRNA to rbcL DNA by extracting RNA and DNA and probing the extracts with the Synechococcus PCC 6301 rbcL gene as a probe. Additionally, rbcL mRNA was amplified from certain samples using a reverse transcriptase-linked polymerase chain reaction procedure. In a transect from Tampa Bay seaward, levels of rbcL mRNA decreased 3-to 8-fold from the estuarine environment to the offshore environment, and followed similar trends as photosynthetic CO2 fixation, picocyanobacterial counts, and chlorophyll a. In an offshore vertical profile, the subsurface maximum in the rbcL mRNA/DNA ratio coincided with the 60 m maximum in photosynthetic assimilation rates. In a die1 study using 150 1 of offshore water in a deck-top incubator, the rbcL mRNA.DNA ratio was nearly an order of magnitude greater (76 ng mRNA ng-' DNA) during the light period than in the dark (17 ng mRNA ng-' DNA). Likewise, the photosynthetic rate (PB) at constant illun~ination was highest during daylight hours (6.2 pg C pg-' chl a h-') and lowest at night (1.9 pg C pg-' chl a h-'). These results indicate that (1) carbon fixation in oceanic phytoplankton may be transcriptionally regulated at the level of the RuBPCase gene and (2) that the quantitatlon of particular target mRNAs and DNAs is an effective means to study the regulation of conserved gene functions in natural water column microbial populations.

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Section: Discussionmentioning

confidence: 99%

Ribulose bisphosphate carboxylase gene expression in subtropical marine phytoplankton populations

Pichard¹,

Frischer²,

Paul³

1993

Mar. Ecol. Prog. Ser.

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“…In several algae, the synthesis o f ribulose 1,5 bisphosphate carboxylase/oxygenase (rubisco) in the light is inhibited in the presence o f metabolizable carbon c o m p o u n d s [31,32,43,44], and in Chlorogonium, this control has been shown to be effected at the level o f m R N A for the nuclear-encoded small subunit [32,43].…”

Section: Acetate Inhibits the Light-induced Increase In Cabli-1 M R Nmentioning

confidence: 99%

Expression of a gene for a light-harvesting chlorophyll a/b-binding protein in Chlamydomonas reinhardtii: effect of light and acetate

Kindle

1987

Plant Mol Biol

109

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In Chlamydomonas reinhardtii, the chlorophyll a/b-binding proteins of photosystem II are encoded in the nucleus by a small family of genes. We have studied the expression of one gene, which we call cabII-1, in a green-in-the-dark strain, which can synthesize chlorophyll in the dark or light, and in a yellow-in-the-dark mutant strain, which is able to make chlorophyll only in the light. In light/dark synchronized cultures of both strains, cabII-1 mRNA abundance increases during the first 6 h of a 12-h light phase, remains high for several hours, then declines. A variety of illumination conditions have been used to analyze the cabII-1 mRNA increase: continuous or intermittent red, blue, or white light, with or without 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU), an inhibitor of photosystem II. Our results suggest that light induces increased cabII-1 transcript abundance in two ways: 1) by virtue of its role in the light reactions of photosynthesis and 2) by a blue lightstimulated mechanism which is independent of photosynthesis.We have also examined the role of acetate in regulating cabII-1 mRNA levels in the dark. In both green- and yellow-in-the-dark strains, 15 mM Na-acetate, added to synchronized cells in the dark, induces an increase in cabII-1 mRNA abundance with a temporal accumulation pattern very similar to that induced by continuous white light. We suggest that by providing an energy source, acetate stimulates cellular growth, cell cycle progression, and increased cabII-1 mRNA abundance. Interestingly, in cells exposed to light, acetate inhibits the light-induced increase in cabII-1 mRNA abundance by a mechanism which is not yet understood.

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“…light and acetate) are present simultaneously. The light-regulated expression of plastid genes is well documented by numerous reports (for reviews, see Mullet, 1988;Taylor, 1989;Herrmann et al, 1992), whereas only a limited number of papers are concerned with the regulatory role of metabolites on the expression of plastid genes (Boege et al, 1981;Westhoff and Zetsche, 1981;Pineau, 1982;Steinmiiller and Zetsche, 1984;Monroy and Schwartzbach, 1984;Steinbiss and Zetsche, 1986;Sheen, 1990).…”

Section: Schaftmentioning

confidence: 99%

Opposite Regulation of the Copy Number and the Expression of Plastid and Mitochondrial Genes by Light and Acetate in the Green Flagellate Chlorogonium

Kroymann¹,

Schneider²,

Zetsche³

1995

Plant Physiol.

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In the unicellular green alga Cblorogonium elongatum (Chlamydomonadaceae), the formation of both the photosynthetic and the respiratory apparatus is under the control of light and acetate. The unicellular green alga Cklovogonium elongatum (Chlamydomonadaceae) is able to quickly adapt its metabolism to very different nutritional conditions. The organism can be cultured either in a simple mineral salt medium with light as energy and CO, as the carbon source (autotrophic conditions), in the dark with acetate as energy and carbon source (heterotrophic conditions), or in the presence of light, acetate, and CO, (mixotrophic conditions).Since the energy charge of a plant cell depends mainly on the activities of the photosynthetic and the respiratory apparatus, the question emerges whether the adaptation to such different energy sources occurs already at the level of the genes responsible for the formation of these apparatus. In this context one has to distinguish between genes located in the organelles and genes located in the nucleus coding for organelle proteins. Furthermore, it is of great interest to examine whether a coordination of the expression of the genes responsible for the formation of the photosynthetic and the respiratory apparatus, respectively,

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Photo- and Metabolite Regulation of the Synthesis of Ribulose Bisphosphate Carboxylase/Oxygenase and the Phycobiliproteins in the Alga Cyanidium caldarium

Cited by 32 publications

References 15 publications

Ribulose bisphosphate carboxylase gene expression in subtropical marine phytoplankton populations

Ribulose bisphosphate carboxylase gene expression in subtropical marine phytoplankton populations

Expression of a gene for a light-harvesting chlorophyll a/b-binding protein in Chlamydomonas reinhardtii: effect of light and acetate

Opposite Regulation of the Copy Number and the Expression of Plastid and Mitochondrial Genes by Light and Acetate in the Green Flagellate Chlorogonium

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