Phosphorylation of Theanine With Cyclo-Triphosphate in Aqueous Solution

Abstract

Phosphorylation of theanine (Thea) has been achieved using inorganic cyclo-triphosphate (P 3m ) in aqueous solution. The optimum condition for the phosphorylation of Thea with P 3m is Thea : P 3m = 2.0 M : 0.5 M, pH 11 and 10 ºC. N 2 -(Phosphono)theanine was synthesized with the yield of more than 90 % and then remained constant after 40 d without hydrolysis of the monophosphate derivative. The phosphorylated product of Thea has a N-P bond in the molecule. The reaction mechanism of Thea with P 3m was discussed.

INTRODUCTION OF PHOSPHATE GROUP INTO β-ARBUTIN BY CYCLO-TRIPHOSPHATE

“…P 3m has been used for phosphorylating agent since Quimby and Flautt 9 produced amidotriphosphate in 1958. Because of its unique structure, biologically important compounds having an amino or a hydroxyl group can be phosphorylation by P 3m 10,11 . Also, the phosphorylation reactions were proceeded in aqueous solution by one-step.…”