Phosphorylation of the DNA repair scaffold SLX4 drives folding of the SAP domain and activation of the MUS81-EME1 endonuclease

Payliss, Brandon J.; Tse, Ying Wah E; Reichheld, Sean E.; Lemak, Alexander; Yun, Hwa Young; Houliston, Scott; Patel, Ayushi; Arrowsmith, C.H.; Sharpe, Simon; Wyatt, Haley D.M.

doi:10.1016/j.celrep.2022.111537

Cited by 13 publications

(22 citation statements)

References 51 publications

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“…Specific recognition sequences can also be included in the substrate design (e.g., transcription factor binding motifs or restriction enzyme target sequences). Depending on the research aims, the DNA substrates can be incubated with the protein of interest in its purified 1 , 2 , 3 , 4 , 5 or partially purified form. 6 , 7 Additionally, users have the option to assay DNA binding or cleavage.…”

Section: Before You Beginmentioning

confidence: 99%

“…Traditionally, DNA substrates are prepared by annealing complementary oligonucleotides, one of which is 5′-end-labeled with 32 P. 1 , 3 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 However, there are caveats to using radioisotopes, most of which arise from the potential health hazards of radioactivity. For example, users require radiation training and a dedicated workspace and equipment for the safe handling, storage, and disposal of radioactive materials.…”

Section: Before You Beginmentioning

confidence: 99%

“… 1 FAM-ACGCTGCCGAATTCTACCAGTGCC TTGCTAGGACATCTTTGCCCACCTGCAG GTTCACCC Oligo 2 Payliss and Tse et al. 1 GGGTGAACCTGCAGGTGGGCAAAGAT GTCCATCTGTTGTAATCGTCAAGCTTT ATGCCGT Oligo 3 Payliss and Tse et al. 1 ACGGCATAAAGCTTGACGATTACAAC AGATCATGGAGCTGTCTAGAGGATC CGACTATCG Oligo 4 Payliss and Tse et al.…”

Section: Key Resources Tablementioning

confidence: 99%

“… 1 GGGTGAACCTGCAGGTGGGCAAAGAT GTCCATCTGTTGTAATCGTCAAGCTTT ATGCCGT Oligo 3 Payliss and Tse et al. 1 ACGGCATAAAGCTTGACGATTACAAC AGATCATGGAGCTGTCTAGAGGATC CGACTATCG Oligo 4 Payliss and Tse et al. 1 CGATAGTCGGATCCTCTAGACAGCTC CATGTAGCAAGGCACTGGTAGAATTC GGCAGCGT Oligo 2.5 Payliss and Tse et al.…”

Section: Key Resources Tablementioning

confidence: 99%

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Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

Tse¹,

Yun²,

Wyatt³

2023

STAR Protocols

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Section: Before You Beginmentioning

confidence: 99%

Section: Before You Beginmentioning

confidence: 99%

Section: Key Resources Tablementioning

confidence: 99%

Section: Key Resources Tablementioning

confidence: 99%

See 2 more Smart Citations

Annealing and purification of fluorescently labeled DNA substrates for in vitro assays

Tse¹,

Yun²,

Wyatt³

2023

STAR Protocols

Self Cite

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“…In contrast to S. cerevisiae , fission yeast Eme1 phosphorylation does not depend on Polo kinase. In human cells, CDK stimulates MUS81-EME1 resolvase activity by promoting the interaction with SLX1-SLX4 SSE ( Wyatt et al, 2013 ; Payliss et al, 2022 ). However, the relevance in meiosis of these phospho-regulations have not been studied.…”

Section: Meiosis-specific Events Regulated By Cyclins and Cdksmentioning

confidence: 99%

Cyclins and CDKs in the regulation of meiosis-specific events

Palacios-Blanco

Martı́n-Castellanos

2022

Front. Cell Dev. Biol.

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How eukaryotic cells control their duplication is a fascinating example of how a biological system self-organizes specific activities to temporally order cellular events. During cell cycle progression, the cellular level of CDK (Cyclin-Dependent Kinase) activity temporally orders the different cell cycle phases, ensuring that DNA replication occurs prior to segregation into two daughter cells. CDK activity requires the binding of a regulatory subunit (cyclin) to the core kinase, and both CDKs and cyclins are well conserved throughout evolution from yeast to humans. As key regulators, they coordinate cell cycle progression with metabolism, DNA damage, and cell differentiation. In meiosis, the special cell division that ensures the transmission of genetic information from one generation to the next, cyclins and CDKs have acquired novel functions to coordinate meiosis-specific events such as chromosome architecture, recombination, and synapsis. Interestingly, meiosis-specific cyclins and CDKs are common in evolution, some cyclins seem to have evolved to acquire CDK-independent functions, and even some CDKs associate with a non-cyclin partner. We will review the functions of these key regulators in meiosis where variation has specially flourished.

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