Phosphorylation of the auxin signaling transcriptional repressor IAA15 by MPKs is required for the suppression of root development under drought stress in Arabidopsis

Kim, Sun Ho; Bahk, Sunghwa; Nguyen, Nhan; Pham, Minh Le Anh; Kadam, Ulhas Sopanrao; Hong, Jong Chan; Chung, Woo Sik

doi:10.1093/nar/gkac798

Cited by 16 publications

(6 citation statements)

References 85 publications

(81 reference statements)

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“…It is also evident that the presence of KRP3 in a checked volume in dividing cells is the decisive factor for cell division rate. MAP Kinase signaling had diverse effects on its substrates due to phosphorylation, which includes modulating localization, gain or loss of function, or even affecting stability (Bhagat et al, 2022b;Kim et al, 2022). Some KRP proteins contain an NLS signal and get localized into the nucleus (Boruc et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of KRP3, a KIP-related protein by MPK3 modulates rice tiller and seed number by fine-tuning cell division

Banerjee,

Jonwal,

Pal

et al. 2024

Preprint

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During the cell cycle process, multiple inhibitors function as checkpoint regulators ensuring an impeccable duplication of genetic material. Kip-Related Proteins (KRPs) are a group of plant-specific cell cycle inhibitors and are known to regulate plant architecture and yield by differentially regulating cell division. KRPs, though functionally conserved, are dynamic in their amino acid composition. Interestingly, some KRPs are specific to dicots, and some are for monocots. In this study, we have identified that the presence of KRP3 and KRP6 is specific to the Poaceae family of monocots. An in-depth study of KRP3 showed a strict regulation of its expression in actively dividing cells during the G1-S phase progression of cell division. Our study identified KRP3 as a phosphorylation target of MPK3. The phosphorylation enhances KRP3 protein stability, which leads to strong inhibition of cell proliferation. By generating knock-out lines of krp3, mpk3 and double knock-out krp3mpk3, our study demonstrated that the MPK3-KRP3 module regulates rice root and shoot development as well as tiller and seed numbers. It was observed that KRP3 regulate the rate of cell division in a dose-dependent manner. The study, in a nutshell, establishes the role of KRP3 as an important regulator of rice vigor and yield.

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Section: Discussionmentioning

confidence: 99%

Phosphorylation of KRP3, a KIP-related protein by MPK3 modulates rice tiller and seed number by fine-tuning cell division

Banerjee,

Jonwal,

Pal

et al. 2024

Preprint

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“…MAPK, as a kinase, is mainly involved in plant stress response by phosphorylating its substrates, including TFs. In Arabidopsis, phosphorylation of MYB15 by MAPK6 is required for freezing tolerance, phosphorylation of IAA15 by MPK3 and MPK6 is required for the suppression of root development under drought stress (Kim et al, 2017(Kim et al, , 2022. ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize (Xiang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

A B‐box transcription factor OsBBX17 regulates saline‐alkaline tolerance through the MAPK cascade pathway in rice

Shen,

Xu,

Chen

et al. 2023

New Phytologist

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Summary Rice OsBBX17 encodes a B‐box zinc finger transcription factor in which the N‐terminal B‐box structural domain interacts with OsMPK1. In addition, it directly binds to the G‐box of OsHAK2 and OsHAK7 promoters and represses their transcription. Under saline‐alkaline conditions, the expression of OsBBX17 was inhibited. Meanwhile, activation of the OsMPK1‐mediated mitogen‐activated protein kinase cascade pathway caused OsMPK1 to interact with OsBBX17 and phosphorylate OsBBX17 at the Thr‐95 site. It reduced OsBBX17 DNA‐binding activity and enhanced saline‐alkaline tolerance by deregulating transcriptional repression of OsHAK2 and OsHAK7. Genetic assays showed that the osbbx17‐KO had an excellent saline‐alkaline tolerance, whereas the opposite was in OsBBX17‐OE. In addition, overexpression of OsMPK1 significantly improved saline‐alkaline tolerance, but knockout of OsMPK1 caused an increased sensitivity. Further overexpression of OsBBX17 in the osmpk1‐KO caused extreme saline‐alkaline sensitivity, even a quick death. OsBBX17 was validated in saline‐alkaline tolerance from two independent aspects, transcriptional level and post‐translational protein modification, unveiling a mechanistic framework by which OsMPK1‐mediated phosphorylation of OsBBX17 regulates the transcription of OsHAK2 and OsHAK7 to enhance the Na+/K+ homeostasis, which partially explains light on the molecular mechanisms of rice responds to saline‐alkaline stress via B‐box transcription factors for the genetic engineering of saline‐alkaline tolerant crops.

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“…In reaction to stress, MKK4 and MKK5 overexpression has been shown to promote MPK3 and MPK6 expression ( Asai et al., 2002 ). MPK3 and MPK6 effectively phosphorylated IAA15 at the Ser-2 and Thr-28 residues ( Kim et al., 2022 ), decreasing lateral root growth due to increased IAA15 accumulation, leading to inhibit the formation of lateral roots in response to drought. Moreover, increased MKK5 expression caused by MKK2 overexpression has been shown to be effective in triggering MPK3 and MPK6 in response to stresses ( Asai et al., 2002 ), resulting in the significance of MKK2 as a transcriptional regulator gene that makes plants more resistant to salt and cold ( Teige et al., 2004 ).…”

Section: Discussionmentioning

confidence: 99%

CRISPR-mediated genome editing in poplar issued by efficient transformation

et al. 2023

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BackgroundCRISPR has been increasingly used for plant genetic improvements because of its high efficiency and precision. Recently, the authors have reported the possibility of homology-directed repair (HDR) using CRISPR/Cas9 through woody plants such as poplar. HDR often replaces nucleotides with one donor DNA template (DDT), including homologous sequences.MethodsCRISPR–Cas9 was recruited, and three variables, Agrobacteria inoculator concentration, pDDT/pgRNA ratio, and homologous arm length, were designed to integrate nptII and 2XCamV 35S into the MKK2 promoter zone.ResultsHere, we showed that recovered poplars on kanamycin-supplemented media exhibited enhanced expression of MKK2 affected by the precise integration of 2XcamV 35S and nptII, improving biochemical and phenotypic properties. Our findings confirmed that Agrobacterium inoculator OD600 = 2.5, increased DDT numbers during cell division to 4:1 pDDT/pgRNA, and optimized homologous arms 700 bp caused efficient HDR and increased MKK2 expression.ConclusionEfficient transformations resulted from optimized variables, directly affecting the HDR efficiency through woody plants such as poplar.

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Phosphorylation of the auxin signaling transcriptional repressor IAA15 by MPKs is required for the suppression of root development under drought stress in Arabidopsis

Cited by 16 publications

References 85 publications

Phosphorylation of KRP3, a KIP-related protein by MPK3 modulates rice tiller and seed number by fine-tuning cell division

Phosphorylation of KRP3, a KIP-related protein by MPK3 modulates rice tiller and seed number by fine-tuning cell division

A B‐box transcription factor OsBBX17 regulates saline‐alkaline tolerance through the MAPK cascade pathway in rice

CRISPR-mediated genome editing in poplar issued by efficient transformation

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