Phosphorylation of dephosphorylated tRNA and oligonucleotides by polynucleotide kinase

Hänggi, Urs J.; Streeck, Rolf E.; Voigt, Hans-Peter; Zachau, Hans G.

doi:10.1016/0005-2787(70)90527-7

Cited by 35 publications

(10 citation statements)

References 25 publications

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“…Aminoacylation capacity of E. coli dephosphorylated tRNAs, specific for serine, methionine, phenylalanine and histidine, have already been tested. Except tRNA His [14], all tested tRNAs retain their capacity to be aminoacylated [15–17].…”

Section: Resultsmentioning

confidence: 99%

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

et al. 1998

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A limitation for a universal use of T7 RNA polymerase for in vitro tRNA transcription lies in the nature of the often unfavorable 5P-terminal sequence of the gene to be transcribed. To overcome this drawback, a hammerhead ribozyme sequence was introduced between a strong T7 RNA polymerase promoter and the tDNA sequence. Transcription of this construct gives rise to a`transzyme' molecule, the autocatalytic activity of which liberates a 5P-OH tRNA transcript starting with the proper nucleotide. The method was optimized for transcription of yeast tRNA yr , starting with 5P-C I , and operates as well for yeast tRNA esp with 5P-U I . Although the tRNAs produced by the transzyme method are not phosphorylated, they are fully active in aminoacylation with k t and K m parameters quasi identical to those of their phosphorylated counterparts.z 1998 Federation of European Biochemical Societies.

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Section: Resultsmentioning

confidence: 99%

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

et al. 1998

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“…AT3 (or the corresponding purine in certain tRNA sequences) may be sterically protected by the hU loop against chemical modifications [5] ; likewise the ability of the 5' end to be enzymatically phosphory lated will depend on the position of the hU loop [26].…”

Section: Correlation Of Experimentai Results With the Theoretanda1 Modelmentioning

confidence: 99%

A dynamic molecular model for transfer RNA

Danchin

1971

FEBS Letters

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“…I n tRNAPhe the acceptor stem was protected in both the native and the denatured form. The denaturation had little effect on the conformation of the 5'-end also according to phosphorylation experiments with polynucleotide kinase [27]. Nu- Fig.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Native and Denatured Conformations of tRNA^Ser and tRNA^Phe from Yeast

Streeck¹,

Zachau²

1972

European Journal of Biochemistry

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Previous work on the partial digestion of the native and denatured forms of tRNASer and tRNAPhe from yeast with TI-RNAase was extended. Detailed studies with pancreatic and T2-RNAase and a few experiments with sheep kidney nuclease and acid RNAase from hog spleen revealed characteristically different fragmentation patterns for the native and denatured forms of the tRNAs. Apparently it is mainly the miniloop and dihydrouridine regions which are involved in the conformational changes responsible for denaturation.The rate constants, activation enthalpies, and activation entropies of the renaturation process were calculated from amino acid acceptance data.When the changes in hyperchromicity a t 260 nm of the denatured tRNAs were followed as a function of time, a process was detected which in tRNAPhe is 5-10 times and in tRNAser 10-20 times faster than the renaturation as measured by acceptor activity. The whole net change in absorption, possibly representing the total net change in base stacking, occurs during the fast, process. The activation enthalpies and activation entropies are different for the fast and t,he over-all processes.

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Phosphorylation of dephosphorylated tRNA and oligonucleotides by polynucleotide kinase

Cited by 35 publications

References 25 publications

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

A dynamic molecular model for transfer RNA

Characterization of the Native and Denatured Conformations of tRNA^Ser and tRNA^Phe from Yeast

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Phosphorylation of dephosphorylated tRNA and oligonucleotides by polynucleotide kinase

Cited by 35 publications

References 25 publications

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

Ribozyme processed tRNA transcripts with unfriendly internal promoter for T7 RNA polymerase: production and activity

A dynamic molecular model for transfer RNA

Characterization of the Native and Denatured Conformations of tRNASer and tRNAPhe from Yeast

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Characterization of the Native and Denatured Conformations of tRNA^Ser and tRNA^Phe from Yeast