Phosphorylation of ATG18a by BAK1 suppresses autophagy and attenuates plant resistance against necrotrophic pathogens

Bao, Zhenmin; Shao, Lu; Wang, Jiali; Zhang, Yan; Guo, Xiaoshuang; Peng, Yujiao; Cao, Yangrong; Lai, Zhibing

doi:10.1080/15548627.2020.1810426

Cited by 44 publications

(31 citation statements)

References 73 publications

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“…Atg18 in the yeast Pichia pastoris was shown to be phosphorylated, and phosphorylation enhanced its binding affinity to PtdIns(3)P (57). However, this modification in P. pastoris does not affect autophagy activity, while phosphorylation of Arabidopsis ATG18a inhibits its function in autophagy (58). Therefore, posttranslational regulation of ATG18a must be finely controlled and probably triggered depending on the stress to which the plant is subjected.…”

Section: Discussionmentioning

confidence: 99%

Persulfidation of ATG18a regulates autophagy under ER stress inArabidopsis

Aroca

Yruela²,

Gotor

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Hydrogen sulfide (H2S) is an endogenously generated gaseous signaling molecule, which recently has been implicated in autophagy regulation in both plants and mammals through persulfidation of specific targets. Persulfidation has been suggested as the molecular mechanism through which sulfide regulates autophagy in plant cells. ATG18a is a core autophagy component that is required for bulk autophagy and also for reticulophagy during endoplasmic reticulum (ER) stress. In this research, we revealed the role of sulfide in plant ER stress responses as a negative regulator of autophagy. We demonstrate that sulfide regulates ATG18a phospholipid-binding activity by reversible persulfidation at Cys103, and that this modification activates ATG18a binding capacity to specific phospholipids in a reversible manner. Our findings strongly suggest that persulfidation of ATG18a at C103 regulates autophagy under ER stress, and that the impairment of persulfidation affects both the number and size of autophagosomes.

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Section: Discussionmentioning

confidence: 99%

Persulfidation of ATG18a regulates autophagy under ER stress inArabidopsis

Aroca

Yruela²,

Gotor

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…10 , Additional file: Table S 5 ). Previous studies have shown that some key PRRs in PTI such as brassinosteroid insensitive 1-associated kinase 1 (BAK1) [ 39 ], FLS2 [ 40 ] and chitin elicitor receptor kinase (CERK) [ 41 ], play an important role in plant resistance to pathogens. Notably, one CERK and two FLS2 genes exhibited differently between two genotypes.…”

Section: Discussionmentioning

confidence: 99%

The mechanism of sesame resistance against Macrophomina phaseolina was revealed via a comparison of transcriptomes of resistant and susceptible sesame genotypes

Yan

Liu

et al. 2021

BMC Plant Biol

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Background Sesame (Sesamum indicum) charcoal rot, a destructive fungal disease caused by Macrophomina phaseolina (Tassi) Goid (MP), is a great threat to the yield and quality of sesame. However, there is a lack of information about the gene-for-gene relationship between sesame and MP, and the molecular mechanism behind the interaction is not yet clear. The aim of this study was to interpret the molecular mechanism of sesame resistance against MP in disease-resistant (DR) and disease-susceptible (DS) genotypes based on transcriptomics. This is the first report of the interaction between sesame and MP using this method. Results A set of core genes that response to MP were revealed by comparative transcriptomics and they were preferentially associated with GO terms such as ribosome-related processes, fruit ripening and regulation of jasmonic acid mediated signalling pathway. It is also exhibited that translational mechanism and transcriptional mechanism could co-activate in DR so that it can initiate the immunity to MP more rapidly. According to weighted gene co-expression network analysis (WGCNA) of differentially expressed gene sets between two genotypes, we found that leucine-rich repeat receptor-like kinase (LRR-RLK) proteins may assume an important job in sesame resistance against MP. Notably, compared with DS, most key genes were induced in DR such as pattern recognition receptors (PRRs) and resistance genes, indicating that DR initiated stronger pattern-triggered immunity (PTI) and effector-triggered immunity (ETI). Finally, the study showed that JA/ET and SA signalling pathways all play an important role in sesame resistance to MP. Conclusions The defence response to MP of sesame, a complex bioprocess involving many phytohormones and disease resistance-related genes, was illustrated at the transcriptional level in our investigation. The findings shed more light on further understanding of different responses to MP in resistant and susceptible sesame.

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“…While atg2 mutants displayed less HR-PCD and ATG4, ATG5 inhibited the occurrence of HR-PCD, ATG6 antisense plants displayed enhanced HR-PCD during pathogen infection [53][54][55][56][57][58][59]61]. A recent study reported that phosphorylation modification of ATG18a suppressed autophagosome formation during pathogen infection, resulting in compromised plant resistance, which provides evidence for the involvement of autophagy in plant immune regulation [62]. Here, we summarize the interaction between bacteria, fungal effectors, and ATGs as well as the role of autophagy in HR-PCD and resistance regulation (Table 1).…”

Section: Atgs Involved In Plant Resistance To Pathogensmentioning

confidence: 97%

Research Progress of ATGs Involved in Plant Immunity and NPR1 Metabolism

Huang

Zhang

Chen

2021

IJMS

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Autophagy is an important pathway of degrading excess and abnormal proteins and organelles through their engulfment into autophagosomes that subsequently fuse with the vacuole. Autophagy-related genes (ATGs) are essential for the formation of autophagosomes. To date, about 35 ATGs have been identified in Arabidopsis, which are involved in the occurrence and regulation of autophagy. Among these, 17 proteins are related to resistance against plant pathogens. The transcription coactivator non-expressor of pathogenesis-related genes 1 (NPR1) is involved in innate immunity and acquired resistance in plants, which regulates most salicylic acid (SA)-responsive genes. This paper mainly summarizes the role of ATGs and NPR1 in plant immunity and the advancement of research on ATGs in NPR1 metabolism, providing a new idea for exploring the relationship between ATGs and NPR1.

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Phosphorylation of ATG18a by BAK1 suppresses autophagy and attenuates plant resistance against necrotrophic pathogens

Cited by 44 publications

References 73 publications

Persulfidation of ATG18a regulates autophagy under ER stress inArabidopsis

Persulfidation of ATG18a regulates autophagy under ER stress inArabidopsis

The mechanism of sesame resistance against Macrophomina phaseolina was revealed via a comparison of transcriptomes of resistant and susceptible sesame genotypes

Research Progress of ATGs Involved in Plant Immunity and NPR1 Metabolism

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