2018
DOI: 10.1186/s12870-018-1364-8
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Phosphorylation of Arabidopsis SINA2 by CDKG1 affects its ubiquitin ligase activity

Abstract: BackgroundSEVEN IN ABSENTIA (SINA) is a RING domain-containing ubiquitin ligase involved in Drosophila eye formation. SINA-like proteins in plants are involved in several signaling pathways. Of the 18 SINA-like proteins identified in Arabidopsis, SEVEN IN ABSENTIA 2 (SINA2) lacks a canonical RING domain and is thought to lack ubiquitin ligase activity.ResultsOur results show that SINA2 has E3 ligase activity in vitro, raising the possibility that a modified B-box domain may compensate for its lack of a RING do… Show more

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Cited by 12 publications
(10 citation statements)
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References 50 publications
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“…In fact, OsDIRP1 is predicted to possess at least six putative phosphorylation sites (Supplementary Figure S6). Phosphorylation was shown to be required to activate Arabidopsis ICE1 (Inducer of CBF Expression), SINA2 (SEVER IN ABSENTIA), and RFP34/CHYR1 (RING ZINC-FINGER PROTEIN34/CHY ZINC-FINGER AND RING PROTEIN 1) E3 ligases as well as the rice OsPUB15 E3 Ub ligase (Ding Y. et al, 2015; Ding S. et al, 2015; Wang et al, 2015; Chen et al, 2018). Second, OsDIRP1 may not be a bona fide E3 ligase, but instead may play an unidentified role in response to environmental stimuli.…”
Section: Discussionmentioning
confidence: 99%
“…In fact, OsDIRP1 is predicted to possess at least six putative phosphorylation sites (Supplementary Figure S6). Phosphorylation was shown to be required to activate Arabidopsis ICE1 (Inducer of CBF Expression), SINA2 (SEVER IN ABSENTIA), and RFP34/CHYR1 (RING ZINC-FINGER PROTEIN34/CHY ZINC-FINGER AND RING PROTEIN 1) E3 ligases as well as the rice OsPUB15 E3 Ub ligase (Ding Y. et al, 2015; Ding S. et al, 2015; Wang et al, 2015; Chen et al, 2018). Second, OsDIRP1 may not be a bona fide E3 ligase, but instead may play an unidentified role in response to environmental stimuli.…”
Section: Discussionmentioning
confidence: 99%
“…The suggestion that the CDKG1 gene has arisen from a retrotransposition event from the CDKG2 gene in the Arabidopsis lineage after the split from other Brassicaceae ( Zhang et al, 2005 ; Beilstein et al, 2010 ) has raised questions related to their function and why both genes have been retained. Even though CDKG1 and CDKG2 proteins share 64% amino acid similarity, we and others have shown that the two kinases perform different functions ( Huang et al, 2013 ; Zheng et al, 2014 ; Ma et al, 2015 ; Cavallari et al, 2018 ; Chen et al, 2018 ; Nibau et al, 2020a , b ). On the other hand, we were not able to recover double cdkg1-1cdkg2-1 mutants despite both single mutants showing only mild growth phenotypes.…”
Section: Discussionmentioning
confidence: 87%
“…In A. thaliana , it is highly and mostly expressed during pollen development (The Arabidopsis Information Resource [ 27 ];). Its activity is modulated by CDKG1 [ 28 ], which is involved in thermal-sensitive male meiosis [ 29 ].…”
Section: Discussionmentioning
confidence: 99%