A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis

Nibau, Cândida; Dadarou, Despoina; Kargios, Nestoras; Mallioura, Areti; Fernández-Fuentes, Narcís; Cavallari, Nicola; Doonan, John

doi:10.3389/fpls.2020.586870

Cited by 7 publications

(5 citation statements)

References 86 publications

(126 reference statements)

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“…A. thaliana CDKG1 protein has a critical role in maintaining pollen fertility and yield at high ambient temperatures (Zheng et al ., 2014). CDKG1 is reported to function through the phosphorylation and/or regulation of the splicing of proteins involved in the early stages of meiotic division, possibly the synaptonemal complex protein 1 (SYP1) (Nibau et al ., 2020 a ). CDKG2 and the associated CYCL1 appear to act as a negative regulator of flowering timing (Ma et al ., 2015; Nibau et al ., 2020 b ), since in cdkg2‐1 as well as in the double cdkg2‐1;cycL1‐1 mutant lines, FLM‐δ was up‐regulated but FLM‐β down‐regulated, resulting in an early flowering phenotype in a temperature‐dependent manner, under both LD and SD conditions (Nibau et al ., 2020 b ).…”

Section: Role Of Phosphorylation In Signal Transduction Of Floweringmentioning

confidence: 99%

Phosphorylation‐mediated signalling in flowering: prospects and retrospects of phosphoproteomics in crops

2021

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Protein phosphorylation is a major post-translational modification, regulating protein function, stability, and subcellular localization. To date, annotated phosphorylation data are available mainly for model organisms and humans, despite the economic importance of crop species and their large kinomes. Our understanding of the phosphoregulation of flowering in relation to the biology and interaction between the pollen and pistil is still significantly lagging, limiting our knowledge on kinase signalling and its potential applications to crop production. To address this gap, we bring together relevant literature that were previously disconnected to present an overview of the roles of phosphoproteomic signalling pathways in modulating molecular and cellular regulation within specific tissues at different morphological stages of flowering. This review is intended to stimulate research, with the potential to increase crop productivity by providing a platform for novel molecular tools.

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Section: Role Of Phosphorylation In Signal Transduction Of Floweringmentioning

confidence: 99%

Phosphorylation‐mediated signalling in flowering: prospects and retrospects of phosphoproteomics in crops

2021

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“…In agreement, the long isoform could restore the cdkg1 mutant defect in terms of the male sterility at high temperature, while the short isoform could not. 80 The above mentioned results indicate CDKG1L and CDKG1S is regulated by temperature to antagonistically control pollen development. However, on the other hand, CDKG1L and CDKG1S synergistically mediate the correct splicing of U2AF65A in response to fluctuating ambient temperatures.…”

Section: Involvement Of Alternative Splicing In Plant Development and Stress Responsementioning

confidence: 92%

“…Besides the roles for AS of MP in ovule, AS of CYCLIN-DEPENDENT KINASE G1 ( CDKG1 ) is implicated in pollen development in response to ambient temperature. 80 Two AS events resulted from CDKG1 via the selective intron mainly encodes two protein isoforms CDKG1L (long) and CDKG1S (short). The long isoform exclusively localized in the nuclear, but the short isoform lacking two out of four SR domains and NLS localized in both the nuclear and cytoplasm.…”

Section: Involvement Of Alternative Splicing In Plant Development and Stress Responsementioning

confidence: 99%

Normal, novel or none: versatile regulation from alternative splicing

Liu

Tang

Liu

et al. 2021

Plant Signaling & Behavior

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Pre-mRNA splicing is a vital step in the posttranscriptional regulation of gene expression. Splicing is catalyzed by the spliceosome, a multidalton RNA–protein complex, through two successive transesterifications to yield mature mRNAs. In Arabidopsis, more than 61% of all transcripts from intron-containing genes are alternatively spliced, thereby resulting in transcriptome and subsequent proteome diversities for cellular processes. Moreover, it is estimated that more alternative splicing (AS) events induced by adverse stimuli occur to confer stress tolerance. Recently, increasing AS variants encoding normal or novel proteins, or degraded by nonsense-mediated decay (NMD) and their corresponding splicing factors or regulators acting at the posttranscriptional level have been functionally characterized. This review comprehensively summarizes and highlights the advances in our understanding of the biological functions and underlying mechanisms of AS events and their regulators in Arabidopsis and provides prospects for further research on AS in crops.

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“…However, CDKG;2 acts in different biological processes than CDKG;1. CDKG;1 is essential for synapsis and recombination during male meiosis [ 71 , 72 ], while CDKG;2 is involved in flowering and response to heat and salt stresses [ 68 , 69 , 73 ]. Interestingly, the CDKG;1 transcript level decreased from G1/G0 to S and G2, although CDKG;2 expression stayed almost the same during cell cycle progression [ 13 ].…”

Section: Functions Of Cdks and Ctd Phosphatases Unique To Arabidopsismentioning

confidence: 99%

Cyclin-Dependent Kinases and CTD Phosphatases in Cell Cycle Transcriptional Control: Conservation across Eukaryotic Kingdoms and Uniqueness to Plants

Zheng

2022

Cells

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Cell cycle control is vital for cell proliferation in all eukaryotic organisms. The entire cell cycle can be conceptually separated into four distinct phases, Gap 1 (G1), DNA synthesis (S), G2, and mitosis (M), which progress sequentially. The precise control of transcription, in particular, at the G1 to S and G2 to M transitions, is crucial for the synthesis of many phase-specific proteins, to ensure orderly progression throughout the cell cycle. This mini-review highlights highly conserved transcriptional regulators that are shared in budding yeast (Saccharomyces cerevisiae), Arabidopsis thaliana model plant, and humans, which have been separated for more than a billion years of evolution. These include structurally and/or functionally conserved regulators cyclin-dependent kinases (CDKs), RNA polymerase II C-terminal domain (CTD) phosphatases, and the classical versus shortcut models of Pol II transcriptional control. A few of CDKs and CTD phosphatases counteract to control the Pol II CTD Ser phosphorylation codes and are considered critical regulators of Pol II transcriptional process from initiation to elongation and termination. The functions of plant-unique CDKs and CTD phosphatases in relation to cell division are also briefly summarized. Future studies towards testing a cooperative transcriptional mechanism, which is proposed here and involves sequence-specific transcription factors and the shortcut model of Pol II CTD code modulation, across the three eukaryotic kingdoms will reveal how individual organisms achieve the most productive, large-scale transcription of phase-specific genes required for orderly progression throughout the entire cell cycle.

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A Functional Kinase Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis

Cited by 7 publications

References 86 publications

Phosphorylation‐mediated signalling in flowering: prospects and retrospects of phosphoproteomics in crops

Phosphorylation‐mediated signalling in flowering: prospects and retrospects of phosphoproteomics in crops

Normal, novel or none: versatile regulation from alternative splicing

Cyclin-Dependent Kinases and CTD Phosphatases in Cell Cycle Transcriptional Control: Conservation across Eukaryotic Kingdoms and Uniqueness to Plants

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