Phosphorylated modification and in vitro antioxidant activity of Radix Hedysari polysaccharide

Wei, Dongfeng; Chen, Weidong; Wei, Yanxia; Zhang, Lifeng

doi:10.1007/s10719-012-9377-2

Cited by 28 publications

(19 citation statements)

References 20 publications

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“…The RHP was purified by the Institute of Combined Traditional Chinese and Western Medicine, School of Basic Medical Science at Lanzhou University (Lanzhou, China) (6,9). The polysaccharide content was 97.3%.…”

Section: Methodsmentioning

confidence: 99%

“…Radix Hedysari polysaccharide (RHP) is the main bioactive ingredient of RH, and possesses several pharmacological activities, including antitumor, anti-inflammatory, antioxidant and immunomodulatory effects (6,7). Previous studies have also suggested that RHP may have hypoglycemic and hypolipidemic properties and may improve insulin resistance (8), however, the effect of RHP on NAFLD remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

Sun

Wang

Duan

et al. 2014

Molecular Medicine Reports

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Oxidative stress and excess hepatic lipid accumulation contribute to non-alcoholic fatty liver disease. Radix Hedysari polysaccharides (RHP) have attracted interest due to their antioxidant properties and immunomodulatory effects. However, the effect of RHP on hepatic lipid metabolism remains to be elucidated. In the present study, the response of Sprague-Dawley rat livers to a high-fat diet and RHP treatment was investigated by evaluating body weight, liver histology, hepatic lipid content, adenosine monophosphate-activated protein kinase (AMPK) activity and lipid metabolism gene transcriptional profiles. The present study demonstrated that RHP ameliorated lipid metabolism disorders, regulated hepatic lipid content, improved liver inflammation and damage, activated AMPK via phosphorylation, upregulated peroxisome proliferator-activated receptor α and downregulated the mRNA expression of sterol regulatory element binding protein-1c in rat livers, which reduced lipogenesis and increased lipolysis. Taken together, these results suggested that RHP effectively ameliorates lipid metabolism disorders in rat livers; thus, RHP may be a potential therapeutic agent in the prevention of hepatic steatosis.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

Sun

Wang

Duan

et al. 2014

Molecular Medicine Reports

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“…However, these systems are insufficient to prevent the harm entirely [4]. It is reported that free radicals, including superoxide anion, hydroxyl radical, and hydrogen peroxide can cause pathological damages like cancer disease, diabetes, atherosclerosis, coronary heart disease, and many other diseases associated with aging to the organism, and lead to harmful alterations in foods and pharmaceutical industries [5][6][7]. Therefore, it is urgent to develop antioxidant supplements to help the human body reduce oxidative scratch.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and antioxidant properties of novel inulin derivatives with amino-pyridine group

Zhang

Chen

et al. 2014

International Journal of Biological Macromolecules

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a b s t r a c tA series of novel inulin derivatives were synthesized via reaction of chloracetyl inulin (CAIL) with aminopyridines, including 2-(2-amino-pyridyl)acetyl inulin chloride (2APAIL), 2-(3-amino-pyridyl)acetyl inulin chloride (3APAIL), 2-(4-amino-pyridyl)acetyl inulin chloride (4APAIL), 2-(2,3-diaminopyridyl)acetyl inulin chloride (2,3DAPAIL), and 2-(3,4-diamino-pyridyl)acetyl inulin (3,4DAPAIL). The antioxidant property of the products and 2-pyridylacetyl inulin chloride (PAIL) against hydroxyl radicals (• OH), superoxide radicals (O 2 • ), and DPPH radicals (DPPH • ) were evaluated in vitro, respectively. Results showed that 4APAIL and 3,4DAPAIL exhibited remarkable improvement on scavenging• OH and DPPH• , which can scavenge the radical of • OH completely at 0.4 mg/mL. Besides, the scavenging activity of 2,3DAPAIL to O 2• was excellent among all of the tested samples, reaching 85% at 1.6 mg/mL. These data indicate that all of the inulin derivatives have better antioxidant activities than inulin, and the scavenging effect indices are affected by the number and position of the amino group on pyridine grafted to the inulin derivatives.

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“…In addition, a “C–O–S” bond would give rise to the FT-IR band observed at 810 cm −1 (Figure 2c) [13], indicating that the successful sulfation of lentinan. The phosphorylation of lentinan was also confirmed by FT-IR of P-D. Three new strong absorption peaks appeared in the P-D spectrum at 1720, 1270, and 790 cm −1 , and these were assigned to “C=O” stretching vibration, “P=O” asymmetric stretching, and “C–O–P” symmetric vibrations, respectively (Figure 2d) [14]. These absorptions indicated that the phosphorylated modification of lentinan was successfully performed.…”

Section: Resultsmentioning

confidence: 88%

“…Lentinan was phosphorylated following the procedure described by Wei et al [14]. Lentinan (0.5 g) was suspended in anhydrous N , N -Dimethylformamide (DMF) (20 mL) at room temperature under stirring for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans

Chen

et al. 2014

IJMS

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The chemical properties of β-glucans leading to their inhibition on aflatoxin (AF) production by Aspergillus flavus remain unclear. In this study, structurally modified lentinan derivatives were prepared by carboxymethylation, sulfation, and phosphorylation to explore their inhibition activity to AF synthesis. The results demonstrated that inhibitory activity of lentinan decreased at higher or lower concentrations than 200 μg/mL. Compared with lentinan, the sulphated derivatives only performed a reduced optimal inhibition rate at a higher concentration. The phosphorylated derivatives achieved complete inhibition of AF production at 50 μg/mL, but the inhibitory activity was attenuated with an increase of concentration. The minimum concentration of carboxymethylated derivatives to completely inhibit AF synthesis was the same as that of the original lentinan, whereas their inhibition activity was not reduced at the increasing concentration. RT-PCR analyses were conducted to understand the effects of lentinan and its carboxymethylated derivatives on the transcription of certain genes associated with AF biosynthesis. The results showed that lentinan delayed the transcription of aflQ, whereas its carboxymethylated derivatives promoted the transcriptions of all the tested genes. Our results revealed that some chemical group features apart from the β-bond could play the vital role in the prevention of AF formation by polysaccharide, and highlighted the structural modifications which could promote its practicability in the control of aflatoxin contamination.

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Phosphorylated modification and in vitro antioxidant activity of Radix Hedysari polysaccharide

Cited by 28 publications

References 20 publications

Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non-alcoholic fatty liver disease via adenosine monophosphate-activated protein kinase pathway activation

Synthesis, characterization, and antioxidant properties of novel inulin derivatives with amino-pyridine group

Inhibition of Aflatoxin Synthesis in Aspergillus flavus by Three Structurally Modified Lentinans

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