Phosphorylated Connexin 43 Protein Levels Attenuation in the Retina of Adult Diabetic Male Rats, by Regular Moderate Exercise

Hesari, Farzam Sheikhzadeh; Khajehnasiri, Nazli

doi:10.5455/jppa.20130822091453

Cited by 4 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moderate physical exercise is known to affect phosphorylated Cx-43 protein levels. 26 Experimental animals undergoing moderate aerobic exercise are known to have attenuation of Cx43 phosphorylation, then phospho-Cx43 can modulate gap junction performance. 26,27 Data analysis showed a higher total Cx43 expression (in a total area expression) compared to control group, along with a slightly higher lateral Cx43.…”

Section: Discussionmentioning

confidence: 99%

Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats

Ariningpraja

Santoso

Jusuf

et al. 2021

J. Biomed. Transl. Res.

View full text Add to dashboard Cite

Background: Gap Junction (GJ) plays a role in supporting the heart electricity. Connexin43 (Cx43) as the main protein constituent of GJ in left cardiac ventricle, will increase in number and slightly redistributed to the lateral sides of cardiomyocytes after aerobic exercise in adulthood. The effects of aerobic exercise that begin at childhood are not well known.Objective: This study aims to observe the effect of aerobic exercise which started from childhood on left ventricle Cx43 distribution.Methods: This study was conducted on 28 male Juvenile (4 weeks) and young adult (8 weeks) rats, divided into 7 groups: 1) Juvenile rats undergoing 4 weeks of exercise (E-J4); 2) Control E-J4 (C-J4); 3) Juvenile rats undergoing 8 weeks of exercise (E-J8); 4) Control EJ-8 (C-J8); 5) Juvenile rats undergoing 12 weeks of exercise (E-J12; 6); Young adult rats undergoing 8 weeks of exercise (E-Yo8); 7) Control E-J12 and E-Yo8 (C-JY128). Exercise group will undergo different length of duration, starting from week 4 until 12 weeks. Cx43 was identified by immunohistochemical staining and analyzed with ImageJ software. Comparison was analyzed using independent t-test.Results: Insignificant lower of total Cx43 expression in E-J4 (64200.45 + 4243.676 total area, p >0.05) compared to control. In contrast, a significant higher of total Cx43 expression was observed in EJ-8, EJ-12 and E-Yo8 (80152.95 + 3760.481, p = 0.001; 75596.775 + 3976.333, p = 0,002; 81216.85+ 2475.768, p = 0,000). Slightly higher of lateral Cx43 redistribution occurred in all aerobic exercise, with significant lateralization in E-J8 and E-Yo8.Conclusion: Aerobic exercise increases Cx43 and slightly redistributed to lateral myocytes under normal condition both in juvenile and young adult rats.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats

Ariningpraja

Santoso

Jusuf

et al. 2021

J. Biomed. Transl. Res.

View full text Add to dashboard Cite

show abstract

“…Receptors of these cytokines exist in rat and human testicles, and mRNA of receptors can be seen in Sertoli and Leydig cells and testicular macrophages of all species (De et al 1993;Carneiro et al 2010). Physiologically, these two proteins play an important role in testicular tissue homeostasis, while their high levels are pathological (Hesari and Khajehnasiri 2013), such that serum concentrations of TNF-α and IL-1 may increase up to 2 to 3 times higher than their normal levels in chronic diseases such as diabetes (Lysiak 2004). Thus, in the present study the observed testicular tissue changes in diabetic group are not probably mediated by TNF-α and IL-1 inflammatory factors within 2 months of diabetes.…”

Section: Discussionmentioning

confidence: 99%

“…Considering positive effects of exercise in controlling blood glucose levels, it has been reported that blood glucose level in exercised diabetic animals is significantly lower than those of sedentary diabetic animals. Regular exercise brings blood sugar closer to normal levels (Petersen and Pedersen 2005;Hesari et al 2014).…”

mentioning

confidence: 99%

Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats

Mogaddami

Sheikhzadeh

Hatami

et al. 2018

Endocrine Regulations

Self Cite

View full text Add to dashboard Cite

Objective. Reproductive disorders are one of the complications of diabetes mellitus. Since conflicting results have been obtained from different studies, which examined serum levels of cytokines in patients with diabetes, and considering the fact that the origin of cytokines cannot be accurately determined from their serum changes, attempts were made in the present study to study histological changes and testicular tissue levels of TNF-α and IL-1 in rats treated with exercise. Considering the effects of exercise in reducing blood sugar level and its complications, two types of short-term and long-term regular exercises were also considered to evaluate their effects on male reproductive tissues.Methods. In this study, 60 male rats with the weight range of 250±50 g were used and were randomly divided into six groups (10 rats each). Healthy groups included sedentary control group, and groups treated with two and eight weeks of exercise. Rats with type 1 diabetes (induced by streptozotocin) included sedentary control group, groups treated with two and eight weeks of exercise (six groups). All groups were evaluated in terms of testicular tissue levels of TNF-α and IL-1 using ELISA and the histometry of spermatogonia, primary spermatocytes, Sertoli cells, epithelial thickness, diameter of veins, and thickness of the seminiferous tubule.Results. Histological changes resulting from diabetes, particularly in the diameter of testicular veins and a number of cells, including Sertoli, highlights the important fact that tissue perfusion in patients with diabetes is especially crucial, in a way that exercise proved useful for tissue structures by offsetting this complication. Measurement of the cytokines IL-1 and TNF-α in the current study showed that perfusion problems are more important in diabetic complications than inflammatory factors.Conclusions. The main result of this research is recommendation of investigating the tissue of interest for diagnosis of diabetes complications, measuring inflammatory mediators of tissue rather than evaluating their serum concentrations, and focusing on vascular complications as a major complication of diabetes. Furthermore, regular exercise could help improve the function of reproductive organs in healthy groups and prevent diabetes infertility complications to an acceptable degree in diabetic groups.

show abstract

“…The test was made up of three stages respectively as follows: Ι) In the initial stage, the speed of the treadmill reached from 5 m/min to 35 m/min for 10 min to allow animals to warm up and prepare for exercise; II) In the second step, the speed was 35 m/min for 40 min; and III) In the last stage, in order to the recovery of the rats, the speed of the treadmill reached from 35 m/min to 5 m/min for 10 min. The animals in the control group were placed on the turned off treadmill for 60 min (Hesari et al 2014).…”

Section: Methodsmentioning

confidence: 99%

“…One day after the final exercise treatment, when the rats were not allowed to have a food intake, all the animals were anesthetized by intraperitoneal injection (i.p.) of a combination of ketamine hydrochloride (100 mg/kg) and xylazine hydrochloride (5 mg/kg) (Hesari et al 2014).…”

Section: Methodsmentioning

confidence: 99%

One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats

Khajehnasiri

Khazali

Sheikhzadeh

et al. 2019

Endocrine Regulations

Self Cite

View full text Add to dashboard Cite

Objective. The hypothalamic arcuate nucleus proopiomelanocortin (POMC) and neuropeptide Y (NPY) circuitries are involved in the inhibition and stimulation of the appetite, respectively. The aim of this study was to investigate the effects of one-month lasting high-intensity exercise on the POMC mRNA and NPY mRNA expression in the above-mentioned brain structure and appetite and food intake levels.Methods. Fourteen male Wistar rats (250±50 g) were used and kept in the well-controlled conditions (22±2 °C, 50±5% humidity, and 12 h dark/light cycle) with food and water ad libitum. The rats were divided into two groups (n=7): 1) control group (C, these rats served as controls) and 2) exercised group (RIE, these rats performed a high-intensity exercise for one month (5 days per week) 40 min daily with speed 35 m/min. The total exercise time was 60 min. The body weight and food intake were recorded continuously during the experiments.Results. The results showed relative mRNA expression of POMC and NPY estimated in the hypothalamic arcuate nucleus. There were no significant differences in the NPY and POMC mRNAs expression levels and food intake between C and RIE groups.Conclusions. The present data indicate that one-month regular intensive exercise did not alter the levels of NPY and POMC mRNAs expression (as two important factors in the regulation of appetite) in the hypothalamic arcuate nucleus and food intake suggesting that this type of exercise itself is not an appropriate procedure for the body weight reduction.

show abstract

Phosphorylated Connexin 43 Protein Levels Attenuation in the Retina of Adult Diabetic Male Rats, by Regular Moderate Exercise

Cited by 4 publications

References 28 publications

Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats

Effect of Aerobic Exercise on Left Ventricular Connexin43 Expression and Distribution in Juvenile and Young Adult Rats

Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats

One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats

Contact Info

Product

Resources

About