Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.)

Zhang, Ming; Lv, Dongwen; Ge, Pei; Bian, Yanwei; Chen, Guanxing; Zhu, Guijie; Li, Xiaohui; Yan, Yueming

doi:10.1016/j.jprot.2014.07.010

Cited by 136 publications

(136 citation statements)

References 88 publications

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“…Large-scale phosphoproteomic analyses have been conducted on several plant species, including Arabidopsis thaliana 454647484950, rice4851, Medicago truncatula 5253, soybean ( Glycine max L.)4954, canola ( Brassica napus )49, maize555657 Brachypodium distachyon 58 and wheat2659. However, further research on large-scale mining of the phosphorylation sites in starch biosynthetic enzymes has not been performed.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, we used LC-MS/MS to identify several phosphorylation sites present in GBSS I and SS I2324. Together with the development of phosphoproteomic approaches, TiO 2 enrichment-based large-scale phosphoproteomic analysis has been performed in wheat and Brachypodium distachyon 252627. However, large-scale phosphoproteomic analysis of starch granule-binding proteins in cereal crops has not been performed due to two major limitations.…”

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confidence: 99%

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Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins

Chen

Zhou

Liu

et al. 2016

Sci Rep

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Waxy starch has an important influence on the qualities of breads. Generally, grain weight and yield in waxy wheat (Triticum aestivum L.) are significantly lower than in bread wheat. In this study, we performed the first proteomic and phosphoproteomic analyses of starch granule-binding proteins by comparing the waxy wheat cultivar Shannong 119 and the bread wheat cultivar Nongda 5181. These results indicate that reduced amylose content does not affect amylopectin synthesis, but it causes significant reduction of total starch biosynthesis, grain size, weight and grain yield. Two-dimensional differential in-gel electrophoresis identified 40 differentially expressed protein (DEP) spots in waxy and non-waxy wheats, which belonged mainly to starch synthase (SS) I, SS IIa and granule-bound SS I. Most DEPs involved in amylopectin synthesis showed a similar expression pattern during grain development, suggesting relatively independent amylose and amylopectin synthesis pathways. Phosphoproteome analysis of starch granule-binding proteins, using TiO2 microcolumns and LC-MS/MS, showed that the total number of phosphoproteins and their phosphorylation levels in ND5181 were significantly higher than in SN119, but proteins controlling amylopectin synthesis had similar phosphorylation levels. Our results revealed the lack of amylose did not affect the expression and phosphorylation of the starch granule-binding proteins involved in amylopectin biosynthesis.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins

Chen

Zhou

Liu

et al. 2016

Sci Rep

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“…As reported in our earlier study (Hu et al, 2010), total proteins from the eighth leaf of the maize plants were extracted according to the method reported by Wang et al (2013) and Zhang et al (2014). Briefly, ~0.5 g fresh leaves from each biological replicate were ground into a fine power in liquid nitrogen using a mortar and pestle and further ground in 4 ml of SDS buffer (30% sucrose, 2% SDS, 100 mM Tris-HCl, pH 8.0, 50 mM EDTA-Na 2 , 20 mM DTT) and 4 ml phenol (Tris-buffered, pH 8.0), then 1 mM phenylmethanesulfonyl fluoride (PMSF) and PhosSTOP phosphatase inhibitor cocktail (one tablet/10 ml; Roche, Basel, Switzerland) was added to inhibit protease and phosphatase activity.…”

Section: Methodsmentioning

confidence: 99%

The Difference of Physiological and Proteomic Changes in Maize Leaves Adaptation to Drought, Heat, and Combined Both Stresses

et al. 2016

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At the eight-leaf stage, maize is highly sensitive to stresses such as drought, heat, and their combination, which greatly affect its yield. At present, few studies have analyzed maize response to combined drought and heat stress at the eight-leaf stage. In this study, we measured certain physical parameters of maize at the eight-leaf stage when it was exposed to drought, heat, and their combination. The results showed an increase in the content of H2O2 and malondialdehyde (MDA), and in the enzyme activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR), but a decrease in the quantum efficiency of photosystem II (ΦPSII). The most obvious increase or decrease in physical parameters was found under the combined stress condition. Moreover, to identify proteins differentially regulated by the three stress conditions at the eight-leaf stage, total proteins from the maize leaves were identified and quantified using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. In summary, the expression levels of 135, 65, and 201 proteins were significantly changed under the heat, drought and combined stress conditions, respectively. Of the 135, 65, and 201 differentially expressed proteins, 61, 28, and 16 responded exclusively to drought stress, heat stress, and combined stress, respectively. Bioinformatics analysis implied that chaperone proteins and proteases play important roles in the adaptive response of maize to heat stress and combined stress, and that the leaf senescence promoted by ethylene-responsive protein and ripening-related protein may play active roles in maize tolerance to combined drought and heat stress. The signaling pathways related to differentially expressed proteins were obviously different under all three stress conditions. Thus, the functional characterization of these differentially expressed proteins will be helpful for discovering new targets to enhance maize tolerance to stress.

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“…PP2C is known to be a negative regulator for plant drought tolerance in the abscisic acid (ABA) signaling pathway, which can inhibit the activity of SnRK, leading to a decrease of the phosphorylation of its substrates in the signaling cascade [83,84,85]. Both phosphorylation level [40,44] and abundance of PP2C [41,86] were obviously affected by drought stress. These data suggest that the PP2C-involved ABA signaling pathway is crucial for drought response.…”

Section: Drought Sensing and Signalingmentioning

confidence: 99%

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

Wang

Cai

et al. 2016

IJMS

203

143

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Plant drought tolerance is a complex trait that requires a global view to understand its underlying mechanism. The proteomic aspects of plant drought response have been extensively investigated in model plants, crops and wood plants. In this review, we summarize recent proteomic studies on drought response in leaves to reveal the common and specialized drought-responsive mechanisms in different plants. Although drought-responsive proteins exhibit various patterns depending on plant species, genotypes and stress intensity, proteomic analyses show that dominant changes occurred in sensing and signal transduction, reactive oxygen species scavenging, osmotic regulation, gene expression, protein synthesis/turnover, cell structure modulation, as well as carbohydrate and energy metabolism. In combination with physiological and molecular results, proteomic studies in leaves have helped to discover some potential proteins and/or metabolic pathways for drought tolerance. These findings provide new clues for understanding the molecular basis of plant drought tolerance.

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Phosphoproteome analysis reveals new drought response and defense mechanisms of seedling leaves in bread wheat (Triticum aestivum L.)

Cited by 136 publications

References 88 publications

Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins

Biosynthesis and Regulation of Wheat Amylose and Amylopectin from Proteomic and Phosphoproteomic Characterization of Granule-binding Proteins

The Difference of Physiological and Proteomic Changes in Maize Leaves Adaptation to Drought, Heat, and Combined Both Stresses

Drought-Responsive Mechanisms in Plant Leaves Revealed by Proteomics

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