Phosphonic Acid Fluorescent Organic Nanoparticles for High-Contrast and Selective Staining of Gram-Positive Bacteria

Abstract

Fast and selective detection of pathogens represents high challenges given the considerably high proliferation rate and mutation potential of bacteria against antibiotics. With this aim, anionic red-orangeemitting fluorescent organic nanoparticles (FONs), characterized by high brightness and photostability, are developed to selectively stain Staphylococcus aureus after only 5 min of exposure. No cytotoxicity effects are observed as a result of the negatively charge surface of the employed FONs. By contrast, no staining can be observed with Pseudomonas aeruginosa strains. The exclusive labeling of Gram-positive bacteria is ascribed to originate from the phosphonic acid moieties incorporated in the FON-constituting fluorophores because model FONs, devoid of phosphonic acids, show no adhesion under the same experimental conditions. Tight hydrogen bonding between the FON acidic units and the peptidoglycan (PG) layers comprising the outer wall of S. aureus is suspected to be the prevailing factor for the encountered selective interactions. PG layers from S. aureus are employed to apprehend the interactions developed between FONs and the bacteria membrane. Correlative light electron microscopy using confocal fluorescence microscopy and SEM reveal FONs mainly located at extensively reorganized or "dented" PG areas. Such privileged localizations tend to suggest multivalent physicochemical interactions to aggregate a multifold of nanoparticles. Finally, spectral follow-up of the FON-stained bacteria membrane shows significant hypsochromic shift of the fluorescence emission, signaling progressive disassembly of FONs and a change of the surrounding polarity. This feature offers promising perspectives to use doped FONs as theranostic agents to liberate encapsulated antibiotics upon FON disintegration inside the bacteria membrane.

Tuning of the Coordination and Emission Properties of 4-Amino-2,1,3-Benzothiadiazole by Introduction of Diphenylphosphine Group

“…Phosphazanes exhibiting luminescent properties can be obtained by introducing into the molecular structure a chromophore, such as, for example, a btd unit. However, known phosphorus-containing benzothiadiazoles are relatively scarce: they are mainly limited to derivatives with phosphate groups bonded to the btd moiety via various linkers; − the compounds with the P­(III)–N unit directly bonded to btd have not been practically described in the literature yet. The coordination chemistry of these btd derivatives is of particular interest, as it allows expansion of the range of their functional properties .…”

Structural and Photophysical Properties of 2,1,3-Benzothiadiazole-Based Phosph(III)azane and Its Complexes

“…A number of btd derivatives bearing N-donor functional groups were recently studied [2,[10][11][12][13]. Phosphorus-containing btds are much less abundant; they are mainly limited to derivatives with phosphate groups [14][15][16][17][18].…”

Non-leaching, Highly Biocompatible Nanocellulose Surfaces That Efficiently Resist Fouling by Bacteria in an Artificial Dermis Model

“…The tight electrostatic interactions that positively charged nanomaterials establish upon contact with the negatively charged functional groups at the surface of bacteria have been extensively exploited in the design of contact-active antibacterial surfaces, albeit the well-documented toxicity of polycationic compounds. − In contrast, anionic surfaces circumvent this toxicity issue because they fail to induce the strong membrane polarization that leads to cell lysis. Nonetheless, they remain greatly unexplored.…”