Phospholipids in lipoproteins: compositional differences across VLDL, LDL, and HDL in pregnant women

Abstract

ObjectiveThe aim of this study was to analyse the differences in the phospholipid composition of very low density (VLDL), low density (LDL) and high density lipoprotein (HDL) monolayers in pregnant lean and obese women.MethodsLDL, HDL, and VLDL were isolated from plasma samples of 10 lean and 10 obese pregnant women, and their species composition of phosphatidylcholines (PC) and sphingomyelins (SM) was analysed by liquid-chromatography tandem mass-spectrometry. Wilcoxon-Mann-Whitney U test and principal component analysis (PCA) were used to investigate if metabolite profiles differed between the lean/obese group and between lipoprotein species.ResultsNo significant differences have been found in the metabolite levels between obese and non-obese pregnant women. The PCA components 1 and 2 separated between LDL, HDL, and VLDL but not between normal weight and obese women. Twelve SM and one PCae were more abundant in LDL than in VLDL. In contrast, four acyl-alkyl-PC and two diacyl-PC were significantly higher in HDL compared to LDL. VLDL and HDL differed in three SM, seven acyl-alkyl-PC and one diacyl-PC (higher values in HDL) and 13 SM (higher in VLDL). We also found associations of some phospholipid species with HDL and LDL cholesterol.ConclusionIn pregnant women phospholipid composition differs significantly in HDL, LDL and VLDL, similar to previous findings in men and non-pregnant women. Obese and lean pregnant women showed no significant differences in their lipoprotein associated metabolite profile.Electronic supplementary materialThe online version of this article (10.1186/s12944-019-0957-z) contains supplementary material, which is available to authorized users.

Co-transplantation of Human Fetal Mesenchymal and Hematopoietic Stem Cells in Type 1 Diabetic Mice Model

“…Lag phase was happened immediately after re-expansion. In this study, the lag period lasted 2–3 days similar to other reports (25, 35). Lag phase allows the cells to recover from detachment and harvesting, repair the cytoskeleton, and restart the cell cycle.…”

A high-throughput platform for detailed lipidomic analysis of a range of mouse and human tissues

“…For example, comparing the ratio of particular lipids to one another can be used to infer the activity of particular enzymes involved in fatty acid biosynthesis and modification. A variety of lipid abundance ratios have been used for this, either using the ratio of particular fatty acids or lipids that comprise them: fatty acid elongase (FA(18:0)/ FA(16:0); PC(36:4)/PC(34:2)) [45][46][47], Stearoyl co-acetyl desaturase (CE(16:1)/CE(16:0); CE(18:3)/CE(18:2); PC(32:1)/PC/32:0)) [48,49], fatty acid desaturase 1 (FADS1, PC(38:4)/PC (38:3); TG(54:4)/TG(54:3)) [45,46], and fatty acid desaturase 2 (FADS2, PC(36:3)/PC(36:2); TG(50:3)/TG(50:2)) [45,46].…”

Hypertriglyceridemia triggered acute pancreatitis in pregnancy – diagnostic approach, management and follow-up care

“…Normal pregnancy is characterized by adaptive lipid metabolism changes meant to ensure placental needs and glucose and lipid requirements of the growing fetus: increased glucose production, progesterone synthesis and lipogenesis, diminished lipolysis [13,14]. In women with abnormal lipoprotein metabolism these changes lead to severe HTG and may precipitate pancreatitis [15].…”