Phospholipids from the chemoautotroph Ferrobacillus ferrooxidans

Korczyński, Mariusz; Agate, A. D.; Lundgren, D. G.

doi:10.1016/0006-291x(67)90505-0

Cited by 15 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mechanism of iron oxidation is proposed [24] in which the complexing of ferrous ion with molecular oxygen precedes the electron transport involving sulfate [100][101][102] and phosphatidylserine [73,103] in the oxidation. The isolation of cytochrome c and cytochrome a from T. ferrooxidans [ 104] allowed the postulation of iron oxidation through the cytochrome system with oxygen as the final electron acceptor [96,[104][105][106]:…”

Section: ) Oxidation Of Ferrous Ionmentioning

confidence: 99%

See 1 more Smart Citation

The role of Thiobacillus ferrooxidans in hydrometallurgical processes

Torma

1977

Advances in Biochemical Engineering/Biotechnology

105

View full text Add to dashboard Cite

Section: ) Oxidation Of Ferrous Ionmentioning

confidence: 99%

“…coli strains [70][71][72]. Phospholipids and neutral lipids are also related to the cell envelope structure [73]. The cytoplasma of T. ferrooxidans contains ribosomes, nuclear materials and cell inclusions [52,68,69].…”

Section: C) Chemical Composition and Structure Of T Ferrooxidansmentioning

confidence: 99%

The role of Thiobacillus ferrooxidans in hydrometallurgical processes

Torma

1977

Advances in Biochemical Engineering/Biotechnology

105

View full text Add to dashboard Cite

“…Free granule-like material, ranging in sizes from 50 to 100 m,u, is visible in the background. The amount of material removed is about 25% of the dry weight of the cell and has been studied separately (6). More surface alteration is noted after treatments involving urea, 2-mercaptoethanol, and iodoacetic acid extraction and pepsin digestion (Fig.…”

Section: Resultsmentioning

confidence: 99%

Peptidoglycan of a Chemolithotrophic Bacterium, Ferrobacillus ferrooxidans

Wang

Lundgren

1968

J Bacteriol

View full text Add to dashboard Cite

The rigid layer (peptidoglycan) of the wall of the chemolithotroph Ferrobacillus ferrooxidans was isolated after various chemical treatments. The removal of specific components was followed by noting in an electron microscope changes in the appearance of the cell surface. The final peptidoglycan was virtually free from proteins and was sensitive to the action of lysozyme. Results of chemical analyses of acidhydrolyzed peptidoglycan revealed three major amino acids and two amino sugars: glutamic acid, a-diaminopimelic acid, alanine, glucosamine, and muramic acid in a ratio of 1:1:2.33:062:088.

show abstract

“…Much of the recent study with this organism has focused on the cell envelope layers of the organism (5,8,15,29,37,39,40). The objective in many of these studies has been to discover information about the structure or organization of the cell surface which would help explain the acidophilic nature of the cells or their ability to utilize such diverse energy sources.…”

mentioning

confidence: 99%

“…Phosphatidyl serine has been found to bind iron efficiently (2) and may function to bind iron to the cell surface in nature. Unfortunately, reports have been contradictory as to the presence of this phospholipid in iron-grown cells (15,33). The peptidoglycan and lipopolysaccharide (LPS) layers of iron-grown cells have been partially characterized (40).…”

mentioning

confidence: 99%

Physical and chemical studies of Thiobacillus ferroxidans lipopolysaccharides

Hirt¹,

Vestal²

1975

J Bacteriol

View full text Add to dashboard Cite

The lipopolysaccharides (LPS) of the obligate acidophile Thiobacillus ferrooxidans grown on iron, sulfur, and glucose as energy sources were examined for various physical and chemical properties. Both qualitative and quantitative variations were found among the three preparations. The LPS extracted from iron-grown cells (Fe-LPS) contained less than 3% protein compared to 18 to 25%c in LPS extracted from either sulfur-grown cells (S-LPS) or glucose-grown cells (G-LPS). S-LPS showed two distinct sedimentable species. 61S and 9.3S, which could be fractionated on a column of Sepharose 4B. The relative densities of' both S-LPS and G-LPS were found to be significantly greater than that of Fe-LPS. Spectral differences were noted when each LPS was reacted with a carbocyanine dye. Fe-LPS showed a single absorbance maximum at 472 nm, S-LPS displayed its maximum at 650 nm, and G-LPS showed two maxima, the first at 468 nm and the other at 655 nm. Analysis of the methyl ester derivatives of the LPS t'atty acids using gas chromatography-mass spectrometry revealed the presence of' a very stable species, tentatively identified as a methoxy methyl ester with a formula of CH3-O-C10H10-COOCH3, as the major component from each LPS. 3-Hydroxymyristic acid was found only in Fe-LPS.

show abstract

Phospholipids from the chemoautotroph Ferrobacillus ferrooxidans

Cited by 15 publications

References 14 publications

The role of Thiobacillus ferrooxidans in hydrometallurgical processes

The role of Thiobacillus ferrooxidans in hydrometallurgical processes

Peptidoglycan of a Chemolithotrophic Bacterium, Ferrobacillus ferrooxidans

Physical and chemical studies of Thiobacillus ferroxidans lipopolysaccharides

Contact Info

Product

Resources

About