Peptidoglycan of a Chemolithotrophic Bacterium,
            <i>Ferrobacillus ferrooxidans</i>

Wang, W. S.; Lundgren, D. G.

doi:10.1128/jb.95.5.1851-1856.1968

Cited by 11 publications

(4 citation statements)

References 16 publications

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“…Figure 6 is a carbon replica electron micrograph of control cells treated for 25 min. The cell surface exhibits the typical rugose appearance described by Wang and Lundgren (36).…”

Section: Cmentioning

confidence: 67%

Leakage of cellular material from Thiobacillus ferrooxidans in the presence of organic acids

Tuttle¹,

Dugan²

1977

Appl Environ Microbiol

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Thiobacillus ferrooxidans cells released varying amounts of iron, phosphate, sugar, ribonucleic acid, deoxyribonucleic acid, and substances that absorb light at both 260 and 280 nm when exposed to 10-2 to 10-1 M concentrations of these organic acids: propionic, butyric, valeric, hexanoic, and oxalacetic. These acids also retarded iron oxidation by the cells. Electron microscopic observation of cells after exposure to the organic acids showed varying degrees of cell envelope disruption, suggesting that the mode of inhibition of autotrophic iron oxidation in the cell involves interference with the function of the cell envelope, possibly the cell membrane.

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“…Figure 6 is a carbon replica electron micrograph of control cells treated for 25 min. The cell surface exhibits the typical rugose appearance described by Wang and Lundgren (36).…”

Section: Cmentioning

confidence: 67%

Leakage of cellular material from Thiobacillus ferrooxidans in the presence of organic acids

Tuttle¹,

Dugan²

1977

Appl Environ Microbiol

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“…Gram-negative bacteria typically possess peptidoglycan in which the glycan chain consists of alternating units of Glc and Mur and the peptide linked to the Mur is a four-unit chain of Ala-Glu-Dpm-Ala. Hydrolysates of such peptidoglycans contain Ala, Glu, Dpm, Mur and Glc in molar ratios of 2:1:1:1:1 (43). Exceptions have been reported in which the Mur and Glc are not present in equimolar amounts, but neither amino sugar was found to exceed the proportion of Dpm (22,56).…”

Section: Discussionmentioning

confidence: 97%

Novel peptidoglycans in Caulobacter and Asticcacaulis spp

Poindexter¹,

Hagenzieker²

1982

J Bacteriol

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Peptidoglycan sacculi free of poly-beta-hydroxybutyric acid were prepared from whole cells of four species of Caulobacter and two species of Asticcacaluis and from morphological mutants of Caulobacter crescentus and Caulobacter leidyi. Acid hydrolysates of the sacculi were analyzed quantitatively, and each of the hydrolysates was found to contain significant amounts of only five ninhydrin-reactive compounds: alanine, glutamic acid, alpha , omega-diaminopimelic acid, muramic acid, and glucosamine. Four types of peptidoglycans were distinguishable on the basis of the molar ratios among these five compounds. The respective ratios were as follows: in C. leidyi, 2:1:1:1:0.8; in Asticcacaulis biprosthecum, 1.7:1.6:1.1:0.7; in the cells of the remaining species, 2:1:1:1.2:0.8; and in stalks shed by the abscission mutant 2NY66, 2:1:1:1:1.67. Thus, in addition to some species differences among these caulobacters, it was found that the peptidoglycan sacculus of the stalked C. crescentus cell is chemically differentiated; the cellular peptidoglycan is richer in muramic acid than is the peptidoglycan of typical gram-negative bacteria, and the peptidoglycan of the stalk is correspondingly rich in glucosamine. Empirical formulas for the repeating units of the peptidoglycans have been inferred on the basis of the molar ratios of their amino components.

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“…However, development of an appropriate methodology for muropeptide analysis was not possible until the advent of advanced chromatographic techniques in the early 1980s. Prior to this, most information about wall components was gleaned from amino acid analysis (Sutow and Welker, 1967;Boylen and Ensign, 1968;Wang and Lundgren, 1968) or paper chromatography (Kandler et al, 1968;de la Rosa et al, 1985) of lysozyme-digested cell walls. These tedious, low-resolution techniques allowed for the detection of at most six different muropeptide species.…”

Section: Historical Overview Of Applications Of Chromatography To Pg mentioning

confidence: 99%

Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly

Desmarais

Pedro

Cava

et al. 2013

Molecular Microbiology

105

112

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The peptidoglycan (PG) cell wall is a unique macromolecule responsible for both shape determination and cellular integrity under osmotic stress in virtually all bacteria. A quantitative understanding of the relationships between PG architecture, morphogenesis, immune system activation, and pathogenesis can provide molecular-scale insights into the function of proteins involved in cell-wall synthesis and cell growth. High Performance Liquid Chromatography (HPLC) has played an important role in our understanding of the structural and chemical complexity of the cell wall by providing an analytical method to quantify differences in chemical composition. Here, we present a primer on the basic chemical features of wall structure that can be revealed through HPLC, along with a description of the applications of HPLC PG analyses for interpreting the effects of genetic and chemical perturbations to a variety of bacterial species in different environments. We describe the physical consequences of different PG compositions on cell shape, and review complementary experimental and computational methodologies for PG analysis. Finally, we present a partial list of future targets of development for HPLC and related techniques.

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Peptidoglycan of a Chemolithotrophic Bacterium, Ferrobacillus ferrooxidans

Cited by 11 publications

References 16 publications

Leakage of cellular material from Thiobacillus ferrooxidans in the presence of organic acids

Leakage of cellular material from Thiobacillus ferrooxidans in the presence of organic acids

Novel peptidoglycans in Caulobacter and Asticcacaulis spp

Peptidoglycan at its peaks: how chromatographic analyses can reveal bacterial cell wall structure and assembly

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