PHOSPHOLIPIDS FROM PUMPKIN (<i>Cucurbita moschata</i> (Duch.) Poir) SEED KERNEL OIL AND THEIR FATTY ACID COMPOSITION

Abstract

The phospholipids (PL) of pumpkin (Cucurbita moschata (Duch) Poir) seed kernel and their fatty acid composition were investigated. The crude oil was obtained by maceration with isopropanol followed by steps of extraction yielded polar lipids. The quantitative determination of PLs content of the dried pumpkin seed kernel and their polar lipids were calculated based on the elemental phosphorus (P) contents which was determined by means of spectrophotometric methods. PL classes were separated from polar lipids via column chromatography. The fatty acid composition of individual PL was identified by gas chromatography-mass spectrometry (GC-MS). The total of PL in the pumpkin seed kernels was 1.27% which consisted of phosphatidylcholine (PC), phosphatidylserine (PS) and phosphatidyletanolamine (PE). The predominant fatty acids of PL were oleic and palmitic acid in PC and PE while PS's fatty acid were dominantly consisted of oleic acid and linoleic acid.

Seed oil content and fatty acid composition in a genebank collection of Cucurbita moschata Duchesne and C. argyrosperma C. Huber

“…Al-Khalifa (1996) noted that linoleic acid was the predominant fatty acid in C. moschata (53.2%) followed by oleic (26.2%), palmitic (13.1%) and stearic (6.0%). Raharjo et al (2011) reported that the predominant fatty acids associated with seed phospholipids were oleic and palmitic in phosphatidylcholine and phosphatidylethanolamine, and oleic and linoleic in phosphatidylserine. Fokou et al (2009) reported an average fatty acid composition (four varieties) of C. moschata as 49.5% linoleic, 19.6% oleic, 19.2% palmitic and 9.2% stearic with traces (1.7%) of arachidonic (C20:0) acid.…”

“…In more recent reports, Applequist et al (2006) reported average fatty content values (four varieties) in C. moschata of 55.0% linoleic, 19.2% oleic, 15.8% palmitic and 6.9% stearic. Raharjo et al (2011) reported that the predominant fatty acids associated with seed phospholipids were oleic and palmitic in phosphatidylcholine and phosphatidylethanolamine, and oleic and linoleic in phosphatidylserine. Kim et al (2012) identified four fatty acids in the seed of C. moschata , these being linoleic (35.7%), oleic (31.3%), palmitic (12.8%) and stearic (7.3%).…”

Phytochemical Analysis of Bioactive Extracts and Seed Oil of Three Euphorbia Species from Algerian Flora by LC-MS and GC-MS

“…The fatty acid composition is determined as the methyl esters of fatty acids by gas-liquid chromatography. Methyl esters of the fatty acids (FAME) of the three seed oils were prepared according to a convenient method [20], using BF3-methanol at 60 °C.…”