Phospholipids and cholesterol in brush border and basolateral membranes from rat intestinal mucosa

Chapelle, S.; Gilles-Baillien, M.

doi:10.1016/0005-2760(83)90017-6

Cited by 42 publications

(14 citation statements)

References 17 publications

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“…However, Chapelle and Gilles-Baillien [12] have recently reported that other epithelial cells share this asymmetric distribution of membrane lipid components. They reported that BBM from intestinal mucosa has an increased content of sphingomyelin and an increased cholesterol-tophospholipid ratio when compared to BLM from the same tissue.…”

Section: Discussionmentioning

confidence: 97%

Renal cortical brush-border and basolateral membranes: Cholesterol and phospholipid Composition and relative turnover

Molitoris¹,

Simon²

1985

J. Membrain Biol.

125

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A new procedure for the rapid isolation of renal cortical brush-border and basolateral membranes from the same homogenate is described. Brush-border membranes isolated using Mg2+-EGTA precipitation were enriched 18-fold for leucine aminopeptidase and had a recovery of 32.5%. Basolateral membrane fractions were isolated using a discontinuous sucrose gradient and showed an enrichment of 10.7-fold and recovery of 12.8% using (Na+,K+)-ATPase as a marker enzyme. Lipid analysis using two-dimensional TLC separation of phospholipids and gas liquid chromatography for cholesterol showed marked differences in the lipid composition of the brush-border and basolateral membranes. The brush-border membrane had increased sphingomyelin, phosphatidylserine, ethanolamine plasmalogens, and an increased cholesterol-to-phospholipid and sphingomyelin-to-phosphatidylcholine ratio compared to the basolateral membrane. The relative turnover of total membrane and individual phospholipid species using a double isotope ratio method was carried out. Phospholipids were labeled with either phosphorus 32 and 33 or acetate (3H, 1-14C). The relative turnover of phospholipid species and cholesterol differed strikingly. Phosphatidylcholine showed a high turnover, phosphatidylethanolamine and phosphatidylinositol had intermediate values and sphingomyelin, phosphatidylserine and cholesterol had low relative turnover rates. The order of phospholipid class relative turnover was independent of the labeled precursor used. The brush-border membrane had a significantly reduced relative turnover rate for total membrane phospholipids, sphingomyelin and cholesterol compared to the basolateral membrane. These data show marked differences in the lipid composition and relative turnover rates of the phospholipid species of the brush-border and basolateral membranes.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 97%

Renal cortical brush-border and basolateral membranes: Cholesterol and phospholipid Composition and relative turnover

Molitoris¹,

Simon²

1985

J. Membrain Biol.

125

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“…Recent evidence [4,8,15,17,23] clearly indicates apical and basolateral membranes of epithelial cells differ widely in lipid composition. How epithelial cells achieve and maintain this polarity is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…Large differences in the phospholipid composition of apical and basolateral (BLM) membrane fractions of epithelial cells such as renal proximal tubule [8,17], small intestine [4], hepatocytes [15], and MDCK cells [23] have recently been described. The differences in renal proximal tubule cells are quite pronounced with the apical or brush border (BBM) having a sphingomyelin to phosphatidylcholine ratio of 2.1 while the BLM ratio is only 0.4 [17].…”

Section: Introductionmentioning

confidence: 99%

Maintenance of epithelial surface membrane lipid polarity: A role for differing phospholipid translocation rates

Molitoris

Simon

1986

J. Membrain Biol.

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Large differences in lipid composition of apical and basolateral membranes from epithelial cells exist. To determine the responsible mechanism(s), rat renal cortical brush border and basolateral membrane phospholipids were labeled using 32P and either [3H]-glycerol or [2-3H] acetate for incorporation and degradation studies, respectively. Brush border and basolateral membrane fractions were isolated simultaneously from the same cortical homogenate. Different phospholipid classes were degraded at variable rates with phosphatidylcholine having the fastest decay rate. Decay rates for individual phospholipid classes were, however, similar in both brush border and basolateral membrane fractions. In phospholipid incorporation studies, again, large variations existed between individual phospholipid classes with phosphatidylcholine and phosphatidylinositol showing the most rapid rates of incorporation. Sphingomyelin and phosphatidylserine showed extremely slow incorporation rates and did not enter into the isotopic decay phase for 48 hr. In contrast to degradation studies, however, the same phospholipid class labeled the two surface membrane domains at highly variable rates. The difference in these rates, with the exception of phosphatidylinositol, were identical to the differences in phospholipid compositions between the two membranes. For example, phosphatidylcholine was incorporated into the basolateral membrane 2.5 X faster than into the brush border membrane and its relative composition was 2.5 X greater in the basolateral membrane. The opposite was true for sphingomyelin. These results indicate incorporation and not degradation rates of individual phospholipids play a major role in regulating the differing phospholipid composition of brush border and basolateral membranes.

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“…For example, in the rat intestinal mucosa, cholesterol is found in higher concentrations in the basolateral membranes than in the brush border membranes. 8 Filipin binding (to cholesterol) studies have suggested that cholesterol is inhomogeneously distributed within the Golgi stacks, with greater cholesterol content found in the cisternae of the Golgi located near the plasma membrane (trans Golgi) than in the cisternae of the Golgi located near the endoplasmic reticulum (cis Golgi). 9 As seen in chapter "Membrane Fusion," all these membranes are connected through vesicular traffic and thus it makes some sense that the portions of the Golgi closest to the endoplasmic reticulum would have a cholesterol level closer to the cholesterol content of the latter membrane, for example.…”

Section: Distribution Of Cholesterol In Cellsmentioning

confidence: 99%

Cholesterol and Related Sterols

Yèagle

2016

The Membranes of Cells

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Phospholipids and cholesterol in brush border and basolateral membranes from rat intestinal mucosa

Cited by 42 publications

References 17 publications

Renal cortical brush-border and basolateral membranes: Cholesterol and phospholipid Composition and relative turnover

Renal cortical brush-border and basolateral membranes: Cholesterol and phospholipid Composition and relative turnover

Maintenance of epithelial surface membrane lipid polarity: A role for differing phospholipid translocation rates

Cholesterol and Related Sterols

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