Phospholipidic signaling and vanillin production in response to salicylic acid and methyl jasmonate in Capsicum chinense J. cells

Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota

“…In addition, when C. chinense SCC was elicited with 200 μM salicylic acid, vanillin levels (around 67 µg g DW -1 ) were higher than those found in control cells (around 25 µg g DW -1 ) [59]. Similarly, Altúzar-Molina et al [60] showed that low doses of methyl jasmonate (25 µM) also induced the production of vanillin (around 40 µg g DW -1 ) in C. chinense SCC. Also, callus cultures from leaves of Hemidesmus indicus R. also were able to produce vanillin in a culture medium without hormone, although the levels reached were very low (1,02 µg g DW -1 ) [61].…”

Phospholipase signaling is modified differentially by phytoregulators inCapsicum chinenseJ. cells

“…the phospholipidic signaling pathway forms part of the plant response to several phytoregulators, such as salicylic acid (Sa) and methyl jasmonate (mJ), which have been widely used to stimulate secondary metabolite production in cell cultures. 1 Furthermore, it has been reported that the levels of such phytoregulators as Sa and mJ can increase in response to stressful conditions. 2,3 the phospholipidic signal transduction system involves the generation of second messengers by the hydrolysis of phospholipids.…”

“…23 SA and MJ mediate cell responses to stress, and these compounds have been used as elicitors to study secondary metabolite production in suspension cell cultures of different plants, including Capsicum. 1,24,25 The main goal of this work was to investigate the effects of SA and MJ on the activities of two phospholipases (PLC and PLD) in Capsicum chinense cells on different days of the culture cycle to complement data reported in. 1 We used an enzymatic in vitro assay with a radiolabeled substrate in both assays, 3 [H]-PtdIns(4,5)P 2 and 3 [H]-PC, measuring the formation of Ins(1,4,5)P 3 for the PLC activity and choline for the PLD activity, modulate the PLC and PLD activities through different phospholipid signal transduction pathways (Fig.…”

“…The experimental procedure was performed as previously reported. 1 The results obtained (Fig. 1) show that the PLC activity in the cells on day 7 of the culture was stimulated at 25 μM and 100 μM MJ concentrations (Fig.…”

The regulatory mechanism of fungal elicitor-induced secondary metabolite biosynthesis in medical plants

“…Indeed, both GTP-binding activity and GTPase activity of the plasma membrane are stimulated by elicitor, and suramin and cholera toxin affected G-protein activities, which suggest that Ca 2+ and G-proteins may mediate elicitor signals to the jasmonate pathway, and the jasmonate signaling pathway may then lead to the production of beta-thujaplicin (Zhao et al 2003).The phosphoinositide degradation is found in a variety of plants. PLC is activated by Ca 2+ and IP3 can transportCa 2+ from intracellular stores into the cytoplasm (Aharon et al 1998).Thus, IP3-Ca 2+ signaling pathway is thought to be the main regulatory mechanism of inducing phytoalexin (Altuzar-Molina et al 2011)and cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) are involved in anion channel and Ca 2 + flow, both are important signal channels of accumulation of secondary metabolites in many medical plants (Pietrowska-Borek et al 2014, Wu et al 2008. Fungal elicitor is recognized by the receptorsin the plant, followed with Ca 2+ burst in many medical plants (Du et al 2015, Genre et al 2013, Lecourieux et al 2002.Ca 2+ is a significant second messager involved in the signal transduction progress of endophytic fungi-induced secondary metabolite accumulation in medical plants (Vasyukova et al 2001 to the enhancement of the activity of phospholipase C, which activates the biosynthetic pathways of polyphosphoinositide sugar leading in the improvement of the quantity of phytoalexin.…”