Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota

Miras-Moreno, Begoña; Almagro, Lorena; Pedreño, María A.; Sabater-Jara, Ana Belén

doi:10.1016/j.plantsci.2016.06.008

Cited by 23 publications

(13 citation statements)

References 55 publications

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“…Durante et al [16] reported a negligible effect of CDs on cell growth when employing PSCC from Artemisia annua treated with 50 mM M–β–CD. In contrast, a reduction on PSCC growth from Daucus carota was reported when they were treated with 50 mM M–β–CD [17], as well as cell cultures from Vitis vinifera, under the same conditions [18].…”

Section: Resultsmentioning

confidence: 99%

“…Enhancement of extracellular levels of phenols in PSCC elicited with CDs has already been reported in the literature. For example, in cells elicited with M–β–CD (50 mM), vanillin concentration was boosted at the extracellular level in PSCC from D. carota and trans-resveratrol was exogenously accumulated in PSCC from V. vinifera [17,19]. The reasons for such an increase are generally attributed to the presence of elicitors, which trigger the stress response in the cell with a concomitant enhancement on the intracellular production of secondary metabolites.…”

Section: Resultsmentioning

confidence: 99%

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Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

García-Pérez

Losada‐Barreiro

Gallego

2019

IJMS

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The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC) with cyclodextrins (CDs) as an alternative method for the production of bioactive compounds from Bryophyllum species. For this purpose, we analyzed the effects of methyl–β–cyclodextrin and 2–hydroxypropyl–β–cyclodextrin on cell culture growth and on the intra- and extracellular production of phenols and flavonoids. Results clearly show that CDs enhance the biosynthesis of polyphenols by PSCC favoring their accumulation outside the cells. CDs shift the homeostatic equilibrium by complexing extracellular phenolics, causing stress in cells that respond by increasing the production of intracellular phenolics. We also analyzed the radical scavenging activity of the culture medium extracts against 2,2–diphenyl–1–pycrilhydrazyl (DPPH) radical, which increased with respect to the control samples (no added CDs). Our results suggest that both the increase in the production of polyphenols and their radical scavenging activity are a consequence of their inclusion in the CD cavities. Overall, based on our findings, CDs can be employed as hosts for increasing the production of polyphenols from Bryophyllum species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

García-Pérez

Losada‐Barreiro

Gallego

2019

IJMS

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“…Apart from the positive effect of HP-␤-CD on steroid conversion, the enhanced catalytic activity of KsdD in the presence of HP-␤-CD increases the ADD-to-AD ratio. In plant cells, CDs are used as elicitors to improve the production of artemisinin, trans-resveratrol, indole alkaloid, phytosterols, and phenolic compounds from cell suspension cultures of plants (43)(44)(45)(46). However, studies have rarely reported the effect of CDs on steroid metabolic pathways of M. neoaurum.…”

Section: Discussionmentioning

confidence: 99%

The Sterol Carrier Hydroxypropyl-β-Cyclodextrin Enhances the Metabolism of Phytosterols by Mycobacterium neoaurum

Shen

et al. 2020

Appl Environ Microbiol

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Androst-4-ene-3,17-dione (AD) and androst-1,4-diene-3,17-dione (ADD) are valuable steroid pharmaceutical intermediates obtained by soybean phytosterol biotransformation by Mycobacterium. Cyclodextrins (CDs) are generally believed to be carriers for phytosterol delivery and can improve the production of AD and ADD due to their effects on steroid solubilization and alteration in cell wall permeability for steroids. To better understand the mechanisms of CD promotion, we performed proteomic quantification of the effects of hydroxypropyl-β-CD (HP-β-CD) on phytosterol metabolism in Mycobacterium neoaurum TCCC 11978 C2. Perturbations are observed in steroid catabolism and glucose metabolism by adding HP-β-CD in a phytosterol bioconversion system. AD and ADD, as metabolic products of phytosterol, are toxic to cells, with inhibited cell growth and biocatalytic activity. Treatment of mycobacteria with HP-β-CD relieves the inhibitory effect of AD(D) on the electron transfer chain and cell growth. These results demonstrate the positive relationship between HP-β-CD and phytosterol metabolism and give insight into the complex functions of CDs as mediators of the regulation of sterol metabolism. IMPORTANCE Phytosterols from soybean are low-cost by-products of soybean oil production and, owing to their good bioavailability in mycobacteria, are preferred as the substrates for steroid drug production via biotransformation by Mycobacterium. However, the low level of production of steroid hormone drugs due to the low aqueous solubility (below 0.1 mmol/liter) of phytosterols limits the commercial use of sterol-transformed strains. To improve the bioconversion of steroids, cyclodextrins (CDs) are generally used as an effective carrier for the delivery of hydrophobic steroids to the bacterium. CDs improve the biotransformation of steroids due to their effects on steroid solubilization and alterations in cell wall permeability for steroids. However, studies have rarely reported the effects of CDs on cell metabolic pathways related to sterols. In this study, the effects of hydroxypropyl-β-CD (HP-β-CD) on the expression of enzymes related to steroid catabolic pathways in Mycobacterium neoaurum were systematically investigated. These findings will improve our understanding of the complex functions of CDs in the regulation of sterol metabolism and guide the application of CDs to sterol production.

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“…Bioactive compound extractions from dried broccoli by-products were performed using different sources of plant material, i.e., leaves, stems and separately or in combination. In order to compare the bioactive compounds present in the SFE extracts with those obtained from conventional extracts, the CE of carotenoids, chlorophylls, total phenolic compounds and phytosterols was carried out using the methodology described by Miras-Moreno et al [18]. Briefly, dried broccoli by-products were incubated with methanol:Tris-HCl (50 mM, pH 7.5) (1:1; v/v) in an ice bath and in darkness for 20 min at 4 • C. Then, chloroform (1:1, v/v) was added and the samples were centrifugated at 15.000× g for 5 min.…”

Section: Conventional Extractionmentioning

confidence: 99%

Revalorization of Broccoli By-Products for Cosmetic Uses Using Supercritical Fluid Extraction

et al. 2020

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The agri-food industry is currently one of the main engines of economic development worldwide. The region of Murcia is a reference area in Europe for the cultivation of fruits and vegetables and produces the bulk of Spanish exports of broccoli (Brassica oleracea var. italica). The processing of fresh produce generates a huge number of by-products that represent an important economic and environmental problem when discarded. In this work, an advanced extraction technique using environmentally friendly solvents was applied to assess the revalorization of broccoli by-products, by performing a comparative analysis with conventional extraction. To achieve this goal, supercritical fluid extraction based on response surface methodology was performed using CO2 and ethanol as solvents. The results obtained showed that the supercritical fluid extracts were rich in β-carotene, phenolic compounds, chlorophylls and phytosterols. Moreover, in bioactivity assays, the supercritical fluid extracts exhibited a high antioxidant activity and a cytoprotective effect in a non-tumorigenic keratinocyte cell line exposed to ultraviolet B light. The results indicate that supercritical fluid extracts from broccoli by-products could potentially serve as an ingredient for cosmetic purposes.

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Enhanced accumulation of phytosterols and phenolic compounds in cyclodextrin-elicited cell suspension culture of Daucus carota

Cited by 23 publications

References 55 publications

Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

The Sterol Carrier Hydroxypropyl-β-Cyclodextrin Enhances the Metabolism of Phytosterols by Mycobacterium neoaurum

Revalorization of Broccoli By-Products for Cosmetic Uses Using Supercritical Fluid Extraction

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