Phospholipid Profiles of Neoplastic Human Breast Tissues

Merchant, T.E.; Gierke, L. W.; Meneses, Patricio; Glonek, Thomas

doi:10.1080/10426509008040931

Cited by 2 publications

(3 citation statements)

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“…This elevation is confirmed in the complex contrast of malignant tissue against nonmalignant tissue which adds to a previous finding (Merchant et al, 1988) where the chemical residues of phosphatidylethanolamine, phosphorylethanolamine, glycerol 3-phosphorylethanolamine and a-glycerolphosphate were significantly elevated in malignant human breast tissues compared with benign and noninvolved tissues. This constellation implies that all the metabolites in phosphatidylethanolamine metabolism are elevated and is confirmed by the important role that PE has been found to play in the modification of membrane shape in malignant tissues (Cullis et al, 1985).…”

Section: Discussionsupporting

confidence: 72%

“…These two principle precursor-products of phospholipid metabolism can be measured in analytical MR studies of perchloric acid extracts of human breast tissues (Merchant et al, 1988). These same two metabolites also contribute the majority of the signal detected in the phosphomonoester spectral region of intact cells and of in vivo MR analysed human breast tissues (Ronen et al, 1988;Sijens et al, 1988).…”

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31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

Merchant

Meneses²,

Gierke³

et al. 1991

Br J Cancer

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Phospholipids from malignant, benign and noninvolved human breast tissues were extracted by chloroform-methanol (2:1) and analysed by 31P MR spectroscopy at 202.4 MHz. Thirteen phospholipids were identified as constituents of the profiles obtained among the 55 tissue specimens analysed. Observed patterns in phospholipid tissues profiles were distinct, allowing qualitative characterisation of the three tissue groups. Multivariate analysis of lysophosphatidylcholine (LPC) and an uncharacterised phospholipid were shown to be independently significant in predicting benign tissue histology as either fibrocystic disease or fibroadenoma in 92% of cases. Univariate analysis of relative mole-percentage of phosphorus concentrations of individual phospholipids using the ScheffĂ© comparison procedure revealed that in malignant tissues, phosphatidylethanolamine was significantly elevated compared to benign (+ 32%) and noninvolved tissues (+ 22%). Phosphatidylinositol (+ 33%) and phosphatidylcholine plasmalogen (PC plas) (+ 25%) were increased in malignant compared to benign and LPC was decreased (-44%) in malignant compared to noninvolved. LPC was significantly depressed (-39%) in benign tissue compared to normal. Phospholipid indices computed to further characterise the three tissue groups showed PC plas/PC elevated in malignant tissue compared to benign and PE plas/PE depressed in malignant tissue compared to noninvolved. These findings support previous investigations reporting that the alkyl-phospholipid analogues of phosphatidylcholine are released by malignant tissues and that levels of ethanolamine are elevated in malignant tissues. Indices describing the choline-containing phospholipids showed that these lipids are depressed significantly in malignant tissue relative to healthy tissue.

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Section: Discussionsupporting

confidence: 72%

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confidence: 99%

31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

Merchant

Meneses²,

Gierke³

et al. 1991

Br J Cancer

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“…Using homogenates of biopsy specimens, researchers have shown that these findings can be exploited for the diagnosis of different diseases in humans (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). In muscle tissue homogenates, NMR spectroscopy has shown different concentrations of various metabolites in different muscular dystrophies (6-7).…”

Section: Introductionmentioning

confidence: 99%

Novel corrective equations for complete estimation of human tissue lipids after their partial destruction by perchloric acid pre‐treatment: high‐resolution ¹H‐NMR‐based study

et al. 2008

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Owing to the small quantity of tissue available in human biopsy specimens, aqueous and lipid components often have to be determined in the same tissue sample. Perchloric acid (PCA) used for the extraction of aqueous metabolites has a deleterious effect on lipid components; the severity of the damage is not known. In this study, human muscle tissue was first treated with PCA to extract aqueous metabolites, and the residue was then used for lipid extraction by conventional methods, i.e. the methods of Folch and Bligh & Dyer and a standardised one using methanol/chloroform (1:3, v/v) used in our laboratory. A (1)H-NMR spectrum was obtained for each lipid extract. Lipid was quantified by measuring the integral area of N(+)-(CH(3))(3) signals of phospholipids (PLs). Triacylglycerol (TG) and cholesterol (CHOL) were quantified using the -CH(2)- signals of glycerol and the C18 methyl signal, respectively. This study shows that prior use of PCA caused marked attenuation of TG, PL, and CHOL. This was confirmed by recovery experiments and observation of the direct effect of PCA on the standard lipid components. On the basis of the quantity of lipid lost in each case, three novel equations (with respect to TG, PL, and CHOL) were derived. Application of these equations to lipid quantities estimated in different pathological tissues after PCA pre-treatment produced values equivalent to those estimated without PCA use. This study conclusively shows that PCA pre-treatment damages all three lipid moieties, TG, PL, and CHOL. When PCA is used in a fixed ratio to the tissue, the lipid damage is also proportional and correctable by statistically derived equations. These equations will be useful in human biopsy specimens where aqueous and lipid components have to be studied using the same tissue sample because of the small quantity available.

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Phospholipid Profiles of Neoplastic Human Breast Tissues

Cited by 2 publications

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31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

Novel corrective equations for complete estimation of human tissue lipids after their partial destruction by perchloric acid pre‐treatment: high‐resolution ¹H‐NMR‐based study

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Phospholipid Profiles of Neoplastic Human Breast Tissues

Cited by 2 publications

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31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

31P Magnetic resonance phospholipid profiles of neoplastic human breast tissues

Novel corrective equations for complete estimation of human tissue lipids after their partial destruction by perchloric acid pre‐treatment: high‐resolution 1H‐NMR‐based study

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Novel corrective equations for complete estimation of human tissue lipids after their partial destruction by perchloric acid pre‐treatment: high‐resolution ¹H‐NMR‐based study