Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data

Soil microbial diversity and community composition during conversion from conventional to organic agriculture

“…It will also be helpful if future work shifts from using semi‐quantitative meta‐barcoding methods (Gloor et al, 2017; Morton et al, 2019) to quantitative approaches, for example by including microbial biomass obtained from PLFA analysis. This will allow to test how the actual abundance or biomass of certain microorganisms responds to changes in agricultural management (Lewe et al, 2021; Morton et al, 2019).…”

Evaluation of Biostimulation, Bioaugmentation, and Organic Amendments Application on the Bioremediation of Recalcitrant Hydrocarbons of Soil

“…PLFAs were used as valuable viable or active microbial biomass biomarkers in our study. These are membrane lipids rapidly metabolized and decomposed outside the cell, as demonstrated by (Lewe et al, 2021). The functional adaptation of the soil microbial community reflected in the dynamics of individual hydrocarbons was mirrored by structural adaptation reflected in PLFA dynamics expressed by a site-specific PFLA ratio (Narendrula-Kotha and Nkongolo, 2017).…”

Spatial Variability in Streambed Microbial Community Structure across Two Watersheds

“…Our findings highlight that variations in microbial community structures within streams reflect a mosaic of small-scale patches and suggest that the type and spatial arrangement of patches is an important and often overlooked component of studies of metacommunity ecology of fluvial networks. Lastly, the fact that bacteria in headwater streams are typically components of a mixed-phylum community ( 83 ) and a growing literature that documents the impacts of microeukaryotes on microbial community structure underscore a need to place research on bacterial community structure based on molecular methods into a broader phylogenetic context ( 84 ).…”