Phospholipid Fatty Acid Composition and Diamine Oxidase Activity of Intestinal Mucosa From Rats Treated With Irinotecan Hydrochloride (CPT‐11) under Vegetable Oil–Enriched Diets: Comparison Between Perilla Oil and Corn Oil

Usami, Makoto; Ohata, Atsushi; Kishimoto, Kazunori; Ohmae, Keiko; Aoyama, Masayuki; Miyoshi, Makoto; Fueda, Yuri

doi:10.1177/0148607106030002124

Cited by 7 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When intestinal mucosal epidermis is damaged, DAO is released into blood, indicating that DAO in blood reflects the destruction of the intestinal mucosal epithelial cell layer and intestinal mucosa barricade [14,15] . In our study, DAO was localized mostly in the epithelial cell layer of mucous membrane as previously described [16] , and Lianshu preparation could effectively decrease DAO induced by LPS. However, its mechanism of action remains unclear.…”

supporting

confidence: 74%

Effect of Lianshu preparation on lipopolysaccharide-induced diarrhea in rats

Li¹,

Wan²,

Xu³

et al. 2009

WJG

View full text Add to dashboard Cite

AIM:To investigate the effect of Lianshu preparation on lipopolysaccharide (LPS)-induced diarrhea in rats. METHODS: A diarrhea model was established inSprague Dawley rats via injection of 1 mL of 30 mg/kg LPS. A total of 40 rats were randomly divided into normal group, LPS group, LPS + Lianshu group, LPS + berberine group (n = 10 in each group). Their intestinal mucosal barrier and frequency of diarrhea were observed. Levels of glucose, serum Na + , K + , Cl -and hematocrit, plasma nitrogen monoxide (NO), diamine oxidase (DAO), and D (-)-lactate were measured. The number of IgA+ plasma cells in small intestine was detected and SIgA levels in the intestinal fluid were measured. The antipyretic activity of Lianshu preparation in rats was evaluated using Brewer's yeast-induced pyrexia (10 mL/kg of 20% aqueous suspension). Acetaminophen (250 mg/kg, intragastric administration, bid ) was used as a standard drug for comparison. Temperature was recorded 1 h before and 6 h after Brewer's yeast injection. Finally, small intestinal transmission in mice treated with Lianshu was detected after intraperitoneal injection of methyl prostigmin (2 mg/kg). Atropine (10 g/kg) was used as a control. The ink content in intestine was determined and the total length of intestine was measured. RESULTS:The frequency of diarrhea was higher in LPS group than in LPS + Lianshu group and LPS + berberine group (36.70 ± 5.23 vs 28.50 ± 4.06 and 32.70 ± 9.30 respectively, P < 0.01), and lower in LPS + Lianshu group than in LPS + berberine group (P = 0.03). The levels of Na + , glucose, Cl -, K + were significantly lower in LPS + Lianshu group than in LPS + berberine group and D (-)-lactate were higher in LPS group than in normal group (79.74 ± 7.39 μmol/L vs 24.94 ± 3.38 μmol/L, 2.48 ± 0.42 μ/mL vs 0.82 ± 0.33 μ/mL, 5.63 ± 0.85 μg/mL vs 2.01 ± 0.32 μg/mL respectively, P < 0.01), and lower in LPS + Lianshu group than in LPS + berberine group (48.59 ± 4.70 μmol/L vs 51.56 ± 8.38 μmol/L, 1.43 ± 0.53 μmol/mL vs 1.81 ± 0.42 μmol/mL, 4.00 ± 0.54 μg/mL vs 4.88 ± 0.77 μg/mL respectively, P < 0.05). The morphology of the intestinal mucosa showed destroyed villi in LPS group and atrophied intestinal mucosa in other groups. The pathological intestinal mucosal changes were less in LPS + Lianshu group than in LPS group. The number of IgA+ plasma cells and amount of SIgA were higher in LPS + Lianshu group than in LPS group (1.16 ± 0.19/μm 2 vs 1.09 ± 0.28/μm 2 , P = 0.026; 0.59 ± 0.12 mg/L vs 0.15 ± 0.19 mg/L respectively, P = 0.000).Lianshu had counteractive effects on yeast-induced pyrexia and enterokinesia in rats. CONCLUSION:Lianshu preparation has therapeutic effects on LPS-induced diarrhea and enterokinesia in rats.

show abstract

supporting

confidence: 74%

Effect of Lianshu preparation on lipopolysaccharide-induced diarrhea in rats

Li¹,

Wan²,

Xu³

et al. 2009

WJG

View full text Add to dashboard Cite

show abstract

“…Other reports have also shown that supplementation with ALA-rich perilla oil resulted in a significant increase of ALA and EPA in the colon mucosa of rats treated with CPT-11, an anticancer drug (17). Whereas conversion of ALA into EPA and DHA is still greatly debated in humans (34), a previous study has shown that supplementation with camelina oil significantly increased serum ALA, EPA, and DHA concentrations in hypercholesterolemic patients (35).…”

Section: Discussionmentioning

confidence: 93%

“…Rats received either an (n-3) PUFA diet (TNBS+ALA group, 450 mg×kg 21 ×d 21 ) or corn oil diet (control and TNBS groups). The diets were isocaloric and corn oil was chosen as a control diet because of its current use in many studies using dietary ALA-rich oils such as flaxseed oil (16) or perilla oil (17). Rats were feed deprived for 24 h prior to induction of colitis and were allowed free access to tap water throughout the study.…”

Section: Dietmentioning

confidence: 99%

An α-Linolenic Acid-Rich Formula Reduces Oxidative Stress and Inflammation by Regulating NF-κB in Rats with TNBS-Induced Colitis ,

Hassan¹,

Ibrahim²,

Mbodji³

et al. 2010

The Journal of Nutrition

145

110

View full text Add to dashboard Cite

We have previously shown that α-linolenic acid (ALA), a (n-3) PUFA exerts in vitro antiinflammatory effects in the intestine. In this study, we aimed to evaluate its effect on inflammatory and oxidative stress in a colitis model. Colitis was induced in 2 groups at d 0 by intrarectal injection of 2-4-6-trinitrobenzen sulfonic acid (TNBS), whereas the control group received the vehicle. Rats we fed 450 mg . kg(-1) . d(-1) of ALA (TNBS+ALA) while the other colitic group (TNBS) and the control group were fed an isocaloric corn oil formula for 14 d (from d -7 to d 7). RBC fatty acid composition was assessed. Oxidative stress was studied by measuring urinary 8-isoprostanes (8-IP) and colon glutathione (GSH) concentration and inducible nitric oxide synthase (iNOS) expression. Colitis was assessed histologically, by production of proinflammatory mediators, including cytokines, leukotrienes B(4) (LTB(4)), and cyclooxygenase-2 (COX-2) and by nuclear factor-κB (NF-κB) activation. The ALA-rich diet significantly increased the RBC levels of ALA, eicosapentaenoic acid, and docosapentaenoic acid (n-3) compared with the TNBS group (P < 0.01 for all). The beneficial effect of ALA supplementation on oxidative stress was reflected by lower urinary 8-IP levels (P < 0.05), a normalized colon GSH concentration (P < 0.01), and reduced colon iNOS expression (P < 0.05) compared with the TNBS group. ALA also protected against colon inflammation as assessed by lower tumor necrosis factor-α secretion and mRNA level (P < 0.05), reduced NF-κB activation (P = 0.01), and lower colon lipid mediator concentrations such as LTB(4) and COX-2 (P < 0.05) compared with the TNBS group. These findings show that an ALA-rich formula is beneficial to TNBS-induced colitic rats via inhibition of oxidative and inflammatory stress.

show abstract

“…The enzyme reaction was stopped by the addition of 0.5 ml 30 mmol/l sodium diethyldithiocarbamate, and absorbance was measured at 668 nm. 16 Protein concentrations of leptin in the tissue homogenate were measured by enzyme immunosorbent assay (ELISA) kits (code no. 27295; IBL, Gunma, Japan) according to the manufacturer's instructions.…”

Section: Dao Activity and Protein Concentrations Of Leptin In The Smamentioning

confidence: 99%

Medium-chain triglycerides enhance mucous secretion and cell proliferation in the rat

et al. 2009

View full text Add to dashboard Cite

show abstract

Phospholipid Fatty Acid Composition and Diamine Oxidase Activity of Intestinal Mucosa From Rats Treated With Irinotecan Hydrochloride (CPT‐11) under Vegetable Oil–Enriched Diets: Comparison Between Perilla Oil and Corn Oil

Abstract: CPT-11 induced changes of intestinal mucosal fatty acid composition with increased PGE(2) level and decreased intestinal integrity; perilla oil shows the possibility of being able to attenuate those changes.

Cited by 7 publications

References 27 publications

Effect of Lianshu preparation on lipopolysaccharide-induced diarrhea in rats

Effect of Lianshu preparation on lipopolysaccharide-induced diarrhea in rats

An α-Linolenic Acid-Rich Formula Reduces Oxidative Stress and Inflammation by Regulating NF-κB in Rats with TNBS-Induced Colitis ,

Medium-chain triglycerides enhance mucous secretion and cell proliferation in the rat

Contact Info

Product

Resources

About