Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including australian marsupials; Quantitative 31P NMR Analysis using detergent

Nouri-Sorkhabi, M. Hossein; Agar, Nicholas; Sullivan, David; Gallagher, C. H.; Kuchel, Philip W.

doi:10.1016/0305-0491(95)02011-x

Cited by 64 publications

(32 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to give useful reports on a species that is a well-known animal model, a study has been carried out on pig plasma and erythrocyte membrane phospholipids. The results are compared with those measured, using the same method, in the horse (Nouri-Sorkhabi et al, 1996;Bruschetta et al 2004) and, only concerning plasma phospholipids, in humans (Süllentrop et al 2002).…”

Section: Introductionmentioning

confidence: 95%

“…Interspecies differences in the lipid composition of erythrocyte membranes (Nelson 1967) among mammals have been detected by time-consuming multi-step methods, which are not always accurate, because phospholipid recoveries are influenced by the conditions of extraction (Eder et al 1993). By means of 31 P NMR analysis using detergents it has been possible, using a one single step method, to indicate differences in plasma and erythrocyte membrane phospholipids between mammals and marsupials (Nouri-Sorkhabi et al 1996), changes in the composition and quantitative distribution of pig erythrocyte membrane phospholipids, as compared with other species, and the significantly higher levels of plasma phosphatidylcholine (PC) and sphingomyelin (SPH) in the chicken (Bruschetta et al 2004). In order to give useful reports on a species that is a well-known animal model, a study has been carried out on pig plasma and erythrocyte membrane phospholipids.…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Characterization of phospholipid composition of pig plasma and erythrocyte membranes

et al. 2008

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Characterization of phospholipid composition of pig plasma and erythrocyte membranes

et al. 2008

View full text Add to dashboard Cite

“…Phosphatidylethanolamine (PE), for example, accounts for 33.3% of human erythrocyte membrane mass, but composes 47.3% and 67.8% of goat and sheep erythrocytes, respectively (34). Moreover, PC is absent in goat and sheep erythrocyte membranes, but makes up 30.3% of the lipid composition in humans (34,35). Can these variations in membrane lipid composition account for the difference in PG-1 activity observed in the various red blood cells?…”

mentioning

confidence: 99%

Interaction of antimicrobial peptide protegrin with biomembranes

Gidalevitz

Ishitsuka

Muresan

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

210

177

View full text Add to dashboard Cite

The antimicrobial peptide protegrin-1 (PG-1) interacts with membranes in a manner that strongly depends on membrane lipid composition. In this research we use an approach representing the outer layers of bacterial and red blood cell membranes with lipid monolayers and using a combination of insertion assay, epifluorescence microscopy, and surface x-ray scattering to gain a better understanding of antimicrobial peptide's mechanism of action. We find that PG-1 inserts readily into anionic dipalmitoyl-phosphatidylglycerol, palmitoyl-oleoyl-phosphatidylglycerol, and lipid A films, but significantly less so into zwitterionic dipalmitoyl-phosphatidylcholine, palmitoyl-oleoyl-phosphatidylcholine, and dipalmitoyl-phosphatidylethanolamine monolayers under similar experimental conditions. Epifluorescence microscopy shows that the insertion of PG-1 into the lipid layer results in the disordering of lipid packing; this disordering effect is corroborated by grazing incidence x-ray diffraction data. X-ray reflectivity measurements further point to the location of the peptide in the lipid matrix. In a pathologically relevant example we show that PG-1 completely destabilizes monolayer composed of lipid A, the major component in the outer membrane of Gram-negative bacteria, which is likely to be the mechanism by which PG-1 disrupts the outer membrane, thus allowing it to reach the target inner membrane.

show abstract

“…Quantitative analysis of the concentrations of phospholipids was performed as previously described (17)(18)(19)(20).…”

Section: Methodsmentioning

confidence: 99%

Parallel Secretion of Pancreatic Phospholipase A2, Phospholipase A1, Lipase, and Colipase in Children with Exocrine Pancreatic Dysfunction

Nouri-Sorkhabi¹,

Chapman

Kuchel

et al. 2000

Pediatr Res

Self Cite

View full text Add to dashboard Cite

The cosecretion of pancreatic lipase and colipase are important in normal fat digestion. As adsorption of phosphatidylcholine to the lipid substrate interferes with lipase activity, hydrolysis to lysophosphatidylcholine with subsequent desorption is also essential for fat digestion. There are some data regarding the secretion of pancreatic phospholipases in normal adults but none in children or patients with pancreatic disease. In the present study, we aimed a) to develop an accurate fast assay method to measure phospholipase A 2 and b) to determine the secretion rate of pancreatic phospholipase A 2 and whether it is cosecreted with lipase and colipase in children with exocrine pancreatic dysfunction. Nine male patients aged 0.5 to 16 y (seven with cystic fibrosis, two with malabsorption) underwent pancreatic stimulation tests. Their colipase and lipase secretion rates were measured by titrimetric methods and phospholipase A 2 and A 1 by phosphorus magnetic resonance spectroscopy ( 31 P NMR). It was found that the phospholipases, colipase, and lipase were absent in the two patients with pancreatic insufficiency. In patients with normal absorption, there were marked inter-and intrasubject variations of lipase, colipase, and phospholipase secretion rates that were consistent with the degree of exocrine pancreatic dysfunction. However, in the three 20-min stimulation periods of the pancreatic function test, pancreatic phospholipase is cosecreted with lipase and colipase, and average colipase and phospholipase A 2 secretion rates follow a similar or parallel pattern. These findings are consistent with the important role of pancreatic phospholipases in intestinal phospholipid hydrolysis leading to the desorption of phospholipids from the lipid substrate and enhancing lipid hydrolysis and phospholipid absorption. Abbreviations CEH, carboxyl ester hydrolase CF, cystic fibrosis GPC, glycerophosphocholine lysoPC, lysophosphatidylcholine MCC, mucocutaneous candidiasis and polyendocrinopathy NMR, nuclear magnetic resonance PC, phosphatidylcholine PLA 1 , phospholipase A 1 PLA 2 , phospholipase A 2 PI, pancreatic insufficient PS, pancreatic sufficient Exocrine pancreatic function has been well characterized in children with pancreatic disease with the use of quantitative pancreatic function tests (1-3). To determine the relationship between residual pancreatic function and fat absorption, sensitive titrimetric assays were developed for lipase and the lipase cofactor colipase (4) and their secretion rates compared with fecal fat excretions in children with CF, Shwachman syndrome, or controls (3,5). These studies demonstrated that patients with normal fat absorption had a wide range of enzyme/coenzyme secretion from less than 5% up to within the normal control range; and the threshold for lipolytic failure was near 1% of average control colipase values, i.e. patients with values below this level had fat malabsorption and above this level normal absorption. These observations led to the currently accepted definition that patie...

show abstract

Phospholipid composition of erythrocyte membranes and plasma of mammalian blood including australian marsupials; Quantitative 31P NMR Analysis using detergent

Cited by 64 publications

References 14 publications

Characterization of phospholipid composition of pig plasma and erythrocyte membranes

Characterization of phospholipid composition of pig plasma and erythrocyte membranes

Interaction of antimicrobial peptide protegrin with biomembranes

Parallel Secretion of Pancreatic Phospholipase A2, Phospholipase A1, Lipase, and Colipase in Children with Exocrine Pancreatic Dysfunction

Contact Info

Product

Resources

About