Characterization of phospholipid composition of pig plasma and erythrocyte membranes

Bruschetta, Giuseppe; Alberghina, Daniela; Nastasi, Giancarlo; Rotondo, E.; Ferlazzo, A

doi:10.1007/s11259-008-9101-7

Cited by 2 publications

(5 citation statements)

References 10 publications

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“…Abbreviations used on the spectra: PC phosphatidylcholine; PS phosphatidylserine; LPC lysophosphatidylcholine; PE phosphatidylethanolamine; SPH sphingomyelin; Pi inorganic phosphate; 2,3-DPG 2, 3-diphosphoglycerate. All spectra were scaled independently to yield an identical vertical maximum amplitude of the largest peak L-packed erythrocytes) and significantly higher (P<0.001) than that for untrained and trained horse plasma Pi (3.02±0.68 mmol/L-plasma), in good agreement with our previous study (Bruschetta et al, 2008), and was significantly higher (P<0.001) than chicken plasma Pi (3.44±0.92 mmol/L-plasma). Lastly, the amount of chicken plasma Pi was comparable with horse plasma Pi but lower than ostrich plasma Pi (5.24±1.72 mmol/L-plasma).…”

Section: Resultssupporting

confidence: 88%

“…The results obtained were compared with those previously reported (Bruschetta et al 2008;Nouri-Sorkhabi et al 1996) in the main domestic animal species. The specific erythrocyte [SPH/total PLs] molar ratios,~28% in pigs,~15% in horses,4 1% in cows found confirm interspecies differences in the composition of erythrocyte membrane PLs (Bruschetta et al 2008;Hawthorne and Ansell 1982;Koval and Pagano 1991). The different PC/SPH molar ratios found (~1.0 in pig erythrocyte membranes,~0.1 in cows,~2.2 in horses, Fig.…”

Section: Discussionmentioning

confidence: 81%

“…Literature data on phospholipid composition of plasma and erythrocyte membranes in animal species are rather limited (Bruschetta et al 2008;Engen and Clark 1990;Fuchs et al 2009;Nouri-Sorkhabi et al 1996) and most of all not homogeneous, as they have been assessed with a number of different techniques (HPLC, NMR, MS). Moreover, they have been collected by time-consuming, multi-step methods, which are not always accurate, because the recovery of PLs is influenced by the conditions of extraction.…”

Section: Discussionmentioning

confidence: 99%

“…The present investigation was undertaken with the aim of analyzing changes in the composition and quantitative distribution of plasma and erythrocyte membrane PLs in domestic animal species by a single step method, 31 P NMR analysis using detergents (Bruschetta et al 2008;Nouri-Sorkhabi et al 1996), without any previous PLs extraction by organic solvents (Estrada et al 2008). The use of detergents prevents the formation of large PL micelles in water and, as a consequence, highly resolved resonances of PLs can be detected in 31 P NMR spectra (London and Feigenson, 1979).…”

Section: Introductionmentioning

confidence: 99%

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Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

et al. 2011

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The aim of this study was to provide basal values of phospholipid (PL) composition in different animal species by 31P NMR analysis using detergents. This fast and accurate method allowed a quantitative analysis of PLs without any previous separation. Plasma and erythrocyte membrane PLs were investigated in mammals (pig, cow, horse). Moreover, for the first time, the composition of plasma PLs in avian (chicken and ostrich) was performed by 31P NMR. Significant qualitative and quantitative interspecies differences in plasma PL levels were found. Phosphatidilcholine (PC) and sphingomyelin (SPH) levels were significantly higher (P < 0.001) in chicken plasma than all the other species tested. In erythrocytes, cow PC and phosphatidylcholine diarachidoyl were significantly lower (P < 0.001) than for pigs and horses, whereas pig PC presented intermediate values among cows and horses. Inorganic phosphate and 2,3-diphosphoglycerate levels were also significantly different between the species under investigation. The [SPH/total PLs] molar ratios in erythrocytes confirmed interspecies differences in phospholipid composition while the PC/SPH molar ratios could be related to a distinct erythrocyte flexibility and aggregability. Diet and nutrition may contribute primarily to the interspecies differences in plasma PL amounts detected. Significant differences between chicken plasma PC and SPH levels and those of the other animal species could be ascribed to a fat metabolism specific to egg production.

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Section: Resultssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

et al. 2011

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“…Polar lipid class quantification revealed that the dolphin plasma has comparable PtdCho content, remarkably lower CerPCho content, and higher LPC when compared with human plasma (Bruschetta et al, 2008; Dougherty et al, 1987; Hodson et al, 2008; Phillips & Dodge, 1967; Williams et al, 1966). Moreover, taking into account what is known for other terrestrial mammals, PtdCho is always the major phospholipid present in plasma, with these dolphins being in the group of mammals where PtdCho content is higher (Bruschetta et al, 2008). A previous study in T. truncatus plasma underestimated PtdCho and reported a higher total of CerPCho (Nelson, 1973).…”

Section: Discussionmentioning

confidence: 99%

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

Monteiro

Maciel

Melo

et al. 2021

Lipids

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Plasma biochemical analysis remains one of the established ways of monitoring captive marine mammal health. More recently, complementary plasma lipidomic analysis has proven to be a valid tool in disease diagnosis and prevention, with the potential to validate and complement common biochemical analysis, providing a more integrative approach. In this study, we thoroughly characterized the plasma polar lipid content of Tursiops truncatus, the most common cetacean species held under human care. Our results showed that phosphatidylcholine, lysophosphatidylcholine, and sphingomyelins (CerPCho) are the most represented phospholipid classes in T. truncatus plasma. Palmitic, oleic, and stearic acids are the major fatty acid (FA) present esterified to the plasma polar lipids of this species, although some n-3 species are also remarkably present, namely eicosapentaenoic and docosahexaenoic acids. The polar lipidome identified by HILIC LC-MS allowed identifying 304 different lipid species. These species belong to the phosphatidylcholine (103 lipid species), lysophosphatidylcholine (35), phosphatidylethanolamine (71), lysophosphatidylethanolamine (20), phosphatidylglycerol (13), lysophosphatidylglycerol (5), phosphatidylinositol (15), lysophosphatidylinositol (3), phosphatidylserine (6) lysophosphatidylserine (1), and sphimgomyelin (32) classes. This was the first time that the dolphin plasma phospholipid profile was characterized, providing a knowledge that will be important to further understand lipid metabolism and physiological regulation in small cetaceans. Furthermore, this study proved the practicability of the use of plasma lipid profiling for health assessment in marine mammals under human care.

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Characterization of phospholipid composition of pig plasma and erythrocyte membranes

Cited by 2 publications

References 10 publications

Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

Phospholipid composition of plasma and erythrocyte membranes in animal species by 31P NMR

The plasma phospholipidome of Tursiops truncatus: From physiological insight to the design of prospective tools for managed cetacean monitorization

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