Phospholipid-Cholesterol Membrane Model

Tobias, Julian M.; Agin, D.; Pawlowski, Raymond

doi:10.1085/jgp.45.5.989

Cited by 119 publications

(15 citation statements)

References 19 publications

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“…The results reported here are in agreement with the findings of othe: authors who have studied the interaction of calcium ions, either witt fragments of natural biological membranes [5,20] or with artificial phos pholipid membranes [23][24][25]28]. In both cases, the interaction has beeI shown to be pH dependent.…”

Section: Discussionsupporting

confidence: 92%

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Effect of calcium deprivation on frog skeletal muscles at different pH values

Argibay

Caputo

1971

J. Membrain Biol.

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Whole sartorius muscles from the frog were exposed to calcium-free solutions at different pH values. The depolarizations caused by these solutions were measured 1 hr after the solution change. When the pH was 7.2, the mean depolarization was 8 mV. At pH values of 5.1 and 8.5, the mean depolarizations were 1 and 17 mV, respectively. Similar experiments were carried out with solutions in which the main anion was sulfate instead of chloride. In these cases, the depolarization values caused by calcium deprivation at pH values of 7.2, 5.5, and 8.5 were 11, 4, and 20 mV, respectively.

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Section: Discussionsupporting

confidence: 92%

“…This behavior is rather similar to that of phospholipid monolayers [23][24][25]28]. Some years ago, Straub [27] reported that the depolarization of frog myelinated nerve fiber membrane, caused by calcium deprivation, was increased by alkaline and decreased by acid pH.…”

supporting

confidence: 63%

Effect of calcium deprivation on frog skeletal muscles at different pH values

Argibay

Caputo

1971

J. Membrain Biol.

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“…Studies described in this series of papers have been undertaken along this line of thought. A similar attempt was discussed by Tobias, Agin and Pawlowski [10] some years ago.…”

mentioning

confidence: 73%

Transport phenomena in a model membrane accompanying a conformational change: Membrane potential and ion permeability

1972

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Summary. Membrane potential A ~0,n ion permeability P and d-c conductance G ~ of a model membrane composed of a filter paper and a synthetic lipid analogue, i.e. dioleylphosphate (DOPH), were observed in various concentrations of t : 1 type electrolyte solution. With successive increases of salt concentration in the external solution, d ~Om, P and G ~ changed their values rather discontinuously at a certain critical value of the concentration c t. When the concentration was decreased successively, the values of A ~om, P and G ~ were returned to the original levels at a concentration much lower than c t. Thus, the transport phenomena observed across the membrane showed an appreciable hysteresis loop.The discontinuous variations of A ~Om, P and G ~ were interpreted in terms of a transition of DOPH adsorbed in the filter paper from an oil membrane to a charged membrane. This transformation of DOPH at the critical salt concentration is consistent with the previous argument that the DOPH impregnated in the filter paper changes its conformation from oil droplets to a number of bilayer films, which deduced from a theoretical analysis of frequency dependency of the electrical capacitance of the membrane.Recent electrochemical studies on squid giant axons suggest that the process of excitation of nervous tissues is accompanied by a conformational change of the macromolecules constituting the membrane, triggered by cooperative cation exchange at fixed negative sites in the membrane [3,7,8,9]. Changes in thermal and optical properties of nervous tissues during excitation support this argument [1,2]. There is serious ambiguity, however in interpreting the results of physical measurements carried out on nerves, since there are many undefinable quantities in a system consisting of an excitable membrane and its natural environment, and since the molecular * Permanent address: Tokyo Institute of Technology, Meguro, Tokyo, Japan.

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“…In addition, MacFarlane & Knight (1941) reported that it possesses phospholipase C activity. The toxin can hydrolyze various phospholipids such as phosphatidylcholine in biological membranes (Tobias et al, 1962; Lenard & Singer, 1968;McILwain & Rapport, 1971;Knickelbein & Rosenberg, 1980). Several workers have reported various biological properties of the crude or the partially purified toxin, especially the experimental production of lesions by administration to various tissues of the crude or partially purified alpha toxin preparation (Strunk et al, 1967;Albuquerque & Thesleff, 1967;Lodge & Leach, 1973;Knickelbein & Rosenberg, 1980).…”

Section: Introductionmentioning

confidence: 99%

Excitatory effect of Clostridium perfringens alpha toxin on the rat isolated aorta

Fujii

Nomura

Oshita

et al. 1986

British J Pharmacology

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Clostridium perfringens alpha toxin caused contraction of the isolated aorta of the rat in a dose‐dependent manner. The contractile action caused by the toxin was inhibited or abolished by calcium antagonists such as nifedipine, verapamil and cinnarizine, or a Ca‐free medium, but was not affected by phentolamine, chlorpheniramine, atropine, tetrodotoxin or a low Na medium. The toxin stimulated Ca uptake into the aorta in a dose‐dependent manner. 8‐N,N'‐diethylaminooctyl‐3,4,5‐trimethoxybenzoate (TMB‐8) blocked significantly both the toxin‐ and noradrenaline (NA)‐induced contractions. Trifluoperazine (TFP) and N‐(6‐aminohexyl)‐5‐chloro‐l‐naphtharene sulphonamide (W‐7) did not affect the contractile activity of the toxin but blocked the NA‐induced contraction. The toxin also stimulated the 32P phosphate labelling of phosphatidylinositol (PI) and phosphatidic acid (PA) in the preparation. These results indicate that the toxin‐induced contraction, which is different from that induced by NA, is the result of a direct action of the toxin on the aorta and is due to an increased Ca2+ permeability across the smooth muscle membrane. It is suggested that the contractile response to the toxin is associated with activation of phospholipid metabolism and enhanced entry of Ca into the aorta.

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Phospholipid-Cholesterol Membrane Model

Cited by 119 publications

References 19 publications

Effect of calcium deprivation on frog skeletal muscles at different pH values

Effect of calcium deprivation on frog skeletal muscles at different pH values

Transport phenomena in a model membrane accompanying a conformational change: Membrane potential and ion permeability

Excitatory effect of Clostridium perfringens alpha toxin on the rat isolated aorta

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