Phospholipid and protein contents of lens proteolipids in human senile cataract

Abstract

Purpose This experimental study was conducted with the aim of evaluating the quantitative change, if any, in the phospholipids and protein contents of lens proteolipids during cataract formation, and to establish whether the phospholipid/protein content of the blood is related to cataractogenesis. Methods A total of 120 human lenses were collected. Phospholipids and protein content in proteolipids of these lenses were estimated biochemically. Blood was examined from total 60 persons. Data obtained were analyzed with the help of the ANOVA program of SPSS software (version 7.5). Results A significant (Po0.01) reduction of proteolipid-bound phospholipids was observed in cataracts as compared with the control lenses. An alteration was also noticed in the protein content of proteolipids in the cataractous lenses. Changes were also observed in serum total phospholipids (Po0.01) and total protein (Po0.01) in cataractous patients. Conclusions As proteolipids are the main constituents of the membranes of lens fibres, the alterations in its phospholipid and protein moieties may be suggestive of the disintegration of lens membranes, which ultimately leads to cataract formation. Gradual and constant variation in serum parameters may be one of the predisposing factors in cataractogenesis.

Protein profiles in cortical and nuclear regions of aged human donor lenses: A confocal Raman microspectroscopic and imaging study

“…It can be opposed that the absence of marked changes is due to insufficiency to visualize small objects alongside the omnipresent protein spectrum that dominates the Raman spectrum. In the lens the overall phospholipid and cholesterol content is less than 5% of dry weight (Li et al, 1985;Siddique et al, 2010). The small red and orange regions in Fig.…”

Effect of standardized fruit extract of Luffa cylindrica on oxidative stress markers in hydrogen peroxide induced cataract

“…[ 18 ] The perfect physicochemical arrangement of the lens proteins gives transparency to the lens. [ 19 ] Protein aggregation increases with age. The crystallins, which constitute approximately 90% of the TPC of the lens, accumulate, and show many age-related oxidative changes.…”

Overview of Congenital, Senile and Metabolic Cataract

“…The major fatty acids were C160, C24:l and C24:0, and monounsaturated fatty acids accounted for 40-55% the total fatty acids [74]. Siddique et al reported alterations of phospholipid and protein moieties in lens fibers, disrupting function and leading to cataract formation (Figure 3) [75].…”