Phospholipases in action during plant defense signaling

Canonne, Joanne; Froidure-Nicolas, Solène; Rivas, Susana

doi:10.4161/psb.6.1.14037

Cited by 134 publications

(111 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The third group contains four paralogous genes coding for low-molecular-mass PLA 2 s of around 14 kD, sPLA 2 a, sPLA 2 -b, sPLA 2 -g, and sPLA 2 -d Matos and Pham-Thi, 2009). Evidence for the involvement of sPLA 2 in signaling and pathogen defense is more limited (Munnik and Testerink, 2009;Canonne et al, 2011). Their involvement in membrane trafficking, however, is emerging: reductions in sPLA 2 expression in Arabidopsis (Arabidopsis thaliana) plants led to Golgi disruption and the inhibition of PIN auxin efflux transporter traffic to the plasma membrane in roots (Lee et al, 2010) and also to membrane disruption in pollen (Kim et al, 2011).…”

mentioning

confidence: 99%

“…They are involved in several biological processes in both animals and plants (Chen et al, 2011): (1) as a component of the acylation/ hydrolysis of phospholipids in the so-called Lands cycle involved in primary lipid metabolism in animals (Lands, 1960;Balsinde and Dennis, 1997;Imae et al, 2010) and plants (Li et al, 2013); (2) in membrane architecture and trafficking (Lee et al, 2010;Kim et al, 2011); (3) in the production of bioactive compounds involved in signaling, pathogen defense, and programmed cell death (Munnik and Testerink, 2009;Canonne et al, 2011); (4) in catalyzing the hydrolysis of oxidized lipids (van Kuijk et al, 1987;Banas et al, 1992); (5) in seed storage lipid mobilization (Rudolph et al, 2011);and (6) in uncommon and modified FA production in seeds (Bafor et al, 1991;Ståhl et al, 1995;Bates and Browse, 2011).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Small Phospholipase A2-α from Castor Catalyzes the Removal of Hydroxy Fatty Acids from Phosphatidylcholine in Transgenic Arabidopsis Seeds

et al. 2015

View full text Add to dashboard Cite

Ricinoleic acid, an industrially useful hydroxy fatty acid (HFA), only accumulates to high levels in the triacylglycerol fraction of castor (Ricinus communis) endosperm, even though it is synthesized on the membrane lipid phosphatidylcholine (PC) from an oleoyl ester. The acyl chains of PC undergo intense remodeling through the process of acyl editing. The identities of the proteins involved in this process, however, are unknown. A phospholipase A2 (PLA2) is thought to be involved in the acyl-editing process. We show here a role for RcsPLA2α in the acyl editing of HFA esterified to PC. RcsPLA2α was identified by its high relative expression in the castor endosperm transcriptome. Coexpression in Arabidopsis (Arabidopsis thaliana) seeds of RcsPLA2α with the castor fatty acid hydroxylase RcFAH12 led to a dramatic decrease in seed HFA content when compared with RcFAH12 expression alone in both PC and the neutral lipid fraction. The low-HFA trait was heritable and gene dosage dependent, with hemizygous lines showing intermediate HFA levels. The low seed HFA levels suggested that RcsPLA2α functions in vivo as a PLA2 with HFA specificity. Activity assays with yeast (Saccharomyces cerevisiae) microsomes showed a high specificity of RcsPLA2α for ricinoleic acid, superior to that of the endogenous Arabidopsis PLA2α. These results point to RcsPLA2α as a phospholipase involved in acyl editing, adapted to specifically removing HFA from membrane lipids in seeds.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

A Small Phospholipase A2-α from Castor Catalyzes the Removal of Hydroxy Fatty Acids from Phosphatidylcholine in Transgenic Arabidopsis Seeds

et al. 2015

View full text Add to dashboard Cite

show abstract

“…4 The involvement of phospholipases in plant immunity to different stresses is due in part to early activation of signaling events. 5 Phospholipases have been shown to play important roles in resistance to various biotic and abiotic stress conditions. For example, phospholipase A (PLA) produces FFA second messengers and precursors of JA and derivatives, while phospholipase D (PLD) hydrolyzes structural phospholipids such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE) to produce phosphatidic acid (PA) which is associated with various stress responses.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

et al. 2014

View full text Add to dashboard Cite

Phosphatidylinositol-specific phospholipase C2 (PLC2) is a signaling enzyme with hydrolytic activity against membrane-bound phosphoinositides. It catalyzes the cleavage of phosphatidylinositol(4,5)bisphosphate (PtdIns(4,5) P 2 ) into two initial second messengers, myo-inositol-1,4,5-trisphosphate (InsP 3 ) and diacylglycerol (DAG). The former, as well as its fully phosphorylated derivative, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP 6 ), play a major role in calcium signaling events within the cell, while DAG may be used in the regeneration of phospholipids or as a precursor for phosphatidic acid (PA) biosynthesis, an important signaling molecule involved in both biotic and abiotic types of stress tolerance. Overexpression of the gene for Brassica napus phospholipase C2 (BnPLC2) in Brassica napus has been shown to enhance drought tolerance, modulate multiple genes involved in different processes and favorably affect hormonal levels in different tissues. We, therefore, undertook the current study with a view to examining, at the metabolome level, its effect on both abiotic (low temperature) and biotic (stem white rot disease) types of stress in canola. Thus, while transgenic plants exhibited a significant rise in maltose levels and a concomitant elevation in some unsaturated free fatty acids (FFAs), glycerol, and glycerol 3-phosphate under subzero temperatures, they accumulated high levels of raffinose, stachyose and other sugars as well as some flavonoids under acclimatization conditions. Collectively, overexpression of BnPLC2 appears to have triggered different metabolite patterns consistent with its abiotic and, to a limited extent, biotic stress tolerance phenotypes.

show abstract

“…Tanto los ácidos grasos y compuestos derivados, como lípidos de mayor complejidad estructural tales como esfingolípidos y fosfolípidos, son importantes para la respuesta frente a distintos tipos de patógenos (Shah, 2005;Canonne et al, 2011).…”

Section: Respuesta De Las Plantas Frente a Patógenosunclassified

“…Contrariamente, la sobreexpresión de SACPD en berenjena provoca una mayor acumulación de ácidos grasos insaturados, como 16:1, 16:3, y 18:1, y una mayor resistencia frente a Verticillium dahliae (Xing y Chin, 2000 Wang, 2004. Los ácidos grasos pueden afectar también a la respuesta frente a patógenos de forma indirecta. Las oxipilinas son productos de degradación por oxidación de ácidos grasos, y han sido caracterizados por su función señalizadora en defensa (Andreou et al, 2009;Canonne et al, 2011). Dentro de este grupo de compuestos, el ácido jasmónico y sus derivados han sido ampliamente caracterizados como reguladores tanto de respuestas a estrés como del desarrollo (Wasternack y Kombrink, 2010).…”

Section: áCidos Grasos Y Compuestos Derivadosunclassified

Caracterización molecular y funcional del gen PATHOGEN AND CIRCADIAN CONTROLLED 1 (PCC1) en Arabidopsis thaliana.

Moreno¹

View full text Add to dashboard Cite

Phospholipases in action during plant defense signaling

Cited by 134 publications

References 66 publications

A Small Phospholipase A2-α from Castor Catalyzes the Removal of Hydroxy Fatty Acids from Phosphatidylcholine in Transgenic Arabidopsis Seeds

A Small Phospholipase A2-α from Castor Catalyzes the Removal of Hydroxy Fatty Acids from Phosphatidylcholine in Transgenic Arabidopsis Seeds

Metabolomic shifts inBrassica napuslines with enhancedBnPLC2expression impact their response to low temperature stress and plant pathogens

Caracterización molecular y funcional del gen PATHOGEN AND CIRCADIAN CONTROLLED 1 (PCC1) en Arabidopsis thaliana.

Contact Info

Product

Resources

About