Abstract:Phospholipase C-related but catalytically inactive proteins PRIP-1 and -2 are inositol-1,4,5-trisphosphate binding proteins that are encoded by independent genes. Ablation of the genes in mice impairs female fertility, which is manifested by fewer pregnancies, a decreased number of pups, and the decreased and increased secretion of gonadal steroids and gonadotropins, respectively. We investigated the involvement of the PRIPs in fertility, focusing on the ovaries of and double-knock-out (DKO) mice. Multiple cys… Show more
“…The endogenous LH levels of the two groups were without differences within 2–16 h after hCG ( Figure 3 B), while PPOS group had an obviously lower LH level than the control group before hCG trigger (1.96 ± 0.55 vs. 0.59 ± 0.17, p < 0.05, Figure 3 D), although the two groups had the similar LH levels at the beginning of COH ( Figure 3 C). Furthermore, the expression of LH/choriogonadotropin receptor (LHCGR) on the theca cells of preovulatory follicles 32 , 33 , 34 was significantly less in the PPOS group ( Figures 3 E and 3F). Figure 3 The serum progesterone and LH levels after hCG injection within the control and PPOS groups (A and B) The serum progesterone (A) and endogenous LH levels (B) in the PPOS and control groups after hCG injection (A: n = 8 mice in each group; B: n = 5–6 mice in each group).…”
“…The endogenous LH levels of the two groups were without differences within 2–16 h after hCG ( Figure 3 B), while PPOS group had an obviously lower LH level than the control group before hCG trigger (1.96 ± 0.55 vs. 0.59 ± 0.17, p < 0.05, Figure 3 D), although the two groups had the similar LH levels at the beginning of COH ( Figure 3 C). Furthermore, the expression of LH/choriogonadotropin receptor (LHCGR) on the theca cells of preovulatory follicles 32 , 33 , 34 was significantly less in the PPOS group ( Figures 3 E and 3F). Figure 3 The serum progesterone and LH levels after hCG injection within the control and PPOS groups (A and B) The serum progesterone (A) and endogenous LH levels (B) in the PPOS and control groups after hCG injection (A: n = 8 mice in each group; B: n = 5–6 mice in each group).…”
To gain a better knowledge of structural variations (SVs) in Xiang pig, we used next-generation sequencing to analyze the Xiang pigs with larger (XL) or smaller litter sizes (XS). Our analysis yielded 28,040 putative SVs in the Xiang pig. These SVs distributed throughout all of chromosomes. Some functional regions including exons and untranslated regions were less varied than introns and intergenic regions. We detected 4637 and 4119 specific SVs, which contained 1697 and 1582 genes in XL and XS group, respectively. These genes were mainly enriched in the well-known pathways involved in development and reproduction processes. Population validation was carried out on 50 SVs candidates using PCR method in 144 Xiang pig crowds. All of 50 SVs were confirmed by PCR method and 14 SVs were associated with the litter size of Xiang pigs. These results may be helpful for the elucidation of growth and reproduction regulation in Xiang pig.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
