Phospholipase A2 regulation of bovine endometrial (BEND) cell prostaglandin production

Godkin, James D.; Roberts, Mary P.; Elgayyar, Mona; Guan, Wei; Tithof, Patricia K.

doi:10.1186/1477-7827-6-44

Cited by 17 publications

(19 citation statements)

References 33 publications

(57 reference statements)

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“…This is in contrast to observed IFNT effects described in vitro (Asselin et al 1997a, 1997c, Parent et al 2002, Emond et al 2004, Tithof et al 2007, Godkin et al 2008. In addition, the release of all PG examined is strikingly concomitant, making it likely that the general rather than the specific synthases account for the vast increase in PG.…”

Section: Discussioncontrasting

confidence: 52%

“…However, we find clear evidence that there is no difference in PGF 2a /PGE 2 ratio between cyclic and pregnant uteri in vivo. The differences reported might therefore resemble in vitro culture situations in which a) the relative contribution of PG production by the different cell populations (Kim & Fortier 1995, Godkin et al 2008 used did not resemble the actual in vivo situation or b) the exposure of IFNT does not take into account the PG contribution of the embryo.…”

Section: Discussionmentioning

confidence: 95%

“…Moreover, it has been discussed that the relative production of PGE 2 and PGF 2a may be more important than the absolute levels of individual-secreted PG, in the choice between embryo implantation and resumption of the estrous cycle (Asselin et al 1997a). IFNT has been made responsible for the reduced production of PGF 2a relative to PGE 2 evidenced by numerous in vitro studies (Asselin et al 1997a, 1997c, Parent et al 2002, Emond et al 2004, Tithof et al 2007, Godkin et al 2008. However, we find clear evidence that there is no difference in PGF 2a /PGE 2 ratio between cyclic and pregnant uteri in vivo.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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Prostaglandins (PGs) are important regulators of reproductive processes including early embryonic development. We analyzed the most relevant PG in bovine uteri at different preimplantation pregnancy stages when compared with non-pregnant controls. Additionally, endometrium and trophoblast tissues were examined regarding specific enzymes and receptors involved in PG generation and function. Simmental heifers were artificially inseminated or received seminal plasma only. At days 12, 15, or 18, post-estrus uteri were flushed for PG determination by liquid chromatography-tandem mass spectrometry. Endometrium and trophoblast tissues were sampled for RNA extraction and quantitative real-time PCR analysis. At all days and points of time examined, the concentration of 6-keto PGF 1a (stable metabolite of PGI 2 ) was predominant followed by PGF 2a OPGE 2 OPGD 2 zTXB 2 (stable metabolite of TXA 2 ). At days 15 and 18, PG increased from overall low levels at day 12, with a much more pronounced increase during pregnancy. The PGF 2a /PGE 2 ratio was not influenced by status. The highest PG concentration was measured at day 15 with 6-keto PGF 1a (6.4 ng/ml) followed by PGF 2a (1.1 ng/ml) and PGE 2 (0.3 ng/ml). Minor changes in endometrial PG biosynthesis enzymes occurred due to pregnancy. Trophoblasts revealed high transcript abundance of general and specific PG synthases contributing to uterine PG. As PGI 2 and PGF 2a receptors were abundantly expressed by the trophoblast, abundant amounts of PGI 2 and PGF 2a in the uterine lumen point towards an essential role of PG for the developing embryo. High amounts of PG other than PGE 2 in the preimplantation uterus may be essential rather than detrimental for successful reproduction.

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Ulbrich¹,

Schülke²,

Groebner³

et al. 2009

Reproduction

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“…In addition, secreted phosphoprotein (SPP1) acts as an adhesion molecule for implantation in sheep and pigs by stimulating cell‐cell adhesion between the maternal endometrium and conceptus through phosphorylation of focal adhesion kinase and activation of the PI3K/mTOR signaling pathway (Johnson, Burghardt, & Bazer, ). The BEND cell line has served as a model for investigating signal transduction related to uterine prostaglandin production regulated by changes in the protein kinase A (PKA), protein kinase C (PKC), nuclear factor kappa B (NF‐κB), and ERK1/2 MAPK pathways (Godkin, Roberts, Elgayyar, Guan, & Tithof, ; Pru et al, ). Consistent with previous results, CCL23‐induced activation of AKT‐P70S6K‐S6 signaling that activates the ERK1/2, JNK, and P38 MAPK pathways in uterine endometrial epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

C‐C motif chemokine ligand 23 abolishes ER stress‐ and LPS‐induced reduction in proliferation of bovine endometrial epithelial cells

Lim

Bae

Bazer

et al. 2017

Journal Cellular Physiology

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To reduce embryonic losses in domestic animals for economic production of livestock meat and milk, chemokines and their receptors are required for proper implantation and placentation during early pregnancy. In this study, we investigated the effects of C-C-motif chemokine ligand 23 (CCL23) on the proliferation of bovine endometrial (BEND) epithelial cells. CCL23 treatment improved BEND cell proliferation by enhancing PCNA and cyclin D1 expression via activation of the PI3K/AKT and MAPK signaling pathways. In addition, a combination of CCL23 and tunicamycin reversed the ER stress-induced reduction in cell proliferation and the decreased expression of UPR-mediated signaling proteins, including IRE1α, PERK, and ATF6α. Moreover, it regulated the lipopolysaccharide-induced inflammation in BEND cells by inhibiting the expression of pro-inflammatory cytokines (IL-6 and IL-8), and by restoring intracellular Ca levels. These findings demonstrate that CCL23 improves endometrial development and uterine receptivity required for implantation and placentation during early pregnancy.

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“…PLA2 (phospholipase A2) is a member of the PLA2 super‐family that catalyze the release of arachadonic acid from phospholipids, which is the rate‐limiting step in prostaglandin production (Capper and Marshall, 2001; Godkin et al, 2008). Our microarray gene profiling data indicates that PLA2 is down‐regulated on Day 18 of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Gene profiling of inflammatory genes in day 18 endometria from pregnant and non‐pregnant mares

Patterson

Squires

Hansen

et al. 2012

Molecular Reproduction Devel

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Maternal recognition of pregnancy is a physiological process that primarily describes endometrial responses to a conceptus. Recognition of a conceptus prevents the release of prostaglandin F(2α) , thereby ensuring survival of the corpus luteum and continued progesterone production. Exactly how this occurs in the mare is poorly understood. Because prostaglandin F(2α) is a pro-inflammatory hormone, we hypothesized that differential gene expression in the endometrium at the time of maternal recognition reflects an anti-inflammatory event leading to decreased prostaglandin F(2α) secretion. Mares were inseminated, and endometrial biopsies were recovered from pregnant mares on Day 18 post-ovulation. In subsequent estrous cycles, mares were not inseminated and Day 18 post-ovulation endometrial biopsies were collected (non-pregnant control, matched per individual). Endometrial gene expression profiles were examined by screening an Affymetrix equine GeneChip containing probes specific for genes related to inflammatory processes. Microarray analysis revealed 118 genes that were up-regulated and 93 genes that were down-regulated (P < 0.001) at least 1.5-fold in the endometrium of pregnant versus non-pregnant mares. Quantitative, real-time RT-PCR confirmed the microarray results for three up-regulated genes homologous to TSC22D3, PPAPDC2, and KLF6, and three down-regulated genes homologous to ESR1, MARCKSL1, and EPSTI1 (P < 0.05). It is concluded that the presence of the equine embryo induces differential gene expression in the endometrium of Day 18 pregnant mares, and that these genes are associated with inflammatory processes and pathways involving cellular growth and proliferation. The results from this study provide important new insights into endometrial gene expression in response to early equine pregnancy.

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Phospholipase A2 regulation of bovine endometrial (BEND) cell prostaglandin production

Cited by 17 publications

References 33 publications

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

Quantitative characterization of prostaglandins in the uterus of early pregnant cattle

C‐C motif chemokine ligand 23 abolishes ER stress‐ and LPS‐induced reduction in proliferation of bovine endometrial epithelial cells

Gene profiling of inflammatory genes in day 18 endometria from pregnant and non‐pregnant mares

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