Gene profiling of inflammatory genes in day 18 endometria from pregnant and non‐pregnant mares

Patterson, Amanda L.; Squires, E.L.; Hansen, Thomas; Bouma, Gerrit J.; Bruemmer, J.E.

doi:10.1002/mrd.22112

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…Pregnant women are more vulnerable to infections due to hormonal fluctuations (Patterson et al, 2012; Wu et al, 2016). Vaginitis is one of the most prevalent gynaecological diseases in pregnant women (Shrestha et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Prenatal maternal vaginal inflammation increases anxiety and alters HPA axis signalling in adult male mice

Wang

Pei²,

Ridong

et al. 2019

Intl J of Devlp Neuroscience

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Background Maternal infection during pregnancy is known to adversely affect foetal development, but previous studies have rarely investigated the impact of gynaecological diseases during pregnancy on offspring during adulthood. Vaginitis is one of the most prevalent gynaecological diseases during pregnancy. Methods The effect of maternal vaginal inflammation on offspring was simulated by inducing maternal vaginal infection. We performed a transvaginal injection of lipopolysaccharide (LPS) in pregnant mice to induce vaginitis and investigated their offspring by means of behavioural tests and molecular and cellular measurements. Results Behavioural tests revealed that the offspring of mothers transvaginally injected with LPS exhibited sex‐dependent differences. Male offspring showed increased anxiety‐related behaviours, including reduced time exploring the open arm in the elevated plus maze test and light chamber in the light‐dark box test. Serum levels of corticosterone were increased in LPS male offspring, indicating activation of the hypothalamic‐pituitary‐adrenal (HPA) axis. Corticotropin‐releasing hormone (CRH) protein expression and c‐Fos positive cells were increased in the hypothalamic paraventricular nucleus (PVN) in LPS male offspring, which presented with an increased number of microglia. Conclusion This study suggests that prenatal vaginal infection increases anxiety‐like behaviour in male offspring, possibly via activation of the HPA axis.

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Section: Introductionmentioning

confidence: 99%

Prenatal maternal vaginal inflammation increases anxiety and alters HPA axis signalling in adult male mice

Wang

Pei²,

Ridong

et al. 2019

Intl J of Devlp Neuroscience

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show abstract

“…For maternal confounding variables, the expression of genes involved in inflammation and placentation (i.e., CX3CR1 36 , TSC22D3 37 and PTX3 ) 38 were associated with parity, indicating that women who have had a prior pregnancy may respond differently to the presence of invading trophoblast compared to nulliparous women. Adipsin expression, which is shown to be abundant in adipose tissue, was increased in the placentas of women with high BMI at term, and placentas from obese women have been shown to secrete higher levels of adipsin 39 .…”

Section: Discussionmentioning

confidence: 99%

Gene expression in term placentas is regulated more by spinal or epidural anesthesia than by late-onset preeclampsia or gestational diabetes mellitus

Lekva

Lyle

Roland

et al. 2016

Sci Rep

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Pre-eclampsia (PE) and gestational diabetes mellitus (GDM) are common complications of pregnancy, but the mechanisms underlying these disorders remain unclear. The aim was to identify the extent of altered gene expression in term placentas from pregnant women with late-onset PE and GDM compared to controls. RNAseq identified few significantly differentially regulated genes in placental biopsies between PE, GDM, or uncomplicated pregnancy (n = 10 each group). Five genes were altered in placentas from PE including 4 non-coding genes and Angiopoietin 2 (ANGPT2). No genes were significantly regulated by GDM. In contrast, many genes were significantly regulated by fetal, maternal and delivery-specific variables, particularly spinal and epidural anesthesia. We selected ANGPT2 and Chemokine (C-X-C motif) ligand 14 (CXCL14) to test with qPCR in a larger set of placentas (n = 475) and found no differences between the groups. However, regression analysis revealed a stronger association between placental ANGPT2 and CXCL14 mRNA expression and fetal, maternal and delivery-specific variables than diagnostic group. To conclude, the gene expression in term placentas are highly affected by fetal, maternal and delivery specific variables. Few regulated genes were found in late-onset PE and GDM placentas, which may suggest that these conditions could be more affected by maternal factors.The placenta is a composite structure of embryonic and maternal tissues that supplies nutrients and removes waste products from the developing embryo. It also has critical endocrine and immuno-modulatory properties. Up to 10% of pregnancies suffer from abnormal placental development and, as such, are at high risk for complications such as miscarriage, pre-eclampsia (PE), gestational diabetes mellitus (GDM), fetal growth restriction and preterm birth 1 . Pre-eclampsia is characterized by new-onset hypertension and end-organ damage after 20 weeks of gestation and affects 3-5% of all pregnancies 2 . PE has a multifactorial etiology and includes several subtypes 3 . Early-onset PE generally involves poor placental development, whereas late-onset PE is believed to arise from the interaction between a normal placenta and a damaged maternal microvasculature due, for example, to chronic hypertension or diabetes 4 . The late-onset form of PE may thus be more similar to GDM, in which pregnancy serves as a stress test in predisposed women 5,6 . GDM is defined as glucose intolerance of variable degrees first recognized during pregnancy. It may affect up to 20% of pregnant women depending on ethnicity and the diagnostic criteria used 7 .

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iTRAQ-based Proteomic Analysis Unveils ACSL4 as a Novel Potential Regulator of Human Endometrial Receptivity

Sun

et al. 2023

Endocrinology

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Competent endometrial receptivity is a prerequisite for successful embryo implantation. Identification of novel key molecules involved in endometrial receptivity is essential to better interpret human implantation and improve pregnancy rates in assisted reproduction treatment. The iTRAQ-based proteomics was performed to profile the proteomes of the prereceptive (LH + 2, n = 4) and receptive (LH + 7, n = 4) endometrial tissues. One hundred seventy-three differentially expressed proteins (DEPs) between LH + 2 and LH + 7 endometrial samples were identified. Integrated analysis of the proteomic data and published transcriptomic data was performed to identify the concordant DEPs with differential expression at both mRNA and protein levels. The protein-protein-interaction (PPI) network analysis were performed on concordant DEPs. We first identified 63 novel concordant DEPs and 5 hub proteins (ACSL4, ACSL5, COL1A1, PTGS1 and PLA2G4F) between LH + 2 and LH + 7 endometrial samples. ACSL4 was predominantly expressed in endometrial epithelial cells and its expression was significantly upregulated by progesterone in the LH + 7 endometrium and significantly downregulated in repeated implantation failure (RIF) patients. Knockdown of ACSL4 in endometrial epithelial cells induced the down-regulation of endometrial receptivity markers (HOXA10, COX2 and LIF) and the significant decrease of implantation rate during in vitro implantation analysis. This study provides the first gel-independent quantitative proteomes of the LH + 2 and LH + 7 human endometrium using iTRAQ technology. The identified concordant DEPs and hub proteins open a new avenue for future studies aimed at elucidating the underlying mechanisms governing endometrial receptivity. ACSL4 was identified as a novel regulatory molecule in the establishment of endometrial receptivity and might play important roles during implantation.

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Gene profiling of inflammatory genes in day 18 endometria from pregnant and non‐pregnant mares

Cited by 3 publications

References 35 publications

Prenatal maternal vaginal inflammation increases anxiety and alters HPA axis signalling in adult male mice

Prenatal maternal vaginal inflammation increases anxiety and alters HPA axis signalling in adult male mice

Gene expression in term placentas is regulated more by spinal or epidural anesthesia than by late-onset preeclampsia or gestational diabetes mellitus

iTRAQ-based Proteomic Analysis Unveils ACSL4 as a Novel Potential Regulator of Human Endometrial Receptivity

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