iTRAQ-based Proteomic Analysis Unveils ACSL4 as a Novel Potential Regulator of Human Endometrial Receptivity

Hu, Shuanggang; Sun, Zhe; Li, Boyu; Zhao, Hanting; Wang, Yuan; Yao, Guangxin; Li, Xinyu; Bian, Xiaolan; Li, Tin‐Chiu; Vankelecom, Hugo; Sun, Yun

doi:10.1210/endocr/bqad012

Cited by 4 publications

(2 citation statements)

References 74 publications

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“…A recent iTRAQ-based proteomics study identified PLA2G4F as a differentially expressed protein (DEPs) in mid-secretory endometrium. It is categorized as one of the top 5 hub proteins that regulate endometrial receptivity [38]. Ceruloplasmin (CP) is the sole gene with down-regulated expression in the LP phase and upregulated expression in the MS phase.…”

Section: Discussionmentioning

confidence: 99%

Endometrial Proliferative Phase-Centered View on the Transcriptome Dynamics Across the Menstrual Cycle

Apostolov,

Naydenov,

Kalinina

et al. 2024

Preprint

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The endometrium, the inner mucosal lining of the uterus, undergoes complex molecular and cellular changes across the menstrual cycle in preparation for embryo implantation. Transcrip-tome-wide analyses have mainly been utilized to study the endometrial receptivity, the prerequisite for successful implantation, with most of the studies, so far, comparing the endometrial transcriptomes between (i) secretory versus proliferative endometrium or (ii) mid-secretory versus early-secretory endometrium. In the current study, we provide the complete transcriptome description of the endometrium across the entire menstrual cycle and, for the first time, comprehensively characterize the proliferative phase of the endometrium. Our temporal transcriptome analysis includes five time points: mid-proliferative, late-proliferative (peri-ovulatory phase), early-secretory, mid-secretory, and late-secretory phases. Thus, we exhaustively unveil the transitions between the consecutive proliferative and secretory phases, highlighting their unique gene expression profiles and possible distinct biological functions. Transcriptome analysis reveals many differentially expressed genes (DEGs) across the menstrual cycle, most of which are phase-specific. As an example of the coordinated gene activity, the expression profile of his-tone-encoding genes within the HIST cluster on chromosome 6 showed an increase in cluster activity during the late-proliferative and a decline during the mid-secretory phase. Moreover, numerous DEGs are shared among all phases. In conclusion, in the current study, we delineate the endometrial proliferative phase-centered view of transcriptome dynamics across the menstrual cycle. Data analysis highlights significant transcriptomic and functional changes occurring during the late-proliferative phase – an essential transition point from the proliferative to the secretory phase. Future studies should explore how the biology of the late-proliferative phase endometrium impacts the achievement of mid-secretory endometrial receptivity or contributes to molecular aberrations leading to embryo implantation failure.

show abstract

Section: Discussionmentioning

confidence: 99%

Endometrial Proliferative Phase-Centered View on the Transcriptome Dynamics Across the Menstrual Cycle

Apostolov,

Naydenov,

Kalinina

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…A recent iTRAQ-based proteomics study identified PLA2G4F as a differentially expressed protein (DEP) in mid-secretory endometrium. It is categorized as one of the top five hub proteins that regulate endometrial receptivity [31]. Ceruloplasmin (CP) is the sole gene with downregulated expression in the LP phase and upregulated expression in the MS phase.…”

Section: Discussionmentioning

confidence: 99%

Endometrial Proliferative Phase-Centered View of Transcriptome Dynamics across the Menstrual Cycle

Apostolov,

Naydenov,

Kalinina

et al. 2024

IJMS

View full text Add to dashboard Cite

The endometrium, the inner mucosal lining of the uterus, undergoes complex molecular and cellular changes across the menstrual cycle in preparation for embryo implantation. Transcriptome-wide analyses have mainly been utilized to study endometrial receptivity, the prerequisite for successful implantation, with most studies, so far, comparing the endometrial transcriptomes between (i) secretory and proliferative endometrium or (ii) mid-secretory and early secretory endometrium. In the current study, we provide a complete transcriptome description of the endometrium across the entire menstrual cycle and, for the first time, comprehensively characterize the proliferative phase of the endometrium. Our temporal transcriptome analysis includes five time points including the mid-proliferative, late proliferative (peri-ovulatory phase), early secretory, mid-secretory, and late secretory phases. Thus, we unveil exhaustively the transitions between the consecutive proliferative and secretory phases, highlighting their unique gene expression profiles and possible distinct biological functions. The transcriptome analysis reveals many differentially expressed genes (DEGs) across the menstrual cycle, most of which are phase-specific. As an example of coordinated gene activity, the expression profile of histone-encoding genes within the HIST cluster on chromosome 6 shows an increase in cluster activity during the late proliferative and a decline during the mid-secretory phase. Moreover, numerous DEGs are shared among all phases. In conclusion, in the current study, we delineate the endometrial proliferative phase-centered view of transcriptome dynamics across the menstrual cycle. Our data analysis highlights significant transcriptomic and functional changes occurring during the late proliferative phase—an essential transition point from the proliferative phase to the secretory phase. Future studies should explore how the biology of the late proliferative phase endometrium impacts the achievement of mid-secretory endometrial receptivity or contributes to molecular aberrations leading to embryo implantation failure.

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Long-chain acyl-CoA synthetase-4 regulates endometrial decidualization through a fatty acid β-oxidation pathway rather than lipid droplet accumulation

Zhang,

Sun,

Dong

et al. 2024

Molecular Metabolism

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iTRAQ-based Proteomic Analysis Unveils ACSL4 as a Novel Potential Regulator of Human Endometrial Receptivity

Cited by 4 publications

References 74 publications

Endometrial Proliferative Phase-Centered View on the Transcriptome Dynamics Across the Menstrual Cycle

Endometrial Proliferative Phase-Centered View on the Transcriptome Dynamics Across the Menstrual Cycle

Endometrial Proliferative Phase-Centered View of Transcriptome Dynamics across the Menstrual Cycle

Long-chain acyl-CoA synthetase-4 regulates endometrial decidualization through a fatty acid β-oxidation pathway rather than lipid droplet accumulation

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