2018
DOI: 10.1073/pnas.1722588115
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Phospholipase A activity of adenylate cyclase toxin?

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Cited by 5 publications
(10 citation statements)
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References 9 publications
(4 reference statements)
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“…A putative contamination of our toxin by OMPLA seems, therefore, unconvincing, and even if it happened it would be an "inactive contaminant." Regarding the supposition of loss of RTX domain function of ACT-D1079A by protein degradation, mentioned in the Masin et al (1) letter, our data (CD, binding to liposomes and to macrophages, and calcium response) do not support it. In relation to the methyl arachidonyl fluorophosphonate (MAFP)-treated ACT data in cells, we show that control ACT (no MAFP) treated identically (dialysis) retains its PLA activity in cells, and the inhibitory effect of MAFP on the ACT-PLA activity was also proved in the PLA assays in vitro, in which treatment of the samples did not include the dialysis step.…”
contrasting
confidence: 92%
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“…A putative contamination of our toxin by OMPLA seems, therefore, unconvincing, and even if it happened it would be an "inactive contaminant." Regarding the supposition of loss of RTX domain function of ACT-D1079A by protein degradation, mentioned in the Masin et al (1) letter, our data (CD, binding to liposomes and to macrophages, and calcium response) do not support it. In relation to the methyl arachidonyl fluorophosphonate (MAFP)-treated ACT data in cells, we show that control ACT (no MAFP) treated identically (dialysis) retains its PLA activity in cells, and the inhibitory effect of MAFP on the ACT-PLA activity was also proved in the PLA assays in vitro, in which treatment of the samples did not include the dialysis step.…”
contrasting
confidence: 92%
“…OMPLA has lysophospholipase activity (3-6), and has two calcium binding sites with micromolar affinity (7). What Masin et al (1) argue involves that the "putative contaminant" is not randomly present in our preparations, but rather that it would have to be, precisely, "more" present in ACT, and "less" present in S606A, and absent (?) in D1079A preparations, to fit our data.…”
mentioning
confidence: 92%
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“…USA . [ 37 ]). Although Gonzáles-Bullón et al claimed to have discovered a new enzymatic activity of CyaA, the enzyme kinetics of the putative PLA activity of CyaA were not shown.…”
Section: Introductionmentioning
confidence: 99%