Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Mitra, Jyotirmoy; Bhattacharyya, Debasish

doi:10.1016/j.toxicon.2014.06.004

Cited by 30 publications

(23 citation statements)

References 54 publications

(59 reference statements)

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“…ATPase, ADPase, AMPase, and PDE are poorly characterized, high molecular mass (>50 kDa) proteins of snake venom 20 , 35 . Our biochemical assays showed that ECV contains ATPase (6.3 ± 0.006 × 10 4 U/mg), ADPase (3.6 ± 0.300 × 10 4 U/mg), AMPase/nucleotidase (8.2 ± 0.4 × 10 3 U/mg), and PDE (18.6 ± 0.08 U/mg) enzyme activities.…”

Section: Resultsmentioning

confidence: 99%

Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation

Patra

Kalita

Chanda

et al. 2017

Sci Rep

140

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The proteome composition of Echis carinatus carinatus venom (ECV) from India was studied for the first time by tandem mass spectrometry analysis. A total of 90, 47, and 22 distinct enzymatic and non-enzymatic proteins belonging to 15, 10, and 6 snake venom protein families were identified in ECV by searching the ESI-LC-MS/MS data against non-redundant protein databases of Viperidae (taxid 8689), Echis (taxid 8699) and Echis carinatus (taxid 40353), respectively. However, analysis of MS/MS data against the Transcriptome Shotgun Assembly sequences (87 entries) of conger E. coloratus identified only 14 proteins in ECV. Snake venom metalloproteases and snaclecs, the most abundant enzymatic and non-enzymatic proteins, respectively in ECV account for defibrinogenation and the strong in vitro pro-coagulant activity. Further, glutaminyl cyclase, aspartic protease, aminopeptidase, phospholipase B, vascular endothelial growth factor, and nerve growth factor were reported for the first time in ECV. The proteome composition of ECV was well correlated with its biochemical and pharmacological properties and clinical manifestations observed in Echis envenomed patients. Neutralization of enzymes and pharmacological properties of ECV, and immuno-cross-reactivity studies unequivocally point to the poor recognition of <20 kDa ECV proteins, such as PLA2, subunits of snaclec, and disintegrin by commercial polyvalent antivenom.

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Section: Resultsmentioning

confidence: 99%

Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation

Patra

Kalita

Chanda

et al. 2017

Sci Rep

140

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“…Venom phosphodiesterase is also likely to contribute to the haemostatic impairment in victims of D. siamensis envenoming. This enzyme hydrolyses ADP, strongly inhibiting ADP-induced platelet aggregation in human platelet-rich plasma [ 73 ]. In addition, it has been suggested that the disturbance in extracellular nucleotides and purines levels generated from endogenous precursors may potentiate venom-induced hypotension and paralysis via purine receptors [ 74 ].…”

Section: Resultsmentioning

confidence: 99%

Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center

et al. 2018

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The venom proteome of Siamese Russell’s viper from Taiwan, alongside complementary in vivo lethality neutralization assay and in vitro third-generation antivenomics assessment of the preclinical efficacy of the homologous antivenom manufactured in Taiwan CDC’s Vaccine Center, are here reported. Taiwanese Russell’s viper venom proteome comprised 25 distinct gene products, with the heterodimeric PLA2 viperotoxin-F representing the most abundant toxin (47.5% of total venom proteome). Coagulation FV-activating serine proteinase (RVV-V, 14%), the PIV-SVMP activator of FX (RVV-FX, 8.5%), and less abundant toxins from nine protein families, make up its venom proteome. Venom composition-pathology correlations of D. siamensis envenomings in Taiwan are discussed. The lethal effect of Taiwanese D. siamensis venom was 0.47 mg/g mouse. Antivenomics-guided assessment of the toxin recognition landscape of the Taiwanese Russell’s viper antivenom, in conjunction with complementary in vivo neutralization analysis, informed the antivenom’s maximal toxin immunorecognition ability (14 mg total venom proteins/vial), neutralization capacity (6.5 mg venom/vial), and relative content of lethality neutralizing antibodies (46.5% of the toxin-binding F(ab’)2 antibodies). The antivenomics analysis also revealed suboptimal aspects of the CDC-Taiwan antivenom. Strategies to improve them are suggested.

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“…Furthermore, the release of adenosine may cause platelet aggregation inhibition, thereby worsening the hemostatic derangement in Russell’s viper envenomation 85 . Also, it has been shown that PDE could strongly inhibit ADP-induced platelet aggregation in human plasma, hence potentiating the hemotoxic effect of the venom 86 . On the other hand, CRiSP (detected in Ds-Guangxi venom) was similar to ablomin of Gloydius blomhoffii , a minor venom protein that targets L-type voltage-gated calcium channels (Ca v ) and blocks smooth muscle contraction 87 .…”

Section: Discussionmentioning

confidence: 99%

Venom proteomics and antivenom neutralization for the Chinese eastern Russell’s viper, Daboia siamensis from Guangxi and Taiwan

Tan

2018

Sci Rep

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The eastern Russell’s viper (Daboia siamensis) causes primarily hemotoxic envenomation. Applying shotgun proteomic approach, the present study unveiled the protein complexity and geographical variation of eastern D. siamensis venoms originated from Guangxi and Taiwan. The snake venoms from the two geographical locales shared comparable expression of major proteins notwithstanding variability in their toxin proteoforms. More than 90% of total venom proteins belong to the toxin families of Kunitz-type serine protease inhibitor, phospholipase A2, C-type lectin/lectin-like protein, serine protease and metalloproteinase. Daboia siamensis Monovalent Antivenom produced in Taiwan (DsMAV-Taiwan) was immunoreactive toward the Guangxi D. siamensis venom, and effectively neutralized the venom lethality at a potency of 1.41 mg venom per ml antivenom. This was corroborated by the antivenom effective neutralization against the venom procoagulant (ED = 0.044 ± 0.002 µl, 2.03 ± 0.12 mg/ml) and hemorrhagic (ED50 = 0.871 ± 0.159 µl, 7.85 ± 3.70 mg/ml) effects. The hetero-specific Chinese pit viper antivenoms i.e. Deinagkistrodon acutus Monovalent Antivenom and Gloydius brevicaudus Monovalent Antivenom showed negligible immunoreactivity and poor neutralization against the Guangxi D. siamensis venom. The findings suggest the need for improving treatment of D. siamensis envenomation in the region through the production and the use of appropriate antivenom.

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Phosphodiesterase from Daboia russelli russelli venom: Purification, partial characterization and inhibition of platelet aggregation

Cited by 30 publications

References 54 publications

Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation

Proteomics and antivenomics of Echis carinatus carinatus venom: Correlation with pharmacological properties and pathophysiology of envenomation

Translational Venomics: Third-Generation Antivenomics of Anti-Siamese Russell’s Viper, Daboia siamensis, Antivenom Manufactured in Taiwan CDC’s Vaccine Center

Venom proteomics and antivenom neutralization for the Chinese eastern Russell’s viper, Daboia siamensis from Guangxi and Taiwan

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