Phosphatodylethanolamine liposomes: drug delivery, gene transfer and immunodiagnostic applications

Litzinger, David C.; Huang, Leaf

doi:10.1016/0304-4157(92)90039-d

Cited by 347 publications

(167 citation statements)

References 176 publications

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“…Our results indicate that the cholesterol requirement is circumvented by the presence of PEG-phospholipid. The high stability of DOPE liposomes with PEG-phospholipids is also in contrast to DOPE liposomes stabilized with lowmolecular weight amphiphiles, which leak in the presence of plasma or serum [23].…”

Section: Resultsmentioning

confidence: 99%

“…The structure of the purified At physiological pH and ionic strength DOPE does not form bilayers, but rather exists in an inverted hexagonal (HII) phase. To produce liposomes from a DOPE-rich lipid, it must be stabilized in a bilayer phase by an amphiphile with bulky and/or repulsing hydrophilic moieties [23], the requirement perfectly satisfied by PEG-phospholipid conjugates. DOPE readily formed liposomes with either mPEG-DSPE or mPEG-DTP-DSPE (2.9 or 5.7 mol% of PL).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Liposomes with detachable polymer coating: destabilization and fusion of dioleoylphosphatidylethanolamine vesicles triggered by cleavage of surface‐grafted poly(ethylene glycol)

Kirpotin

Hong

Mullah³

et al. 1996

FEBS Letters

194

112

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Plasma-stable liposomes (100 nm) were prepared from dioleoylphosphatidylethanolamine (DOPE) and 3--6 tool% of a new disulfide-linked poly(ethylene glycol)-phospbolipid conjugate (mPEG-DTP-DSPE). In contrast to similar preparations containing non-cleavable PEG-phospholipid conjugate, thiolytic cleavage of the grafted polymer chains facilitated rapid and complete release of the liposome contents. Furthermore, the detachment of PEG from DOPE liposomes resulted in liposomal fusion. Finally, while formulation of pH-sensitive DOPE/ cholesterol hemisuccinate liposomes with mPEG-DTP-DSPE abolished the pH sensitivity, cleavage of the PEG chains completely restored this property. These are the first examples of new useful properties of liposomes grafted with cleavable polymer.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Liposomes with detachable polymer coating: destabilization and fusion of dioleoylphosphatidylethanolamine vesicles triggered by cleavage of surface‐grafted poly(ethylene glycol)

Kirpotin

Hong

Mullah³

et al. 1996

FEBS Letters

194

112

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“…It is not favorable for the formation of a bilayer structure by itself under physiological conditions. 25 DOPE can form a bilayer with other lipids such as DOTAP, however, the resulting liposomes are relatively unstable and could undergo destabilization upon changes in pH, ion strength, temperature, etc. 25 Likewise, it is speculated that such unstable liposomes tend to form loose complexes with DNA in which DNA is poorly protected by cationic lipids.…”

Section: Discussionmentioning

confidence: 99%

“…25 DOPE can form a bilayer with other lipids such as DOTAP, however, the resulting liposomes are relatively unstable and could undergo destabilization upon changes in pH, ion strength, temperature, etc. 25 Likewise, it is speculated that such unstable liposomes tend to form loose complexes with DNA in which DNA is poorly protected by cationic lipids. This was confirmed in EtBr binding assay; more DNA in the DOPE-containing lipoplexes was accessible for interaction with EtBr as compared with the other two formulations (Figure 4a).…”

Section: Discussionmentioning

confidence: 99%

Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: implications for intravenous lipofection

et al. 1999

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Intravenous gene delivery via cationic lipidic vectors gives in this study. Yet, vectors of different lipid compositions systemic gene expression particularly in the lung. In order vary greatly in the rate of disintegration. There is an inverse to understand the mechanism of intravenous lipofection, a correlation between the disintegration rate of lipidic vectors systematic study was performed to investigate the interacand their in vivo transfection efficiency. Vectors with a rapid tions of lipidic vectors with mouse serum emphasizing how rate of disintegration such as those containing dioleoylserum affects the biophysical and biological properties of phosphatidylethanolamine (DOPE) poorly stayed in the vectors of different lipid compositions. Results from this lung and were barely active in transfecting cells. In constudy showed that lipidic vectors underwent dynamic trast, cholesterol-containing vectors that had a rapid aggrechanges in their characteristics after exposure to serum.gation and a slow disintegration were highly efficient in Addition of lipidic vectors into serum resulted in an immeditransfecting cells in vivo. The results of this study explain ate aggregation of vectors. Prolonged incubation of lipidic why cationic lipidic vectors of different lipid compositions vectors with serum led to vector disintegration as shown in have a dramatic difference in their in vivo transfection turbidity study, sucrose-gradient centrifugation analysis efficiency. These results also suggest that the study of the and fluorescence resonance energy transfer (FRET) study.interactions of lipidic vectors with serum may serve as a Vector disintegration was associated with DNA release and predictive model for the in vivo efficiency of a lipidic vector. degradation as shown in EtBr intercalation assay and DNA Further study of the numerous interactions of lipidic vectors digestion study. Serum-induced disintegration of vectors is with serum might lead to the development of a vector which a general phenomenon for all cationic lipidic vectors tested can deliver a gene to target cells in a tissue-specific manner.

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“…Although the uptake of cationic liposomes has been suggested to be via endocytosis and the endosomes, 30,31 the exact mechanism of cationic lipid-mediated gene transfer has not been elucidated. Much of the recent development on new cationic lipid formulations has been empirical and requires the systematic screening for optimal activity in the respective target cells/tissues.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a novel series of cationic lipids that can act as efficient gene delivery vehicles through systematic heterocyclic substitution of cholesterol derivatives

Gao

Hui

2001

Gene Ther

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The synthesis of a series of novel cationic lipids through the systematic substitution of cholesterol derivatives that could greatly enhance the delivery and expression of plasmid DNA in vitro and in vivo is described. Two of the newly synthesized lipids, designated as NCC4 and NCC10, were chosen to be studied in detail and gave much higher levels of gene expression than that which could be obtained with some of the conventional cationic polymers and cationic liposomes.

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Phosphatodylethanolamine liposomes: drug delivery, gene transfer and immunodiagnostic applications

Cited by 347 publications

References 176 publications

Liposomes with detachable polymer coating: destabilization and fusion of dioleoylphosphatidylethanolamine vesicles triggered by cleavage of surface‐grafted poly(ethylene glycol)

Liposomes with detachable polymer coating: destabilization and fusion of dioleoylphosphatidylethanolamine vesicles triggered by cleavage of surface‐grafted poly(ethylene glycol)

Dynamic changes in the characteristics of cationic lipidic vectors after exposure to mouse serum: implications for intravenous lipofection

Synthesis of a novel series of cationic lipids that can act as efficient gene delivery vehicles through systematic heterocyclic substitution of cholesterol derivatives

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