Phosphatidic acid plays key roles regulating plant development and stress responses

Yao, Hongyan; Hou, Xue

doi:10.1111/jipb.12655

Cited by 111 publications

(81 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PLDf2 produces PA, a key signaling molecule involved in various physiological processes, by directly binding to various target proteins (Yao & Xue, 2018). PLDf2 and PA mediate auxin transport and distribution through regulating the cycling of PIN2 (Li & Xue, 2007).…”

Section: New Phytologistmentioning

confidence: 99%

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Lin

Yao

et al. 2019

New Phytologist

View full text Add to dashboard Cite

Summary Root hair development is crucial for phosphate absorption, but how phosphorus deficiency affects root hair initiation and elongation remains unclear. We demonstrated the roles of auxin efflux carrier PIN‐FORMED2 (PIN2) and phospholipase D (PLD)‐derived phosphatidic acid (PA), a key signaling molecule, in promoting root hair development in Arabidopsis thaliana under a low phosphate (LP) condition. Root hair elongation under LP conditions was greatly suppressed in pin2 mutant or under treatment with a PLDζ2‐specific inhibitor, revealing that PIN2 and polar auxin transport and PLDζ2‐PA are crucial in LP responses. PIN2 was accumulated and degraded in the vacuole under a normal phosphate (NP) condition, whereas its vacuolar accumulation was suppressed under the LP or NP plus PA conditions. Vacuolar accumulation of PIN2 was increased in pldζ2 mutants under LP conditions. Increased or decreased PIN2 vacuolar accumulation is not observed in sorting nexin1 (snx1) mutant, indicating that vacuolar accumulation of PIN2 is mediated by SNX1 and the relevant trafficking process. PA binds to SNX1 and promotes its accumulation at the plasma membrane, especially under LP conditions, and hence promotes root hair development by suppressing the vacuolar degradation of PIN2. We uncovered a link between PLD‐derived PA and SNX1‐dependent vacuolar degradation of PIN2 in regulating root hair development under phosphorus deficiency.

show abstract

Section: New Phytologistmentioning

confidence: 99%

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Lin

Yao

et al. 2019

New Phytologist

View full text Add to dashboard Cite

show abstract

“…The regulation of CME by PA is putatively achieved through different modes of action. On the one hand, PA acts as a signal for cytosolic proteins to be recruited to the plasma membrane (PM; Testerink & Munnik, 2011); on the other hand, PA increases the negative curvature at the cytoplasmic leaflet of membranes thereby facilitating membrane fusion and fission (Kooijman, Chupin, & Burger, 2003;Yao & Xue, 2018).…”

mentioning

confidence: 99%

Halotropism requires phospholipase Dζ1‐mediated modulation of cellular polarity of auxin transport carriers

Korver

Berg

Meyer

et al. 2019

Plant Cell & Environment

View full text Add to dashboard Cite

Endocytosis and relocalization of auxin carriers represent important mechanisms for adaptive plant growth and developmental responses. Both root gravitropism and halotropism have been shown to be dependent on relocalization of auxin transporters. Following their homology to mammalian phospholipase Ds (PLDs), plant PLDζ‐type enzymes are likely candidates to regulate auxin carrier endocytosis. We investigated root tropic responses for an Arabidopsis pldζ1‐KO mutant and its effect on the dynamics of two auxin transporters during salt stress, that is, PIN2 and AUX1. We found altered root growth and halotropic and gravitropic responses in the absence of PLDζ1 and report a role for PLDζ1 in the polar localization of PIN2. Additionally, irrespective of the genetic background, salt stress induced changes in AUX1 polarity. Utilizing our previous computational model, we found that these novel salt‐induced AUX1 changes contribute to halotropic auxin asymmetry. We also report the formation of “osmotic stress‐induced membrane structures.” These large membrane structures are formed at the plasma membrane shortly after NaCl or sorbitol treatment and have a prolonged presence in a pldζ1 mutant. Taken together, these results show a crucial role for PLDζ1 in both ionic and osmotic stress‐induced auxin carrier dynamics during salt stress.

show abstract

“…Recent studies indicate that GAPC could perform other functions in plants, which are not related to its catalytic activity. In addition, it has a central role in the abiotic stress response to cold [13], cadmium [17] and salt [14,18], and GAPC was suggested as a moonlighting (multifunctional) protein in plants [15].…”

Section: Introductionmentioning

confidence: 99%

Characterization of LcGAPC and its transcriptional response to salt and alkali stress in two ecotypes of Leymus chinensis (Trin.) Tzvelev

Meng

Yang

et al. 2020

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a housekeeping protein which plays various roles in non-metabolic processes in addition to its role in glucose catabolism. There are several GAPDH isoforms in plant cells. In this study, we cloned the full-length cDNA encoding the isoform GAPC in two ecotypes of Leymus chinensis (Trin.) Tzvelev, gray-green type and yellow-green type. The LcGAPC sequence includes an open reading frame (ORF) of 1014 bp encoding 337 amino acids. The predicted molecular weight is 36.5 kDa, and the pI is 6.19. The mRNA expression of LcGAPC decreased at 24 h and then increased significantly after 7 d after 400 mmol/L NaCl stress. At 200 mmol/L mixed alkali stress (NaHCO 3 :Na 2 CO 3 ¼9:1), the LcGAPC expression level gradually increased as time increased in the two ecotypes of L. chinensis. The results suggested that LcGAPC is a stress-inducible gene that might play a role in the salt and alkali stress response. This study provided a basis to further study the mechanism of expression characteristics under salt and alkali stress conditions of L. chinensis. ARTICLE HISTORY

show abstract

Phosphatidic acid plays key roles regulating plant development and stress responses

Cited by 111 publications

References 101 publications

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Phospholipase D‐derived phosphatidic acid promotes root hair development under phosphorus deficiency by suppressing vacuolar degradation of PIN‐FORMED2

Halotropism requires phospholipase Dζ1‐mediated modulation of cellular polarity of auxin transport carriers

Characterization of LcGAPC and its transcriptional response to salt and alkali stress in two ecotypes of Leymus chinensis (Trin.) Tzvelev

Contact Info

Product

Resources

About