Phosphate Solubilizing Yeast Isolated and Characterized from Teff Rhizosphere Soil Collected from Gojam; Ethiopia

Abstract

Phosphorous is an essential macronutrient for plant growth and development. About 95-99% present in soil insoluble form. Phosphate solubilizing microorganisms can increase soil phosphate solubility and availability. This study was aimed to identify and evaluate phosphate solubilizing yeast from Teff rhizosphere soil. Yeasts were identified using Biolog Micro station identification system. Yeast isolates were screened and transferred to biolog universal yeast agar media. Pure yeast cells were suspended in sterile water at 49+2 turbidity measured by biolog turbidimeter. 100μ-L transferred from each suspension into 96 wells of the biology yeast micro Plate tagged with different carbon source and incubated at 26˚C for 24 to 72 h and read by micro station at a single wavelength of 590 nm, results were recorded and processed for identification by micro log3 software ver. 4.20.05. Above 0.5 similarities index value is acceptable species identification. Therefore biolog microstations identify nine yeast species with full species identity. The identified yeasts were tested for phosphate solubilization by the Pikovskaya's agar (PVK) selective media. Nine yeast species were positive in phosphate solubilizing ability. Phichia norvegensis, Cryptococcus albidus var aerius, Candida etchellsii, Cryptococcus albidus var albidus, Rhodotrula aurantiaca A, Rhodotorula aurantiaca B, Cryptococcus luteolus, Cryptococcus albidus var diffluens,Cryptococcus terreus A. At 15 days incubation their phosphate solubilizing index (PSI) ranges 1.72-3.35. Phichia norvegensis and Cryptococcus albidus var aerius were superior in phosphate solubilization 3.35 and 3.2 SPI respectively. Therefore these species can be candidated and exploited after further evaluation as bio fertilizers for teff productivity.

Evaluation of Multifarious Plant Growth Promoting Trials of Yeast Isolated from the Soil of Assam Tea (Camellia sinensis var. assamica) Plantations in Northern Thailand

“…The results indicated that only two yeast species, P. laurentii and W. anomalus , were able to solubilize the insoluble form of phosphate (Ca 3 (PO 4 ) 2 ), and zinc (ZnO and ZnCO 3 ). These results were similar to those of other previous studies which reported on the phosphate and zinc-solubilizing abilities of both yeast species [ 26 , 65 , 80 , 81 , 82 ]. Moreover, some species of yeast in the genera Aureobasidium, Barnettozyma, Candida, Holtermanniella, Lachancea, Pichia, Rhodotrula, Saccharomyces, Torulaspora have been recognized for their phosphate and zinc-solubilizing abilities [ 26 , 29 , 80 , 81 , 82 , 83 , 84 ].…”

“…These results were similar to those of other previous studies which reported on the phosphate and zinc-solubilizing abilities of both yeast species [ 26 , 65 , 80 , 81 , 82 ]. Moreover, some species of yeast in the genera Aureobasidium, Barnettozyma, Candida, Holtermanniella, Lachancea, Pichia, Rhodotrula, Saccharomyces, Torulaspora have been recognized for their phosphate and zinc-solubilizing abilities [ 26 , 29 , 80 , 81 , 82 , 83 , 84 ]. Additionally, a number of previous studies have reported that the insoluble mineral solubilization ability of yeast cultures can vary depending on the type of mineral and yeast species as well as the particular yeast strain [ 26 , 29 , 83 , 84 ].…”

Tobacco Plant Growth-Promoting and Antifungal Activities of Three Endophytic Yeast Strains

“…Several yeasts are known to produce mycocins (extracellular proteins) involved in inhibition of β -glucan synthesis in the cell wall of sensitive strains, which cause the death of the other species of yeast, moulds, and bacteria [ 16 ]. Yeast is involved in the process of dissolving soil-insoluble phosphates, transforming complex organic biomass into mineral compounds used by plants, and the synthesis of growth factors such as amino acids, vitamins, and enzymes [ 17 ]. There have been an increased number of studies concerning the isolation of endophytic yeasts that stimulate plant growth [ 18 ].…”

Phosphate Solubilizing Bacteria in Optimizing Phosphorous Acquisition and Crop Production

“…As an indicator, bacteria strain as inoculants for different crop production is an appreciating starting research done by Ethiopian researcher. Hence, Gizaw B, et al [94] reported that Phichia norvegensis and Cryptococcus albidus var aerius were superior in phosphate solubilization and recommended as bio fertilizers for teff productivity. Additionally, Muleta D et al [95] also suggested that Pseudomonas spp.…”