Phosphate solubilization by Trichoderma koningiopsis (NBRI-PR5) under abiotic stress conditions

Tandon, Ashmita; Fatima, Touseef; Anshu, .; Shukla, Deepali; Tripathi, Pratibha; Srivastava, Suchi; Singh, Poonam

doi:10.1016/j.jksus.2019.02.001

Cited by 53 publications

(31 citation statements)

References 36 publications

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“…Thus, a correlation between the decrease in pH and the increase in P solubilization influences the biomass increase of the lateral roots 12 and consequently increases the surface of P absorption by plants. Tandon (2019) demonstrated that by alkalinizing the medium in the phosphorus solubilization process, mycelial production and phosphatase activity by Trichoderma decreased significantly, which contributes to the importance of pH in the phosphorus solubilization process 53 . Combined with another mechanism that can be important in the formation of the root system is the production of metabolites, such as auxins and ethylene, produced by a range of Trichoderma species 49,54 .…”

Section: Discussionmentioning

confidence: 99%

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Bononi

Chiaramonte

Pansa

et al. 2020

Sci Rep

154

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Acidic soils rapidly retain applied phosphorus fertilizers and consequently present low availability of this nutrient to plants. the use of phosphate-solubilizing microorganisms to help plant phosphorus (P) absorption is a promising sustainable strategy for managing P deficiencies in agricultural soils. Trichoderma strains have been one of the most studied filamentous fungi for improving the production and development of several crop species mainly due to their capability for symbiotic associations and their ability to control soil-borne plant diseases. Thus, this work sought to bioprospect Trichoderma strains from the Amazon rainforest capable of solubilizing/mineralizing soil phosphate and promoting soybean growth. Soybean plants inoculated with selected Trichoderma strains were cultivated in soil under greenhouse conditions and under a gradient of rock phosphate and triple superphosphate. As a result, 19.5% of the isolated Trichoderma strains were able to solubilize phosphate. In addition, those strains produced different organic acids during the solubilization process. Trichoderma spp. strains showed positive responses in the promotion of soybean growth-from 2.1% to 41.1%-as well as in the efficiency of P uptake-up to 141%. These results reveal the potential of Trichoderma spp. from the Amazon biome as promising biofertilizer agents.The high demand for fertilizers used in Brazilian agriculture is a result of the growing population, which necessitates an increase in food production 1 . Brazil is the second-largest supplier of food and agricultural products and expected to be the leading producer of food to meet global demand in the near future 2 . Thus, applications of fertilizer are a routine activity in agricultural production as an attempt to promote crop growth to increase productivity. The requirement of fertilizers in the field results in the accumulation of those inputs in soils and water and, therefore, environmental pollution, causing problems to human and animal health 1,3 . In the future, Brazilian agriculture has to identify alternatives to reduce its dependence on chemical fertilizers while at the same time functioning in a lucrative and more sustainable way 4 .A range of nutrients is important for plant growth 5 , but the ones that limit agricultural production the most are nitrogen and phosphorus, which are important in the initial development of the plant 6,7 . Nitrogen fertilization in Brazil has decreased significantly with use of symbiotic associations with nitrogen-fixing bacteria 8,9 . However, Brazilian agriculture continues to depend on chemical phosphate fertilization 4 . The role of P in the plant is associated with three essential biochemical processes: energy production, respiration, and photosynthesis. P is also involved in enzymatic processes and is a component of nucleic acids and cell membranes [10][11][12][13] . Phosphorus is generally found in the lowest concentration in the soil, 0.01%, compared to 0.14% of nitrogen, mainly in tropical and subtropical regions 14,15 . Although ...

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Section: Discussionmentioning

confidence: 99%

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Bononi

Chiaramonte

Pansa

et al. 2020

Sci Rep

154

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“…Data were statistically analyzed using analysis of variance and comparisons of means at a 5% level of significance with a Duncan's multiple range test analysis performed with one-way analysis of variance using IBM SPSS Statistics v25.0 software. Amplicons were scored for their presence (1) or absence (0) in each strain. Cluster analysis of the binary data was performed using the NTSYS-pc v.2.1 program [62].…”

Section: Discussionmentioning

confidence: 99%

“…The variation of clear zones around the fungal growth shown in Figure 4 might be due to their ability to produce organic acids that can reduce pH and enhance the phosphates solubilisation as well as some of micro-and macro-nutrients [12,80,81]. Moreover, one study [1] reported that T. koningiopsis could solubilize phosphate under stress conditions such as alkalinity and drought by producing organic acids, accumulated polyphosphate in its mycelia, and produced alkaline phosphatase enzyme. One paper [82] suggested three possible mechanisms for phosphate solubilization by Trichoderma species-acidification of the microenvironment, production of chelating compounds, and redox activity-but, this paper denied the secretion of organic acids by Trichoderma strains, namely oxalic, citric, DL-malic, succinic, DL-lactic, and fumaric acids.…”

Section: Ability To Solubilize Insoluble Phosphatementioning

confidence: 99%

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Hewedy

Lateif

Seleiman

et al. 2020

Biology

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Trichoderma species are known as excellent biocontrol agents against soil-borne pathogens that cause considerable crop losses. Eight strains of Trichoderma were isolated from five Egyptian regions. They identified based on translation elongation factor-1α (TEF1) sequencing as four different Trichoderma species: Trichoderma asperellum, Trichoderma harzianum, Trichoderma viride, and Trichoderma longibrachiatum. Optimal growth conditions (temperature and media), and the phosphate solubilization capability of Trichoderma strains were evaluated in vitro. Further, the ability of these strains to antagonize Fusarium solani, Macrophomina phaseolina, and Fusarium graminearum was also evaluated. The results revealed that Trichoderma harzianum (Th6) exhibited the highest antagonistic ability against F. solani, M. phaseolina and F. graminearum with inhibition rates of 71.42%, 72.97%, and 84.61%, respectively. Trichoderma viride (Tv8) exhibited the lowest antagonism against the same pathogens with inhibition rates of 50%, 64% and 69.23%, respectively. Simple-sequence repeats (SSRs) and random amplified polymorphic DNA (RAPD) markers were used to evaluate the genetic variability of the Trichoderma strains. The results revealed that of 45 RAPD amplified bands, 36 bands (80%) were polymorphic and of SSRs amplified 36 bands, 31 bands (86.11%) were polymorphic. The amplification of calmodulin and β-1,3-endoglucanase was noted at 500 bp and 230 bp, respectively. Data indicated that T. viride (Tv8) had the highest phosphate solubilization index (10.0 mm), while T. harzianum (Th6) had the lowest phosphate solubilization index (4.0 mm). In conclusion, T. harzianum (Th6) had the highest antagonistic activity in dual culture assay along with the growth rate; while T. viride (Tv8) had the highest phosphate solubilization activity. There are still gaps in obtaining new formulations, selecting potent Trichoderma strains to confirm disease control in planta. For improving Trichoderma recommendation in the organic agricultural system and sustaining the fertility of the soil, the field application of highly antagonistic biocontrol agents in different types of soil and plant species will be the first approach toward bio-pesticide treatments along with bio-fertilizer inoculation. Furthermore, secondary metabolites will be investigated for the most promising strains with the combination of different pathogens and application timing.

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“…Similarly, phosphate solubilisation by Trichoderma species has been described both in vitro and in vivo [52][53][54]. Recently, Tandon et al [55] evaluated P solubilisation of different Trichoderma koningiopsis isolates under abiotic stress conditions and determined a range from 1.6 to 71 µg mL −1 . Bononi et al [12] found that Trichoderma isolated from soils of the Amazon rainforest demonstrated a high potential for phosphate solubilisation, which ranged from 51.7 to 90.3% 10 days after inoculation.…”

Section: Discussionmentioning

confidence: 99%

Role of Trichoderma aggressivum f. europaeum as Plant-Growth Promoter in Horticulture

et al. 2020

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The main objective of this study was to determine the capacity of Trichoderma aggressivum f. europaeum to promote pepper and tomato seedling growth compared to that of T. saturnisporum, a species recently characterised as a biostimulant. Consequently, in vitro seed germination and seedling growth tests were performed under commercial plant nursery conditions. Additionally, the effects of different doses and a mixture of both species on seedling growth under plant nursery and subsequently under greenhouse conditions were determined. Furthermore, mass production of spores was determined in different substrates, and their siderophore and indole acetic acid production and phosphate (P) solubilisation capacity were also determined. Direct application of Trichoderma aggressivum f. europaeum to seeds in vitro neither increases the percentage of pepper and tomato seed germination nor improves their vigour index. However, substrate irrigation using different doses under commercial plant nursery conditions increases the quality of tomato and pepper seedlings. Tomato roots increased by 66.66% at doses of 106 spores per plant. Applying T. aggressivum f. europaeum or T. saturnisporum under plant nursery conditions added value to seedlings because their growth-promoting effect is maintained under greenhouse conditions up to three months after transplantation. The combined application of the two species had no beneficial effect in relation to that of the control. The present study demonstrates the biostimulant capacity of T. aggressivum f. europaeum in pepper and tomato plants under commercial plant nursery and greenhouse conditions.

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Phosphate solubilization by Trichoderma koningiopsis (NBRI-PR5) under abiotic stress conditions

Cited by 53 publications

References 36 publications

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Phosphorus-solubilizing Trichoderma spp. from Amazon soils improve soybean plant growth

Phylogenetic Diversity of Trichoderma Strains and Their Antagonistic Potential against Soil-Borne Pathogens under Stress Conditions

Role of Trichoderma aggressivum f. europaeum as Plant-Growth Promoter in Horticulture

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